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The map label for this gene is cspA [H]

Identifier: 195977285

GI number: 195977285

Start: 150535

End: 150738

Strand: Direct

Name: cspA [H]

Synonym: Sez_0138

Alternate gene names: 195977285

Gene position: 150535-150738 (Clockwise)

Preceding gene: 195977281

Following gene: 195977286

Centisome position: 7.44

GC content: 42.16

Gene sequence:

>204_bases
ATGGCACAAGGTACAGTCAAGTGGTTTAATGCTGAAAAGGGCTTTGGCTTTATTTCAACTGAAAATGGTCAAGATGTTTT
TGCACATTTTTCAGCAATCCAGTCTGACGGCTTTAGATCGTTAGACGAGGGACAAAGGGTGACGTTTGATGTTGAAGACG
GACAGCGCGGTCCTCAGGCAGTAAATATCACTAAATTGTCTTAA

Upstream 100 bases:

>100_bases
CACTGGGTGAGTAAATTAGTTTTTGAATTAGTAGTTGTGTTTTGGCATCTTTATTTCTCTCTATGTTTACAAATGCGTTA
TAGAAAGGAAGTGGATGACT

Downstream 100 bases:

>100_bases
TCTAGTGAGCAGATAATAGGGGATGGGTGTTGTCAATGGCGCCTGTCTTTTTACATTCTAGTGATTTCCATTTGCTTTTA
GTGACTGCTATTTGATATAA

Product: major cold-shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQSDGFRSLDEGQRVTFDVEDGQRGPQAVNITKLS

Sequences:

>Translated_67_residues
MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQSDGFRSLDEGQRVTFDVEDGQRGPQAVNITKLS
>Mature_66_residues
AQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQSDGFRSLDEGQRVTFDVEDGQRGPQAVNITKLS

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=64.0625, Blast_Score=92, Evalue=9e-21,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=60, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=60.9375, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=58.4615384615385, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=63.9344262295082, Blast_Score=85, Evalue=9e-19,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=57.8125, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=55.2238805970149, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7369; Mature: 7238

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQSDGFRSLDEGQRVTFDVEDGQRGPQA
CCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCE
VNITKLS
EEEEECC
>Mature Secondary Structure 
AQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQSDGFRSLDEGQRVTFDVEDGQRGPQA
CCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCE
VNITKLS
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]