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The map label for this gene is purN [H]

Identifier: 195977183

GI number: 195977183

Start: 38474

End: 39031

Strand: Direct

Name: purN [H]

Synonym: Sez_0027

Alternate gene names: 195977183

Gene position: 38474-39031 (Clockwise)

Preceding gene: 195977182

Following gene: 195977184

Centisome position: 1.9

GC content: 42.47

Gene sequence:

>558_bases
ATGACAAGAATTGCCGTTTTTGCTTCTGGTAATGGCTCCAATTTTCAAACGATTGCAGAACAGTTTCCGGTTGCCTTTGT
TTTTTCTGATCATTGCGATGCTCATGTTTTAAGTAGAGCCTGTGCTTTGGGTGTTCTGTCCTACAGCTTTGAACTAAAGG
ACTTTGAGAATAAGCAAGCCTATGAGCAGACTCTTGTGGCATTGCTGCAGCGGCATCAGATTGATCTCATCGTCCTTGCC
GGCTATATGAAAATTGTGAGTACAACCTTACTTGATGCTTACGGAGGAAAAATCATCAACATTCACCCAGCTTATTTGCC
AGAATTTCCAGGAGCACATGGTATTTTAGATGCTTGGCAAGCAGGTGTTAGTCAGTCAGGAGTGACTGTTCATTGGGTTG
ATAGTGGTATTGATACAGGAAAAATCATCAAACAGGTACGAGTACCACGTTTGAGTGATGATACTCTTGAAAGCTTTGAA
GCCAGAATTCATGAAGCAGAATACCAATTGTATCCAGAGGTACTGGATAGTCTTGGAGTAGAGCGGCTTAACCGCTGA

Upstream 100 bases:

>100_bases
AGCCTGAGCACGTTGAGCAGGTGAAGGCCTGTCTTGATGAGCCTGTGTATGACATTGGTCGCGTTGTGACCAGAACAGTT
GCTAGTGTGGTGATGAAGCC

Downstream 100 bases:

>100_bases
TTGTGTAAGATTAATAACTATTTTCCCTATTGTGGCATTTCGTTTATCATAGCAGCTGCGCTAATGGTGGCTAACAAAGG
CTAGAAGACTTGTTTGATGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTRIAVFASGNGSNFQTIAEQFPVAFVFSDHCDAHVLSRACALGVLSYSFELKDFENKQAYEQTLVALLQRHQIDLIVLA
GYMKIVSTTLLDAYGGKIINIHPAYLPEFPGAHGILDAWQAGVSQSGVTVHWVDSGIDTGKIIKQVRVPRLSDDTLESFE
ARIHEAEYQLYPEVLDSLGVERLNR

Sequences:

>Translated_185_residues
MTRIAVFASGNGSNFQTIAEQFPVAFVFSDHCDAHVLSRACALGVLSYSFELKDFENKQAYEQTLVALLQRHQIDLIVLA
GYMKIVSTTLLDAYGGKIINIHPAYLPEFPGAHGILDAWQAGVSQSGVTVHWVDSGIDTGKIIKQVRVPRLSDDTLESFE
ARIHEAEYQLYPEVLDSLGVERLNR
>Mature_184_residues
TRIAVFASGNGSNFQTIAEQFPVAFVFSDHCDAHVLSRACALGVLSYSFELKDFENKQAYEQTLVALLQRHQIDLIVLAG
YMKIVSTTLLDAYGGKIINIHPAYLPEFPGAHGILDAWQAGVSQSGVTVHWVDSGIDTGKIIKQVRVPRLSDDTLESFEA
RIHEAEYQLYPEVLDSLGVERLNR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=34.0425531914894, Blast_Score=123, Evalue=6e-30,
Organism=Escherichia coli, GI1787483, Length=98, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=28.5, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=38.9189189189189, Blast_Score=136, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20524; Mature: 20392

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIAVFASGNGSNFQTIAEQFPVAFVFSDHCDAHVLSRACALGVLSYSFELKDFENKQA
CCEEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHH
YEQTLVALLQRHQIDLIVLAGYMKIVSTTLLDAYGGKIINIHPAYLPEFPGAHGILDAWQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHH
AGVSQSGVTVHWVDSGIDTGKIIKQVRVPRLSDDTLESFEARIHEAEYQLYPEVLDSLGV
HCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLNR
HHHCC
>Mature Secondary Structure 
TRIAVFASGNGSNFQTIAEQFPVAFVFSDHCDAHVLSRACALGVLSYSFELKDFENKQA
CEEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHH
YEQTLVALLQRHQIDLIVLAGYMKIVSTTLLDAYGGKIINIHPAYLPEFPGAHGILDAWQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHH
AGVSQSGVTVHWVDSGIDTGKIIKQVRVPRLSDDTLESFEARIHEAEYQLYPEVLDSLGV
HCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERLNR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]