| Definition | Hydrogenobaculum sp. Y04AAS1 chromosome, complete genome. |
|---|---|
| Accession | NC_011126 |
| Length | 1,559,514 |
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The map label for this gene is tufA [H]
Identifier: 195952641
GI number: 195952641
Start: 239337
End: 240554
Strand: Direct
Name: tufA [H]
Synonym: HY04AAS1_0263
Alternate gene names: 195952641
Gene position: 239337-240554 (Clockwise)
Preceding gene: 195952640
Following gene: 195952642
Centisome position: 15.35
GC content: 41.22
Gene sequence:
>1218_bases ATGGCAAAGGAAAAGTTTGTTAGAGAGAAAGAGCACATCAACGTAGGTACCATAGGTCACGTTGACCATGGTAAATCTAC CTTAACATCTGCTATCACCTGTGTGTTAGGTGCTGGTGTATTATCAGGCGGTAAGGCAAAATGCTACCGTTATGAAGAAA TAGACAAAGCACCTGAAGAAAAAGAAAGAGGTATTACCATCAACATAACTCACGTTGAATACGAAACACCAAAAAGACAC TACGCTCACGTTGACTGTCCCGGACACGCAGACTACATAAAGAACATGATTACAGGTGCCGCTCAAATGGACGGTGCAAT ACTTGTTGTATCAGCAGCAGATGGTCCAATGCCCCAAACCAGAGAACACGTTTTGCTAGCAAGACAAGTTAACGTCCCTT ACATAGTGGTATTTATGAACAAATGCGATATGGTAGATGATCCAGAGCTATTAGATCTAGTAGAGTTAGAAGTAAGAGAC CTACTAAACAAGTACGAATTTCCGGGTGATGATGTGCCAATTATAAGAGGTTCTGCTTTAGGAGCCCTTGAAGAACTAGA CAAAGGCAAACCAGACAAATGGTGCAATGCAATAGTAGACCTTATGAAAGCCTTAGATGACTATATACCATCTCCTCAAA GAGAAACTGATAAGCCATTCCTAATGCCAATAGAAGATGTCTTTACCATATCTGGTAGAGGTACAGTTGTCACAGGTAGA GTAGAAAGAGGTGTGCTAAAACCAGGCGAAGAAGTGGAAATAGTAGGTCTAAAAGAAGAATCCCTAAAAACTACAGCCAC ATCTGTGGAAATGTTTAGAAAAATCCTTGACGAAGCATTACCCGGTGACAACGTAGGTGTGTTATTAAGAGGTGTAGGTA AAGACCAAGTAGAAAGAGGTCAAGTCTTAGCAAAGCCAGGTTCTATAACTCCACATAAAAAGTTCAAAGCTCAAGTTTAC GTATTGTCAAAAGAAGAAGGTGGTAGACACACTCCATTCTTCCTAAACTACAGACCTCAGTTCTACATAAGAACCGCTGA CGTAACAGGTACAGTGGTAAAGCTTCCAGAAGGTCAAGAGATGGTAATGCCTGGTGATAACGTAGAGTTTGAAGTAGAGC TAATACATCCAGTAGCTATGGAAGAAGGCCTTAGATTTGCCATAAGAGAAGGTGGTAGAACAGTTGGTGCTGGTGTTGTC ACCAAGATTATAGAGTGA
Upstream 100 bases:
>100_bases GGTACATTTATTATGAAATTCTCCCATTACAGTGAAGCACCTCAAAGTATTACAGAGAAAGTAGTTGGAGAAAGAACTCA TACTTAAAAAGGAGTAGCTT
Downstream 100 bases:
>100_bases GGCAGAAAGTATGGAGCAGGATAAAATACGTATAAAGCTTAAATCTTACGATTATAAACTTTTGGATCAATCTGTCAAGC AAATAGTGGAAACAGTTAAA
Product: elongation factor Tu
Products: GDP; phosphate
Alternate protein names: EF-Tu [H]
Number of amino acids: Translated: 405; Mature: 404
Protein sequence:
>405_residues MAKEKFVREKEHINVGTIGHVDHGKSTLTSAITCVLGAGVLSGGKAKCYRYEEIDKAPEEKERGITINITHVEYETPKRH YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDPELLDLVELEVRD LLNKYEFPGDDVPIIRGSALGALEELDKGKPDKWCNAIVDLMKALDDYIPSPQRETDKPFLMPIEDVFTISGRGTVVTGR VERGVLKPGEEVEIVGLKEESLKTTATSVEMFRKILDEALPGDNVGVLLRGVGKDQVERGQVLAKPGSITPHKKFKAQVY VLSKEEGGRHTPFFLNYRPQFYIRTADVTGTVVKLPEGQEMVMPGDNVEFEVELIHPVAMEEGLRFAIREGGRTVGAGVV TKIIE
Sequences:
>Translated_405_residues MAKEKFVREKEHINVGTIGHVDHGKSTLTSAITCVLGAGVLSGGKAKCYRYEEIDKAPEEKERGITINITHVEYETPKRH YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDPELLDLVELEVRD LLNKYEFPGDDVPIIRGSALGALEELDKGKPDKWCNAIVDLMKALDDYIPSPQRETDKPFLMPIEDVFTISGRGTVVTGR VERGVLKPGEEVEIVGLKEESLKTTATSVEMFRKILDEALPGDNVGVLLRGVGKDQVERGQVLAKPGSITPHKKFKAQVY VLSKEEGGRHTPFFLNYRPQFYIRTADVTGTVVKLPEGQEMVMPGDNVEFEVELIHPVAMEEGLRFAIREGGRTVGAGVV TKIIE >Mature_404_residues AKEKFVREKEHINVGTIGHVDHGKSTLTSAITCVLGAGVLSGGKAKCYRYEEIDKAPEEKERGITINITHVEYETPKRHY AHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDPELLDLVELEVRDL LNKYEFPGDDVPIIRGSALGALEELDKGKPDKWCNAIVDLMKALDDYIPSPQRETDKPFLMPIEDVFTISGRGTVVTGRV ERGVLKPGEEVEIVGLKEESLKTTATSVEMFRKILDEALPGDNVGVLLRGVGKDQVERGQVLAKPGSITPHKKFKAQVYV LSKEEGGRHTPFFLNYRPQFYIRTADVTGTVVKLPEGQEMVMPGDNVEFEVELIHPVAMEEGLRFAIREGGRTVGAGVVT KIIE
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG0050
COG function: function code J; GTPases - translation elongation factors
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI34147630, Length=403, Percent_Identity=53.8461538461538, Blast_Score=454, Evalue=1e-127, Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=32.2799097065463, Blast_Score=188, Evalue=9e-48, Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=32.7313769751693, Blast_Score=188, Evalue=1e-47, Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=31.0502283105023, Blast_Score=171, Evalue=9e-43, Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=31.0502283105023, Blast_Score=171, Evalue=2e-42, Organism=Homo sapiens, GI194018522, Length=342, Percent_Identity=29.2397660818713, Blast_Score=130, Evalue=2e-30, Organism=Homo sapiens, GI194018520, Length=342, Percent_Identity=29.2397660818713, Blast_Score=130, Evalue=3e-30, Organism=Homo sapiens, GI194097354, Length=342, Percent_Identity=29.2397660818713, Blast_Score=130, Evalue=3e-30, Organism=Homo sapiens, GI46094014, Length=349, Percent_Identity=27.7936962750716, Blast_Score=127, Evalue=2e-29, Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=27.9503105590062, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI4503507, Length=322, Percent_Identity=28.8819875776398, Blast_Score=82, Evalue=8e-16, Organism=Homo sapiens, GI82546879, Length=436, Percent_Identity=23.394495412844, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI53729339, Length=282, Percent_Identity=31.2056737588652, Blast_Score=74, Evalue=2e-13, Organism=Homo sapiens, GI53729337, Length=282, Percent_Identity=31.2056737588652, Blast_Score=74, Evalue=2e-13, Organism=Homo sapiens, GI45593140, Length=434, Percent_Identity=24.1935483870968, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=33.5820895522388, Blast_Score=67, Evalue=3e-11, Organism=Homo sapiens, GI157426893, Length=244, Percent_Identity=26.6393442622951, Blast_Score=65, Evalue=9e-11, Organism=Escherichia coli, GI1790412, Length=404, Percent_Identity=70.7920792079208, Blast_Score=563, Evalue=1e-161, Organism=Escherichia coli, GI1789737, Length=404, Percent_Identity=70.7920792079208, Blast_Score=562, Evalue=1e-161, Organism=Escherichia coli, GI2367247, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=3e-28, Organism=Escherichia coli, GI48994988, Length=245, Percent_Identity=33.8775510204082, Blast_Score=102, Evalue=3e-23, Organism=Escherichia coli, GI1789108, Length=317, Percent_Identity=28.0757097791798, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1790835, Length=147, Percent_Identity=31.2925170068027, Blast_Score=65, Evalue=7e-12, Organism=Escherichia coli, GI1788922, Length=144, Percent_Identity=32.6388888888889, Blast_Score=63, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17556456, Length=410, Percent_Identity=51.9512195121951, Blast_Score=390, Evalue=1e-109, Organism=Caenorhabditis elegans, GI25141371, Length=396, Percent_Identity=44.9494949494949, Blast_Score=335, Evalue=2e-92, Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=31.8284424379233, Blast_Score=185, Evalue=3e-47, Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=31.8284424379233, Blast_Score=185, Evalue=3e-47, Organism=Caenorhabditis elegans, GI32566303, Length=423, Percent_Identity=31.9148936170213, Blast_Score=164, Evalue=1e-40, Organism=Caenorhabditis elegans, GI115532067, Length=444, Percent_Identity=28.6036036036036, Blast_Score=137, Evalue=7e-33, Organism=Caenorhabditis elegans, GI115532065, Length=444, Percent_Identity=28.6036036036036, Blast_Score=137, Evalue=1e-32, Organism=Caenorhabditis elegans, GI32566629, Length=442, Percent_Identity=27.1493212669683, Blast_Score=135, Evalue=4e-32, Organism=Caenorhabditis elegans, GI17506081, Length=282, Percent_Identity=29.0780141843972, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI32566301, Length=167, Percent_Identity=37.7245508982036, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17557151, Length=241, Percent_Identity=30.7053941908714, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17506493, Length=198, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6324761, Length=404, Percent_Identity=64.8514851485149, Blast_Score=526, Evalue=1e-150, Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.7567567567568, Blast_Score=177, Evalue=2e-45, Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.7567567567568, Blast_Score=177, Evalue=2e-45, Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=25.9340659340659, Blast_Score=104, Evalue=3e-23, Organism=Saccharomyces cerevisiae, GI6320377, Length=350, Percent_Identity=27.7142857142857, Blast_Score=98, Evalue=3e-21, Organism=Saccharomyces cerevisiae, GI6320863, Length=283, Percent_Identity=28.2685512367491, Blast_Score=79, Evalue=2e-15, Organism=Saccharomyces cerevisiae, GI6323320, Length=258, Percent_Identity=28.6821705426357, Blast_Score=79, Evalue=2e-15, Organism=Saccharomyces cerevisiae, GI6324707, Length=182, Percent_Identity=32.967032967033, Blast_Score=63, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6320593, Length=182, Percent_Identity=32.967032967033, Blast_Score=63, Evalue=7e-11, Organism=Drosophila melanogaster, GI281363316, Length=402, Percent_Identity=55.7213930348259, Blast_Score=444, Evalue=1e-125, Organism=Drosophila melanogaster, GI17864358, Length=402, Percent_Identity=55.7213930348259, Blast_Score=444, Evalue=1e-125, Organism=Drosophila melanogaster, GI19921738, Length=396, Percent_Identity=47.979797979798, Blast_Score=372, Evalue=1e-103, Organism=Drosophila melanogaster, GI45553807, Length=458, Percent_Identity=32.5327510917031, Blast_Score=183, Evalue=2e-46, Organism=Drosophila melanogaster, GI45553816, Length=458, Percent_Identity=32.5327510917031, Blast_Score=183, Evalue=2e-46, Organism=Drosophila melanogaster, GI24651721, Length=458, Percent_Identity=32.5327510917031, Blast_Score=183, Evalue=2e-46, Organism=Drosophila melanogaster, GI17864154, Length=458, Percent_Identity=32.5327510917031, Blast_Score=183, Evalue=2e-46, Organism=Drosophila melanogaster, GI24652838, Length=452, Percent_Identity=31.858407079646, Blast_Score=178, Evalue=6e-45, Organism=Drosophila melanogaster, GI17137572, Length=452, Percent_Identity=31.858407079646, Blast_Score=178, Evalue=6e-45, Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=31.700288184438, Blast_Score=137, Evalue=2e-32, Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=29.1284403669725, Blast_Score=133, Evalue=2e-31, Organism=Drosophila melanogaster, GI19922690, Length=312, Percent_Identity=29.4871794871795, Blast_Score=124, Evalue=9e-29, Organism=Drosophila melanogaster, GI24647048, Length=320, Percent_Identity=27.8125, Blast_Score=87, Evalue=2e-17, Organism=Drosophila melanogaster, GI24647046, Length=320, Percent_Identity=27.8125, Blast_Score=87, Evalue=2e-17, Organism=Drosophila melanogaster, GI78706572, Length=274, Percent_Identity=26.2773722627737, Blast_Score=73, Evalue=4e-13, Organism=Drosophila melanogaster, GI24585711, Length=197, Percent_Identity=31.4720812182741, Blast_Score=66, Evalue=4e-11, Organism=Drosophila melanogaster, GI24585713, Length=197, Percent_Identity=31.4720812182741, Blast_Score=66, Evalue=4e-11, Organism=Drosophila melanogaster, GI24585709, Length=197, Percent_Identity=31.4720812182741, Blast_Score=66, Evalue=4e-11, Organism=Drosophila melanogaster, GI28572034, Length=169, Percent_Identity=35.5029585798817, Blast_Score=66, Evalue=5e-11,
Paralogues:
None
Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004161 - InterPro: IPR004541 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: 3.6.5.3
Molecular weight: Translated: 44729; Mature: 44598
Theoretical pI: Translated: 5.26; Mature: 5.26
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKEKFVREKEHINVGTIGHVDHGKSTLTSAITCVLGAGVLSGGKAKCYRYEEIDKAPEE CCCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCH KERGITINITHVEYETPKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT HHCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC REHVLLARQVNVPYIVVFMNKCDMVDDPELLDLVELEVRDLLNKYEFPGDDVPIIRGSAL HHHEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHH GALEELDKGKPDKWCNAIVDLMKALDDYIPSPQRETDKPFLMPIEDVFTISGRGTVVTGR HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEECCCCEEEECC VERGVLKPGEEVEIVGLKEESLKTTATSVEMFRKILDEALPGDNVGVLLRGVGKDQVERG HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCC QVLAKPGSITPHKKFKAQVYVLSKEEGGRHTPFFLNYRPQFYIRTADVTGTVVKLPEGQE CEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCEEEEEEECCCEEEEECCCCCE MVMPGDNVEFEVELIHPVAMEEGLRFAIREGGRTVGAGVVTKIIE EEECCCCCEEEEEEECCHHHHCCCEEEECCCCCEECHHHHHHHCC >Mature Secondary Structure AKEKFVREKEHINVGTIGHVDHGKSTLTSAITCVLGAGVLSGGKAKCYRYEEIDKAPEE CCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCH KERGITINITHVEYETPKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT HHCCEEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC REHVLLARQVNVPYIVVFMNKCDMVDDPELLDLVELEVRDLLNKYEFPGDDVPIIRGSAL HHHEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHH GALEELDKGKPDKWCNAIVDLMKALDDYIPSPQRETDKPFLMPIEDVFTISGRGTVVTGR HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEECCCCEEEECC VERGVLKPGEEVEIVGLKEESLKTTATSVEMFRKILDEALPGDNVGVLLRGVGKDQVERG HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCC QVLAKPGSITPHKKFKAQVYVLSKEEGGRHTPFFLNYRPQFYIRTADVTGTVVKLPEGQE CEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCEEEEEEECCCEEEEECCCCCE MVMPGDNVEFEVELIHPVAMEEGLRFAIREGGRTVGAGVVTKIIE EEECCCCCEEEEEEECCHHHHCCCEEEECCCCCEECHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: GTP; H2O
Specific reaction: GTP + H2O = GDP + phosphate
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]