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The map label for this gene is ackA

Identifier: 195977275

GI number: 195977275

Start: 134514

End: 135716

Strand: Direct

Name: ackA

Synonym: Sez_0124

Alternate gene names: 195977275

Gene position: 134514-135716 (Clockwise)

Preceding gene: 195977274

Following gene: 195977277

Centisome position: 6.65

GC content: 43.23

Gene sequence:

>1203_bases
ATGTCAAAGACAATTGCAATTAATGCAGGAAGCTCCAGCTTAAAATGGCAGCTATATCAAATGCCAGAAGAAAAGGTATT
AGCACAGGGAATTATCGAACGTATCGGTCTGACAGACTCAATCTCTACTGTTAAATACGATGGCAAAAAAGAAGAGCATA
TTCTTGATATTCCCGATCATACAGAGGCTGTTAAGCGTTTGTTGAATGATTTGATTCATTTTGGCATTATTGATACGTAT
GACGAGATCACAGGTGTTGGGCACCGTATTGTTGCAGGTGGTGAATACTTCAAGGAATCTGTGGTGGTTGATGATAAGGT
TGTTGAGCAGGTTGAGGAGTTAGCAGCCTTGGCACCCTTGCACAATCCGGGGGCAGCAGCAGGTATTCGTGCCTTTCGTA
AGATTTTGCCGGACATTACGAGTGTTTGTGTGTTTGATACTTCGTTTCATACCACAATGCAAAAGCATACCTACCTTTAT
CCAATTCCTCAAAAATATTATACAGATTATAAGGTTCGCAAGTACGGGGCGCATGGGACTAGCCATAAATATGTTGCTGA
GGAAGCAGCAAAAATGCTGGGTCGCCCTCTAGACGAATTAAAGCTCATTACAGCCCATGTTGGTAATGGTGTGTCTATCA
CAGCCAATTATCATGGTCAGTCAGTGGATACCTCTATGGGCTTTACACCGCTTGCTGGTCCGATGATGGGGACACGCTCT
GGTGATATTGATCCTGCTATTATTCCTTATTTGATTGCTCAGGATCCTGAGCTAAAGGACGCAGCAGATGTGGTCAATAT
GCTTAACAAGCAATCTGGATTAGGTGGTGTGTCTGGTATTTCCAGTGATATGCGTGATATTGAGGCTGGCTTGCAGGCTA
ACAATCCAGATGCCGTCTTAGCTTACAATATCTTTATTGATCGTATCAAGAAATTTATCGGACAATACTTTGCGGTATTA
AATGGTGCAGATGCCTTGGTCTTTACCGCAGGTATGGGTGAGAATGCGCCCTTGATGCGCCAAGATGTCGTCAATGGCTT
GTCATGGTTTGGTATGGAAATTGATCCAGAGAAAAATGTGTTTGGTTATCGTGGTGATATCTCAACAGCAGCTTCAAAGG
TGAAGGTGCTTGTCATCTCAACCGATGAAGAGCTGTGCATTGCGCGTGACGTTGAACGCTTGAAAAAAACAGTATCATCA
TAG

Upstream 100 bases:

>100_bases
TGGAAAACTTCAAAAATTGGAAACGGGATAATGTTATTTAAGAGAATTTTTTGATATAATAGAAAAGCATTGCAGAAAAC
GATTTCAAGAGAGGTAAAAC

Downstream 100 bases:

>100_bases
CCTGATGATATACTCTTAGCTCAGCTAATCAGTTTTAAAGAGTCCAAAAAATACTTTTAATTGGTACCGACCTCCAAAAG
TTAGACTTAAAGGTTTAACG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVKYDGKKEEHILDIPDHTEAVKRLLNDLIHFGIIDTY
DEITGVGHRIVAGGEYFKESVVVDDKVVEQVEELAALAPLHNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLY
PIPQKYYTDYKVRKYGAHGTSHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSVDTSMGFTPLAGPMMGTRS
GDIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGISSDMRDIEAGLQANNPDAVLAYNIFIDRIKKFIGQYFAVL
NGADALVFTAGMGENAPLMRQDVVNGLSWFGMEIDPEKNVFGYRGDISTAASKVKVLVISTDEELCIARDVERLKKTVSS

Sequences:

>Translated_400_residues
MSKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVKYDGKKEEHILDIPDHTEAVKRLLNDLIHFGIIDTY
DEITGVGHRIVAGGEYFKESVVVDDKVVEQVEELAALAPLHNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLY
PIPQKYYTDYKVRKYGAHGTSHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSVDTSMGFTPLAGPMMGTRS
GDIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGISSDMRDIEAGLQANNPDAVLAYNIFIDRIKKFIGQYFAVL
NGADALVFTAGMGENAPLMRQDVVNGLSWFGMEIDPEKNVFGYRGDISTAASKVKVLVISTDEELCIARDVERLKKTVSS
>Mature_399_residues
SKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVKYDGKKEEHILDIPDHTEAVKRLLNDLIHFGIIDTYD
EITGVGHRIVAGGEYFKESVVVDDKVVEQVEELAALAPLHNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLYP
IPQKYYTDYKVRKYGAHGTSHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSVDTSMGFTPLAGPMMGTRSG
DIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGISSDMRDIEAGLQANNPDAVLAYNIFIDRIKKFIGQYFAVLN
GADALVFTAGMGENAPLMRQDVVNGLSWFGMEIDPEKNVFGYRGDISTAASKVKVLVISTDEELCIARDVERLKKTVSS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=42.4623115577889, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI145693184, Length=407, Percent_Identity=38.8206388206388, Blast_Score=274, Evalue=7e-75,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_STREM (B4U063)

Other databases:

- EMBL:   CP001129
- RefSeq:   YP_002122519.1
- EnsemblBacteria:   EBSTRT00000009321
- GeneID:   6762257
- GenomeReviews:   CP001129_GR
- KEGG:   sez:Sez_0124
- GeneTree:   EBGT00050000027501
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- ProtClustDB:   PRK00180
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43728; Mature: 43597

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVKYDGKKEEHILDIPDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCH
TEAVKRLLNDLIHFGIIDTYDEITGVGHRIVAGGEYFKESVVVDDKVVEQVEELAALAPL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECHHHHHHHHEECHHHHHHHHHHHHHCCC
HNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLYPIPQKYYTDYKVRKYGAHGT
CCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCEEEEECCHHHCCCCCEEEECCCCC
SHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSVDTSMGFTPLAGPMMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GDIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGISSDMRDIEAGLQANNPDAVL
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
AYNIFIDRIKKFIGQYFAVLNGADALVFTAGMGENAPLMRQDVVNGLSWFGMEIDPEKNV
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEECCCCCC
FGYRGDISTAASKVKVLVISTDEELCIARDVERLKKTVSS
CEECCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVKYDGKKEEHILDIPDH
CCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCH
TEAVKRLLNDLIHFGIIDTYDEITGVGHRIVAGGEYFKESVVVDDKVVEQVEELAALAPL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECHHHHHHHHEECHHHHHHHHHHHHHCCC
HNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLYPIPQKYYTDYKVRKYGAHGT
CCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCEEEEECCHHHCCCCCEEEECCCCC
SHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSVDTSMGFTPLAGPMMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GDIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGISSDMRDIEAGLQANNPDAVL
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
AYNIFIDRIKKFIGQYFAVLNGADALVFTAGMGENAPLMRQDVVNGLSWFGMEIDPEKNV
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEECCCCCC
FGYRGDISTAASKVKVLVISTDEELCIARDVERLKKTVSS
CEECCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA