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The map label for this gene is trpE [H]

Identifier: 195952930

GI number: 195952930

Start: 492076

End: 493566

Strand: Direct

Name: trpE [H]

Synonym: HY04AAS1_0555

Alternate gene names: 195952930

Gene position: 492076-493566 (Clockwise)

Preceding gene: 195952922

Following gene: 195952931

Centisome position: 31.55

GC content: 31.86

Gene sequence:

>1491_bases
ATGAAAGTAAATATAGCTAAAGATGATTTTTTAAGCCTTTGTAAAGATTACAATACTATCCCTTTATATACCGAGATATT
GGCTGATTCTGAAACTCCTTTTTCTATATTTTATAAGCTAAAAGACGAAGAGAGTCAAAATGTTTTATTAGAAAGCGCCG
AAACGAAAGAAAACTGGGGAAGATACTCTTTTATATGTAAGGGTAAAAATTTACGTTTTTATCAAAAAGATGATACTTAT
TTTATAAAAGATGGCGATACGATAACCAAAGGAACGTCAAAGCCATTAGAAGCTTTAGAAAATCTTTATAAAAGCTTTAA
ATTTTATGAGGATAAGACACTTGGAAAATTCTGTGGTGGTTTTGTTGGTTATATAAGCTATGATATAGTAAAGCACTATC
ATCCTATTGAGAAATATACAAAAGCACAAAAATCTGGTTTAGACATACCAGACATAGATATGATATTTACCGATTTTTTA
ATAGTATTTGATAATTTAAAAGCCACTATTAAAATTATAAAACTTATAAGGGTAAGAAATCCTAAAGAAGATTACGAAAA
AGCTTTAAAAGACATAGAAGATATAATTATTAAATTAAAAAATAGCAAAGGTATTTCGTTTCTTGATATAAACAATAAAG
AACCCGATTTAAAAGAATGGTCTTCAAATATGACCAAAGAAGAATTTATTAACATAGTTAACATATGCAAAAACTATATA
GAAAACGGCGATGTGATACAAGTAGTACCTTCTCAAAGGTTTAACAAAAAAATACATACAAATCCTATAAACATATACAG
GGCGCTTAGGTATTTAAACCCGTCTCCATACATGTATTTTTTAGATTTTGATAATGTAAAAATTATAGGTTCTTCTCCAG
AAACGTTGGTTAAAATCCAAGACGATACGGTAGAAATAAGACCAATAGCCGGGACTATAAAACGTGGCAAAACCCAAGAA
GAAGATGAAGAGCTTGCAAAAAAACTTCTAAGCGATGAAAAAGAAATAGCAGAACACGTCATGCTTGTTGATTTGGCAAG
AAACGATATCGGTGCCATAGCAGAGCCTGGAAGTGTTTATGTGGATAACATGATGCATATAGAAAAATTTTCTCATGTCA
TACATATAGTCTCAAATGTATACGGGAAAAAAGCAAAGCAACATTCTATCTTTGAAGTAATCAAACATGCGTTACCAGCC
GGCACTCTATCTGGAGCTCCAAAGGTAAGGGCTATGCAGATTATAGAAGAGTTAGAGCCTACAAAAAGAAATGTATACGG
TGGCGCTATTGGTTATATAAGCCTAAATCAAAACATAGATTTTGCTATCGCTATAAGAACAGCTACAATATTGGGCAATG
ATCTTTATGTGCAAGCAGGGGCTGGTATAGTAGCAGACTCAATACCAGAAAAGGAATATTTAGAATCTTTATCAAAAGCT
TCAAGTGTTATGAAAGCTGTAAGCGTTGCAGAAGCTTCTCGTGAACTTTGA

Upstream 100 bases:

>100_bases
TCTATGCCTTGGCCTATCCTATTTGGCCCGCTTCCTAATATAATAACTTTCTTCATCAATATAATAATATATAATAAAAA
CCCATGTTATAATAAAAAAA

Downstream 100 bases:

>100_bases
GCAGTTTATACAAGACAAAAATATTTGTTTTTTAGCAAAGGGTTGGCGAGGTGAAATATATACGTATGAAGATAATAAAG
AGCTTTTTTGTATAAAAAAA

Product: Anthranilate synthase

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKVNIAKDDFLSLCKDYNTIPLYTEILADSETPFSIFYKLKDEESQNVLLESAETKENWGRYSFICKGKNLRFYQKDDTY
FIKDGDTITKGTSKPLEALENLYKSFKFYEDKTLGKFCGGFVGYISYDIVKHYHPIEKYTKAQKSGLDIPDIDMIFTDFL
IVFDNLKATIKIIKLIRVRNPKEDYEKALKDIEDIIIKLKNSKGISFLDINNKEPDLKEWSSNMTKEEFINIVNICKNYI
ENGDVIQVVPSQRFNKKIHTNPINIYRALRYLNPSPYMYFLDFDNVKIIGSSPETLVKIQDDTVEIRPIAGTIKRGKTQE
EDEELAKKLLSDEKEIAEHVMLVDLARNDIGAIAEPGSVYVDNMMHIEKFSHVIHIVSNVYGKKAKQHSIFEVIKHALPA
GTLSGAPKVRAMQIIEELEPTKRNVYGGAIGYISLNQNIDFAIAIRTATILGNDLYVQAGAGIVADSIPEKEYLESLSKA
SSVMKAVSVAEASREL

Sequences:

>Translated_496_residues
MKVNIAKDDFLSLCKDYNTIPLYTEILADSETPFSIFYKLKDEESQNVLLESAETKENWGRYSFICKGKNLRFYQKDDTY
FIKDGDTITKGTSKPLEALENLYKSFKFYEDKTLGKFCGGFVGYISYDIVKHYHPIEKYTKAQKSGLDIPDIDMIFTDFL
IVFDNLKATIKIIKLIRVRNPKEDYEKALKDIEDIIIKLKNSKGISFLDINNKEPDLKEWSSNMTKEEFINIVNICKNYI
ENGDVIQVVPSQRFNKKIHTNPINIYRALRYLNPSPYMYFLDFDNVKIIGSSPETLVKIQDDTVEIRPIAGTIKRGKTQE
EDEELAKKLLSDEKEIAEHVMLVDLARNDIGAIAEPGSVYVDNMMHIEKFSHVIHIVSNVYGKKAKQHSIFEVIKHALPA
GTLSGAPKVRAMQIIEELEPTKRNVYGGAIGYISLNQNIDFAIAIRTATILGNDLYVQAGAGIVADSIPEKEYLESLSKA
SSVMKAVSVAEASREL
>Mature_496_residues
MKVNIAKDDFLSLCKDYNTIPLYTEILADSETPFSIFYKLKDEESQNVLLESAETKENWGRYSFICKGKNLRFYQKDDTY
FIKDGDTITKGTSKPLEALENLYKSFKFYEDKTLGKFCGGFVGYISYDIVKHYHPIEKYTKAQKSGLDIPDIDMIFTDFL
IVFDNLKATIKIIKLIRVRNPKEDYEKALKDIEDIIIKLKNSKGISFLDINNKEPDLKEWSSNMTKEEFINIVNICKNYI
ENGDVIQVVPSQRFNKKIHTNPINIYRALRYLNPSPYMYFLDFDNVKIIGSSPETLVKIQDDTVEIRPIAGTIKRGKTQE
EDEELAKKLLSDEKEIAEHVMLVDLARNDIGAIAEPGSVYVDNMMHIEKFSHVIHIVSNVYGKKAKQHSIFEVIKHALPA
GTLSGAPKVRAMQIIEELEPTKRNVYGGAIGYISLNQNIDFAIAIRTATILGNDLYVQAGAGIVADSIPEKEYLESLSKA
SSVMKAVSVAEASREL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=384, Percent_Identity=33.59375, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788114, Length=409, Percent_Identity=30.5623471882641, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87082077, Length=263, Percent_Identity=27.3764258555133, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786809, Length=214, Percent_Identity=29.9065420560748, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=487, Percent_Identity=40.6570841889117, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6324361, Length=401, Percent_Identity=26.9326683291771, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 56360; Mature: 56360

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNIAKDDFLSLCKDYNTIPLYTEILADSETPFSIFYKLKDEESQNVLLESAETKENWG
CEECCCHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCHHCCC
RYSFICKGKNLRFYQKDDTYFIKDGDTITKGTSKPLEALENLYKSFKFYEDKTLGKFCGG
CEEEEEECCCEEEEECCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGYISYDIVKHYHPIEKYTKAQKSGLDIPDIDMIFTDFLIVFDNLKATIKIIKLIRVRN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKEDYEKALKDIEDIIIKLKNSKGISFLDINNKEPDLKEWSSNMTKEEFINIVNICKNYI
CHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
ENGDVIQVVPSQRFNKKIHTNPINIYRALRYLNPSPYMYFLDFDNVKIIGSSPETLVKIQ
CCCCEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEE
DDTVEIRPIAGTIKRGKTQEEDEELAKKLLSDEKEIAEHVMLVDLARNDIGAIAEPGSVY
CCCEEEEECHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VDNMMHIEKFSHVIHIVSNVYGKKAKQHSIFEVIKHALPAGTLSGAPKVRAMQIIEELEP
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TKRNVYGGAIGYISLNQNIDFAIAIRTATILGNDLYVQAGAGIVADSIPEKEYLESLSKA
HHHCCCCCEEEEEEECCCCCEEEEEEEEEEECCEEEEECCCCCHHCCCCHHHHHHHHHHH
SSVMKAVSVAEASREL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVNIAKDDFLSLCKDYNTIPLYTEILADSETPFSIFYKLKDEESQNVLLESAETKENWG
CEECCCHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCHHCCC
RYSFICKGKNLRFYQKDDTYFIKDGDTITKGTSKPLEALENLYKSFKFYEDKTLGKFCGG
CEEEEEECCCEEEEECCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGYISYDIVKHYHPIEKYTKAQKSGLDIPDIDMIFTDFLIVFDNLKATIKIIKLIRVRN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKEDYEKALKDIEDIIIKLKNSKGISFLDINNKEPDLKEWSSNMTKEEFINIVNICKNYI
CHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
ENGDVIQVVPSQRFNKKIHTNPINIYRALRYLNPSPYMYFLDFDNVKIIGSSPETLVKIQ
CCCCEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEE
DDTVEIRPIAGTIKRGKTQEEDEELAKKLLSDEKEIAEHVMLVDLARNDIGAIAEPGSVY
CCCEEEEECHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VDNMMHIEKFSHVIHIVSNVYGKKAKQHSIFEVIKHALPAGTLSGAPKVRAMQIIEELEP
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TKRNVYGGAIGYISLNQNIDFAIAIRTATILGNDLYVQAGAGIVADSIPEKEYLESLSKA
HHHCCCCCEEEEEEECCCCCEEEEEEEEEEECCEEEEECCCCCHHCCCCHHHHHHHHHHH
SSVMKAVSVAEASREL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]