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The map label for this gene is idi [C]

Identifier: 195977252

GI number: 195977252

Start: 106820

End: 107344

Strand: Direct

Name: idi [C]

Synonym: Sez_0101

Alternate gene names: 195977252

Gene position: 106820-107344 (Clockwise)

Preceding gene: 195977251

Following gene: 195977253

Centisome position: 5.28

GC content: 46.29

Gene sequence:

>525_bases
GTGGTTGAGTATTGGGACGTTTATACGATGAATCGTCTTAAGACCGGTAGGGTCATGGAGCGTGGGGCTGCTTTTGTAGA
GGGGGCTTATCATTTAGTAGTTCACGCCTGTGTCTTTAATGACAAGGGAGAAATGCTGATTCAGCAGCGGCAAAAGGATA
AAGAGGGCTGGCCCGGCTATTGGGATATAACGGTAGGCGGCAGCGCTCTTGCAGGTGAGACGTCACAAGAAGCAGTGATG
AGAGAGCTGAAGGAGGAGCTAGGACTTGCTCTTGATTTGGCTGGTGTAAGGCCACACTTTTCAATCACCTTTGATAATGG
CTTTGACGATACTTTTTTAGTTTTGCAGGCTGTTGATGTAACGAAGCTTGTTCTTCAGACAGAGGAGGTACAGGCTGTTC
GATGGGCTAGTCGTGATGAGATTTTAGCCATGATTGATGCTGGTATTTTTATTCCCTACTTTAAGAGCAAGATTGATATG
TGCTTTGAGTTAGTGGGGCAATATGGAGCGATCAAGTGGTCATAG

Upstream 100 bases:

>100_bases
ATTGTTGTTCTGTCTGGATCTGGTTGTTTTGGATATGCTAATTGTGATATGATTAATATATTGGATCTGGTAATAGCTGT
GTCAAAGGAGGTGCGTTTGT

Downstream 100 bases:

>100_bases
AGACTGCTGCTGTCTTAGCTTTGCTTGCTATGCTAGTAGTTGTCGTTTTTGGCTGCTGATCTGGTAAAGCCTCACGCTAG
TAGGGACAAGTGGTTGGCTA

Product: putative nudix hydrolase

Products: Dimethylallyl diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MVEYWDVYTMNRLKTGRVMERGAAFVEGAYHLVVHACVFNDKGEMLIQQRQKDKEGWPGYWDITVGGSALAGETSQEAVM
RELKEELGLALDLAGVRPHFSITFDNGFDDTFLVLQAVDVTKLVLQTEEVQAVRWASRDEILAMIDAGIFIPYFKSKIDM
CFELVGQYGAIKWS

Sequences:

>Translated_174_residues
MVEYWDVYTMNRLKTGRVMERGAAFVEGAYHLVVHACVFNDKGEMLIQQRQKDKEGWPGYWDITVGGSALAGETSQEAVM
RELKEELGLALDLAGVRPHFSITFDNGFDDTFLVLQAVDVTKLVLQTEEVQAVRWASRDEILAMIDAGIFIPYFKSKIDM
CFELVGQYGAIKWS
>Mature_174_residues
MVEYWDVYTMNRLKTGRVMERGAAFVEGAYHLVVHACVFNDKGEMLIQQRQKDKEGWPGYWDITVGGSALAGETSQEAVM
RELKEELGLALDLAGVRPHFSITFDNGFDDTFLVLQAVDVTKLVLQTEEVQAVRWASRDEILAMIDAGIFIPYFKSKIDM
CFELVGQYGAIKWS

Specific function: Catalyzes The 1,3-Allylic Rearrangement Of The Homoallylic Substrate Isopentenyl (Ipp) To Its Highly Electrophilic Allylic Isomer, Dimethylallyl Diphosphate (Dmapp). [C]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789255, Length=140, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 5.3.3.2 [C]

Molecular weight: Translated: 19694; Mature: 19694

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEYWDVYTMNRLKTGRVMERGAAFVEGAYHLVVHACVFNDKGEMLIQQRQKDKEGWPGY
CCEEECEEECCCCCCCHHHHHCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHCCCCCCCCE
WDITVGGSALAGETSQEAVMRELKEELGLALDLAGVRPHFSITFDNGFDDTFLVLQAVDV
EEEEECCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCCCHHHEEEHHHH
TKLVLQTEEVQAVRWASRDEILAMIDAGIFIPYFKSKIDMCFELVGQYGAIKWS
HHHHHHHHHHHHHHHCCHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MVEYWDVYTMNRLKTGRVMERGAAFVEGAYHLVVHACVFNDKGEMLIQQRQKDKEGWPGY
CCEEECEEECCCCCCCHHHHHCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHCCCCCCCCE
WDITVGGSALAGETSQEAVMRELKEELGLALDLAGVRPHFSITFDNGFDDTFLVLQAVDV
EEEEECCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCCCHHHEEEHHHH
TKLVLQTEEVQAVRWASRDEILAMIDAGIFIPYFKSKIDMCFELVGQYGAIKWS
HHHHHHHHHHHHHHHCCHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.036 {isopentenyl} [C]

Substrates: Isopentenyl diphosphate [C]

Specific reaction: Isopentenyl diphosphate --> Dimethylallyl diphosphate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Iodoacetamide; PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]