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The map label for this gene is purF [H]

Identifier: 195977181

GI number: 195977181

Start: 35941

End: 37398

Strand: Direct

Name: purF [H]

Synonym: Sez_0025

Alternate gene names: 195977181

Gene position: 35941-37398 (Clockwise)

Preceding gene: 195977180

Following gene: 195977182

Centisome position: 1.78

GC content: 44.86

Gene sequence:

>1458_bases
ATGACATATGACGTAAAATCTCTTAATGAGGAGTGTGGTGTTTTTGGGATTTGGGGACACCCACATGCCGCTCAAGTGAC
TTATTTTGGGCTTCATAGCCTACAGCACCGCGGCCAAGAGGGAGCAGGTATTATTTCAAATGATAATGGGGAGCTTCATC
AGCATCGTGATGTAGGTCTCTTGTCAGAGGTGTTTAAGGATTCATCTGATCTGGACAAGCTAAAGGGACATGCTGCCATT
GGTCATGTGCGGTATGCGACTGCTGGGACGGCTTCCATTAATAATATTCAGCCCTTTTTATATCGTTTTACAGACGCTCA
ATTTGGCTTGTGCCACAATGGTAATTTGACCAATGCTATGTCATTGAAAAAAGCGTTAGAGAGTGAGGGAGCTATTTTTA
ATGCCTCTTCTGATACAGAAATTCTAATGCATTTGATTCGTCGTAGTCATCACCCAGAATTTTTAGGAAAGGTAAAAGAG
GCACTCAACACTGTTAAAGGGGGCTTTGCTTACCTGCTGGTGACAGAGAACCAGCTGATAGCAGCGCTTGATCCAAACGG
CTTTAGGCCGTTGTCTATAGGCCGTATGAAAAATGGGGCTTGGGTCGTCTCAAGTGAGACCTGTGCCTTTGAAGTGGTTG
GTGCTGAGTGGGTTCGCGATGTGATGCCAGGTGAGGTCATTATCATTGATGATCAGGGGATCACTTATGACTGCTATACG
ACTACTACGCAGCTGGCTATCTGCTCTATGGAATATATTTACTTTGCGCGTCCTGATTCAACCATTGCTGGAGTCAATGT
TCACACCGCTCGCAAGCGAATGGGAAAGAGACTGGCTCAGGAATTTCAGCATGAGGCTGATATTGTGGTTGGTGTGCCAA
ATTCCTCATTATCAGCAGCGATGGGCTTTGCAGAAGCATCAGGCCTGCCAAATGAAATGGGATTGGTCAAGAATCAATAT
ACCCAACGAACCTTTATCCAACCAACTCAAGAGCTTCGTGAGCAGGGAGTTCGAATGAAGCTTTCAGCTGTTTCAGGTGT
TGTAAAGGGTAAAAGGGTTGTGATGGTAGATGATTCTATTGTCAGAGGGACAACCTCACGACGCATTGTTAATTTGCTGC
GTGAAGCAGGAGCTACAGAGGTTCATGTAGCAATTGGTAGCCCTGAGCTCAAATACCCTTGCTTTTATGGGATTGATATT
CAGACAAGACGGGAGCTGATTTCAGCTAATCATACTGCTGCTGAGATATGTGATATTATCGGTGCTGATAGCCTGACTTA
TTTGTCACTTGATGGGCTTATCGAGGCTATTGGACTTGATACAAAAGCACCAAATGGTGGGCTTTGTGTGGCTTATTTTG
ACGGTCAGTATCCAACGCCGCTTTATGATTATGAGGAGGCTTATCTCAAGAGCTTAGAAGAAAAGGTAAGCTTTCACATT
GATCAGGTGACGAAGTAG

Upstream 100 bases:

>100_bases
AATAGCTAAGATAGCTGCTATTATTGCTCAGCCTAGCTGCTAGTTCTAGCTTAGTTAATGACACAATCATAATTAAAAGG
GCATTAGCCCAGAGGTAAAG

Downstream 100 bases:

>100_bases
CGCTTTATGGCTAACATAGAATAAATGATCTAGGCAGCTAAAATAAGGAGGAGAAAATGTCTAAAAATGCTTATGCTCAA
TCAGGTGTCGATGTTGAAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTYDVKSLNEECGVFGIWGHPHAAQVTYFGLHSLQHRGQEGAGIISNDNGELHQHRDVGLLSEVFKDSSDLDKLKGHAAI
GHVRYATAGTASINNIQPFLYRFTDAQFGLCHNGNLTNAMSLKKALESEGAIFNASSDTEILMHLIRRSHHPEFLGKVKE
ALNTVKGGFAYLLVTENQLIAALDPNGFRPLSIGRMKNGAWVVSSETCAFEVVGAEWVRDVMPGEVIIIDDQGITYDCYT
TTTQLAICSMEYIYFARPDSTIAGVNVHTARKRMGKRLAQEFQHEADIVVGVPNSSLSAAMGFAEASGLPNEMGLVKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVNLLREAGATEVHVAIGSPELKYPCFYGIDI
QTRRELISANHTAAEICDIIGADSLTYLSLDGLIEAIGLDTKAPNGGLCVAYFDGQYPTPLYDYEEAYLKSLEEKVSFHI
DQVTK

Sequences:

>Translated_485_residues
MTYDVKSLNEECGVFGIWGHPHAAQVTYFGLHSLQHRGQEGAGIISNDNGELHQHRDVGLLSEVFKDSSDLDKLKGHAAI
GHVRYATAGTASINNIQPFLYRFTDAQFGLCHNGNLTNAMSLKKALESEGAIFNASSDTEILMHLIRRSHHPEFLGKVKE
ALNTVKGGFAYLLVTENQLIAALDPNGFRPLSIGRMKNGAWVVSSETCAFEVVGAEWVRDVMPGEVIIIDDQGITYDCYT
TTTQLAICSMEYIYFARPDSTIAGVNVHTARKRMGKRLAQEFQHEADIVVGVPNSSLSAAMGFAEASGLPNEMGLVKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVNLLREAGATEVHVAIGSPELKYPCFYGIDI
QTRRELISANHTAAEICDIIGADSLTYLSLDGLIEAIGLDTKAPNGGLCVAYFDGQYPTPLYDYEEAYLKSLEEKVSFHI
DQVTK
>Mature_484_residues
TYDVKSLNEECGVFGIWGHPHAAQVTYFGLHSLQHRGQEGAGIISNDNGELHQHRDVGLLSEVFKDSSDLDKLKGHAAIG
HVRYATAGTASINNIQPFLYRFTDAQFGLCHNGNLTNAMSLKKALESEGAIFNASSDTEILMHLIRRSHHPEFLGKVKEA
LNTVKGGFAYLLVTENQLIAALDPNGFRPLSIGRMKNGAWVVSSETCAFEVVGAEWVRDVMPGEVIIIDDQGITYDCYTT
TTQLAICSMEYIYFARPDSTIAGVNVHTARKRMGKRLAQEFQHEADIVVGVPNSSLSAAMGFAEASGLPNEMGLVKNQYT
QRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVNLLREAGATEVHVAIGSPELKYPCFYGIDIQ
TRRELISANHTAAEICDIIGADSLTYLSLDGLIEAIGLDTKAPNGGLCVAYFDGQYPTPLYDYEEAYLKSLEEKVSFHID
QVTK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=466, Percent_Identity=39.0557939914163, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI4826742, Length=233, Percent_Identity=27.4678111587983, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788651, Length=487, Percent_Identity=37.9876796714579, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI1790167, Length=202, Percent_Identity=24.2574257425743, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=38.5416666666667, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17532899, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532897, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=34.1948310139165, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=38.5714285714286, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=38.4, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24659604, Length=459, Percent_Identity=39.2156862745098, Blast_Score=325, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53242; Mature: 53111

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYDVKSLNEECGVFGIWGHPHAAQVTYFGLHSLQHRGQEGAGIISNDNGELHQHRDVGL
CCCCHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCCHHCCCCHH
LSEVFKDSSDLDKLKGHAAIGHVRYATAGTASINNIQPFLYRFTDAQFGLCHNGNLTNAM
HHHHHCCCCCHHHHCCCCEECEEEEECCCCCCCCCCCHHHEEECCCCEEEEECCCCCHHH
SLKKALESEGAIFNASSDTEILMHLIRRSHHPEFLGKVKEALNTVKGGFAYLLVTENQLI
HHHHHHHHCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEE
AALDPNGFRPLSIGRMKNGAWVVSSETCAFEVVGAEWVRDVMPGEVIIIDDQGITYDCYT
EEECCCCCCEEEECEECCCEEEEECCCEEEEEECHHHHHHCCCCCEEEECCCCCEEEEEC
TTTQLAICSMEYIYFARPDSTIAGVNVHTARKRMGKRLAQEFQHEADIVVGVPNSSLSAA
CHHHHEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEASGLPNEMGLVKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSI
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVNLLREAGATEVHVAIGSPELKYPCFYGIDIQTRRELISANHTAAEICDII
HCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHH
GADSLTYLSLDGLIEAIGLDTKAPNGGLCVAYFDGQYPTPLYDYEEAYLKSLEEKVSFHI
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
DQVTK
HHCCC
>Mature Secondary Structure 
TYDVKSLNEECGVFGIWGHPHAAQVTYFGLHSLQHRGQEGAGIISNDNGELHQHRDVGL
CCCHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCCHHCCCCHH
LSEVFKDSSDLDKLKGHAAIGHVRYATAGTASINNIQPFLYRFTDAQFGLCHNGNLTNAM
HHHHHCCCCCHHHHCCCCEECEEEEECCCCCCCCCCCHHHEEECCCCEEEEECCCCCHHH
SLKKALESEGAIFNASSDTEILMHLIRRSHHPEFLGKVKEALNTVKGGFAYLLVTENQLI
HHHHHHHHCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEE
AALDPNGFRPLSIGRMKNGAWVVSSETCAFEVVGAEWVRDVMPGEVIIIDDQGITYDCYT
EEECCCCCCEEEECEECCCEEEEECCCEEEEEECHHHHHHCCCCCEEEECCCCCEEEEEC
TTTQLAICSMEYIYFARPDSTIAGVNVHTARKRMGKRLAQEFQHEADIVVGVPNSSLSAA
CHHHHEEEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEASGLPNEMGLVKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSI
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVNLLREAGATEVHVAIGSPELKYPCFYGIDIQTRRELISANHTAAEICDII
HCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHH
GADSLTYLSLDGLIEAIGLDTKAPNGGLCVAYFDGQYPTPLYDYEEAYLKSLEEKVSFHI
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
DQVTK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA