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The map label for this gene is leuD

Identifier: 195952819

GI number: 195952819

Start: 385701

End: 386198

Strand: Direct

Name: leuD

Synonym: HY04AAS1_0441

Alternate gene names: 195952819

Gene position: 385701-386198 (Clockwise)

Preceding gene: 195952818

Following gene: 195952820

Centisome position: 24.73

GC content: 34.54

Gene sequence:

>498_bases
ATGATTATAAAAGGTAAAATATGGAAGTTTAAAGATAATATAGATACAGATCAGATTATACCTGCAAGATATTTAAACAC
GTCAGACCCTTTGGAACTTGCTTCTCACGTAATGGAAGACTCTGAAAAACCAAACTTTGCGAAAGAGCATCAAGAAGGTG
ATATCATAGTAGCTGGTAAAAATTTTGGTTCTGGCTCTTCTAGGGAACATGCTCCTATAGCTATAAAGTATGCTGGTGTA
CCAGTGGTGGTGGCTAAAAGCTTTGCAAGGATATTTTTTAGAAATGCAATAAACATAGGTCTTCCGATAGTAGAGTGCAA
AGAAGCTGTGGATGAAGCTGAAGATGGAGATATTTTTGAAATAGACCTTGAAAATGGTATAGTAAAAAATGTTACAAAAA
ACAAAACCTATTCTTCTACTAAATTTCCAGAAGAGCTTATGGCTATACTAAGAGCTGGTGGGTTGATGGAATATGCAAAA
TCAAGATTATCTTCTTAA

Upstream 100 bases:

>100_bases
TGGAACTTCAGATAGAGGCTATCAAAAAAGAAAGGCTTAAGTTAAAAGACGAAATGGAGTTAATTGTTGTAGAATATATG
AAAAAGCATGGAGAAGTAGC

Downstream 100 bases:

>100_bases
GTTAAAAGAACTTATAAAAAAATATTCTCTTTTGGTAGCAGATGAACCTAAGTTTAAACTAGTTTCTGGTAAACTTAGCA
GGTACTATATAGATTTAAAA

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIIKGKIWKFKDNIDTDQIIPARYLNTSDPLELASHVMEDSEKPNFAKEHQEGDIIVAGKNFGSGSSREHAPIAIKYAGV
PVVVAKSFARIFFRNAINIGLPIVECKEAVDEAEDGDIFEIDLENGIVKNVTKNKTYSSTKFPEELMAILRAGGLMEYAK
SRLSS

Sequences:

>Translated_165_residues
MIIKGKIWKFKDNIDTDQIIPARYLNTSDPLELASHVMEDSEKPNFAKEHQEGDIIVAGKNFGSGSSREHAPIAIKYAGV
PVVVAKSFARIFFRNAINIGLPIVECKEAVDEAEDGDIFEIDLENGIVKNVTKNKTYSSTKFPEELMAILRAGGLMEYAK
SRLSS
>Mature_165_residues
MIIKGKIWKFKDNIDTDQIIPARYLNTSDPLELASHVMEDSEKPNFAKEHQEGDIIVAGKNFGSGSSREHAPIAIKYAGV
PVVVAKSFARIFFRNAINIGLPIVECKEAVDEAEDGDIFEIDLENGIVKNVTKNKTYSSTKFPEELMAILRAGGLMEYAK
SRLSS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=154, Percent_Identity=35.7142857142857, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320440, Length=95, Percent_Identity=43.1578947368421, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321429, Length=108, Percent_Identity=35.1851851851852, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_HYDS0 (B4U7M0)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002121109.1
- ProteinModelPortal:   B4U7M0
- SMR:   B4U7M0
- GeneID:   6743235
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0441
- HOGENOM:   HBG304838
- OMA:   LASHCMA
- ProtClustDB:   CLSK2803313
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02084
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18275; Mature: 18275

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKGKIWKFKDNIDTDQIIPARYLNTSDPLELASHVMEDSEKPNFAKEHQEGDIIVAGK
CEECCCEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEC
NFGSGSSREHAPIAIKYAGVPVVVAKSFARIFFRNAINIGLPIVECKEAVDEAEDGDIFE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEE
IDLENGIVKNVTKNKTYSSTKFPEELMAILRAGGLMEYAKSRLSS
EECCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MIIKGKIWKFKDNIDTDQIIPARYLNTSDPLELASHVMEDSEKPNFAKEHQEGDIIVAGK
CEECCCEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEC
NFGSGSSREHAPIAIKYAGVPVVVAKSFARIFFRNAINIGLPIVECKEAVDEAEDGDIFE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEE
IDLENGIVKNVTKNKTYSSTKFPEELMAILRAGGLMEYAKSRLSS
EECCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA