Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 195977184

GI number: 195977184

Start: 39223

End: 40725

Strand: Direct

Name: purH [H]

Synonym: Sez_0028

Alternate gene names: 195977184

Gene position: 39223-40725 (Clockwise)

Preceding gene: 195977183

Following gene: 195977186

Centisome position: 1.94

GC content: 44.51

Gene sequence:

>1503_bases
GTGGACCTTGCTAGGGAGCTTAAGCATCTTGGCTGGGATATTATATCAACAGGAGGAACAAGGCTTGCTCTTGAAGAGGC
TGGTGTAGTTACTGTTGCTATTGACGATGTGACAGGCTTTCCTGAAATGATGGACGGACGGGTAAAAACGCTGCACCCTC
TCATTCATGGTGGCTTACTTGCTCGTCGTGACATTGAGCACCACTTACAGGCAGCAAAGCAAAACAGGATTGAATTAATA
GACCTTGTTGTTGTTAACCTTTATCCCTTCAAAGAAACCATTTGCCGTCCAGATGTCACTTATGATGTCGCTGTTGATAT
GGTTGATATTGGTGGTCCTTCACTGCTGCGTTCAGCGGCTAAAAATCATGCTAGTGTTACGGTAGTGGTTGATCCGACAG
ATTATCCGCTCGTTTTAGGAGAGTTGGCTAGCACTGGCAGTACAAGTTACCAAACCCGTCAAAGCTTAGCAGCTAAAGCT
TTTCGCCATACTGCAGCCTATGATGCAGTGATTGCAGATTATTTTACAAGACAAGCAGGTGAGACAAAGCCTGAAAAGCT
CACGCTTACCTATGATTTGAAGCAAAGCATGCGTTACGGTGAAAATCCTCAACAGGCTGCAGATTTTTACCAAAGAGCGC
TCCCAACAACCTATTCAATTGCTTCAGCCAAGCAGCTAAATGGAAAAGAGCTATCATTCAACAATATTCGTGATGCAGAT
GCTGCGATTCGTATTATTCGTGATTTTAAGGAGCGTCCAACCGTTGTAGCACTCAAGCATATGAATCCATGTGGTATTGG
TCAGGCAGATGATATTGAGACAGCTTGGGATTTTGCTTATGCAGCTGATCCGGTGTCTATCTTTGGTGGTATTGTTGTCC
TAAATCGTGAGGTGGATCTAGCGACAGCGGAGAAGCTTCATGCTATTTTTCTTGAATTGATCATTGCACCAGGCTATTCC
AAAGAAGCGCTAGCTGTTCTCACCCATAAAAAGAAGCACTTGCGAATTCTTGAATTGCCATTTGCTGCTCAGGAGGCTAG
TGAAATAGAAGCAGAATATACAGGAGTTCTTGGTGGTCTACTAGTGCAAAATCAAGATGTTGTCACAGAGTCTCCAGCGG
ATTGGATAGTGGTGACAAAGCGTCAGCCAAATGAACAGGAAATGGCTGCTCTAGCATTTGCTTGGAAAACCATCAAATAT
GTCAAGTCTAATGCGATTGTCATTGCTAATGATCATATGACTTTGGGCGTTGGTCCAGGGCAAACAAACCGAATAGCCTC
TGTTCGTATTGCTATTGCGCAAGCAACGGGGCAGCTTGAGGGAGCAGTTCTTGCTAGCGATGCCTTCTTCCCATTTGCAG
ACAGCATTGAAGAAATTGCTGCCGCTGGTATCAAGGCTATTATCCAGCCTGGAGGCTCTATTCGTGATTCAGAATCTATT
GCAGCTGCCAATCAGCATGGTATAACCATGATTTTCACAGGTGTGAGGCATTTTAGACATTAA

Upstream 100 bases:

>100_bases
GTTTGATGATGGCACTGCTATTGAGGAAAATAGACTTTTCTAAGGAGAACTTAAAATGACTAAACGCGCCTTAATTAGCG
TATCAGATAAAAGAGGGATT

Downstream 100 bases:

>100_bases
TAAAGTAAAAAGAGACTGGAGCCAAAAGAGTTGCACTTTAGAAATTCTTGGTTAATATACTTTATTACCTAGTTGGATTC
ATTAAAAAGCGAACAAGTCT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MDLARELKHLGWDIISTGGTRLALEEAGVVTVAIDDVTGFPEMMDGRVKTLHPLIHGGLLARRDIEHHLQAAKQNRIELI
DLVVVNLYPFKETICRPDVTYDVAVDMVDIGGPSLLRSAAKNHASVTVVVDPTDYPLVLGELASTGSTSYQTRQSLAAKA
FRHTAAYDAVIADYFTRQAGETKPEKLTLTYDLKQSMRYGENPQQAADFYQRALPTTYSIASAKQLNGKELSFNNIRDAD
AAIRIIRDFKERPTVVALKHMNPCGIGQADDIETAWDFAYAADPVSIFGGIVVLNREVDLATAEKLHAIFLELIIAPGYS
KEALAVLTHKKKHLRILELPFAAQEASEIEAEYTGVLGGLLVQNQDVVTESPADWIVVTKRQPNEQEMAALAFAWKTIKY
VKSNAIVIANDHMTLGVGPGQTNRIASVRIAIAQATGQLEGAVLASDAFFPFADSIEEIAAAGIKAIIQPGGSIRDSESI
AAANQHGITMIFTGVRHFRH

Sequences:

>Translated_500_residues
MDLARELKHLGWDIISTGGTRLALEEAGVVTVAIDDVTGFPEMMDGRVKTLHPLIHGGLLARRDIEHHLQAAKQNRIELI
DLVVVNLYPFKETICRPDVTYDVAVDMVDIGGPSLLRSAAKNHASVTVVVDPTDYPLVLGELASTGSTSYQTRQSLAAKA
FRHTAAYDAVIADYFTRQAGETKPEKLTLTYDLKQSMRYGENPQQAADFYQRALPTTYSIASAKQLNGKELSFNNIRDAD
AAIRIIRDFKERPTVVALKHMNPCGIGQADDIETAWDFAYAADPVSIFGGIVVLNREVDLATAEKLHAIFLELIIAPGYS
KEALAVLTHKKKHLRILELPFAAQEASEIEAEYTGVLGGLLVQNQDVVTESPADWIVVTKRQPNEQEMAALAFAWKTIKY
VKSNAIVIANDHMTLGVGPGQTNRIASVRIAIAQATGQLEGAVLASDAFFPFADSIEEIAAAGIKAIIQPGGSIRDSESI
AAANQHGITMIFTGVRHFRH
>Mature_500_residues
MDLARELKHLGWDIISTGGTRLALEEAGVVTVAIDDVTGFPEMMDGRVKTLHPLIHGGLLARRDIEHHLQAAKQNRIELI
DLVVVNLYPFKETICRPDVTYDVAVDMVDIGGPSLLRSAAKNHASVTVVVDPTDYPLVLGELASTGSTSYQTRQSLAAKA
FRHTAAYDAVIADYFTRQAGETKPEKLTLTYDLKQSMRYGENPQQAADFYQRALPTTYSIASAKQLNGKELSFNNIRDAD
AAIRIIRDFKERPTVVALKHMNPCGIGQADDIETAWDFAYAADPVSIFGGIVVLNREVDLATAEKLHAIFLELIIAPGYS
KEALAVLTHKKKHLRILELPFAAQEASEIEAEYTGVLGGLLVQNQDVVTESPADWIVVTKRQPNEQEMAALAFAWKTIKY
VKSNAIVIANDHMTLGVGPGQTNRIASVRIAIAQATGQLEGAVLASDAFFPFADSIEEIAAAGIKAIIQPGGSIRDSESI
AAANQHGITMIFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=462, Percent_Identity=37.2294372294372, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1790439, Length=515, Percent_Identity=46.7961165048544, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71985564, Length=456, Percent_Identity=33.1140350877193, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71985574, Length=301, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=461, Percent_Identity=37.7440347071584, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6323056, Length=459, Percent_Identity=34.640522875817, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24649832, Length=459, Percent_Identity=37.037037037037, Blast_Score=252, Evalue=5e-67,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54505; Mature: 54505

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLARELKHLGWDIISTGGTRLALEEAGVVTVAIDDVTGFPEMMDGRVKTLHPLIHGGLL
CCHHHHHHHCCCEEEECCCCEEEEECCCEEEEEECCCCCCHHHHCCCHHHHHHHHHCCHH
ARRDIEHHLQAAKQNRIELIDLVVVNLYPFKETICRPDVTYDVAVDMVDIGGPSLLRSAA
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHCCCCCEEEEEEEEEECCCHHHHHHHH
KNHASVTVVVDPTDYPLVLGELASTGSTSYQTRQSLAAKAFRHTAAYDAVIADYFTRQAG
CCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETKPEKLTLTYDLKQSMRYGENPQQAADFYQRALPTTYSIASAKQLNGKELSFNNIRDAD
CCCCCEEEEEEEHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHCCCCEECCCCCCCHH
AAIRIIRDFKERPTVVALKHMNPCGIGQADDIETAWDFAYAADPVSIFGGIVVLNREVDL
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCEEEEECCCCH
ATAEKLHAIFLELIIAPGYSKEALAVLTHKKKHLRILELPFAAQEASEIEAEYTGVLGGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LVQNQDVVTESPADWIVVTKRQPNEQEMAALAFAWKTIKYVKSNAIVIANDHMTLGVGPG
EECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCC
QTNRIASVRIAIAQATGQLEGAVLASDAFFPFADSIEEIAAAGIKAIIQPGGSIRDSESI
CCCCEEEEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AAANQHGITMIFTGVRHFRH
HHCCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MDLARELKHLGWDIISTGGTRLALEEAGVVTVAIDDVTGFPEMMDGRVKTLHPLIHGGLL
CCHHHHHHHCCCEEEECCCCEEEEECCCEEEEEECCCCCCHHHHCCCHHHHHHHHHCCHH
ARRDIEHHLQAAKQNRIELIDLVVVNLYPFKETICRPDVTYDVAVDMVDIGGPSLLRSAA
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHCCCCCEEEEEEEEEECCCHHHHHHHH
KNHASVTVVVDPTDYPLVLGELASTGSTSYQTRQSLAAKAFRHTAAYDAVIADYFTRQAG
CCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETKPEKLTLTYDLKQSMRYGENPQQAADFYQRALPTTYSIASAKQLNGKELSFNNIRDAD
CCCCCEEEEEEEHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHCCCCEECCCCCCCHH
AAIRIIRDFKERPTVVALKHMNPCGIGQADDIETAWDFAYAADPVSIFGGIVVLNREVDL
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCEEEEECCCCH
ATAEKLHAIFLELIIAPGYSKEALAVLTHKKKHLRILELPFAAQEASEIEAEYTGVLGGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LVQNQDVVTESPADWIVVTKRQPNEQEMAALAFAWKTIKYVKSNAIVIANDHMTLGVGPG
EECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCC
QTNRIASVRIAIAQATGQLEGAVLASDAFFPFADSIEEIAAAGIKAIIQPGGSIRDSESI
CCCCEEEEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AAANQHGITMIFTGVRHFRH
HHCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA