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The map label for this gene is hyfR [H]

Identifier: 195952483

GI number: 195952483

Start: 96036

End: 97595

Strand: Direct

Name: hyfR [H]

Synonym: HY04AAS1_0103

Alternate gene names: 195952483

Gene position: 96036-97595 (Clockwise)

Preceding gene: 195952482

Following gene: 195952486

Centisome position: 6.16

GC content: 35.71

Gene sequence:

>1560_bases
ATGGATTTTAGTATACTCGATGAGCTTCTTGACGGGGTTTTGGTAATAGGCTTAGACTATAAGATAGTTTATGCCAACGA
TTCTGCTAAAAAGCTTCTTGACGTAAAAGAAAACGATTTTTGTAAAGGACTTTTTAGTATATGTAATTCTTGTCCCTTAA
AGCTTGTTTTGGAAGAGAGAGAAAGCGTGCAGGTTTACGATATAAAAACCAACACAGATGTTCATGCTTGTTGGAGTATG
AGCCCTCTTTTTAATGGTGATAAATGCGAGGGGGTAGTGGAGGTCTTTAAAGATGTAAGCAACGTAGTTTCTTGTATATT
AGAATCTCAAAGACAAAAAACCTACAAAGAAACAATTTTAAACTCTATAGTAGAGGCAATACTTGTTTTGGATGAAAACG
GTTATATAGTAGAGCACAACAATATAGCCAAAAAAATGCTTTGTAGAACCAGCGAAGAATCTTTGATAGGAAGGCATATA
AAAGAGCTTGTGAATCTGAGTTTAGAAGAGTTTCCTCCACAAGGAGAAAGAACAGATGTATACATAGAAACTCCGTGTGG
TAGGCAAAAAGCATCAACGCTTGTATCCCCTATGAATCTTGGTTTTGGTTATGTGGTATCTTTTTATGTGATCCAGCAAG
ATAATATGTCTTTTTGCGATAAACTAGATTACATTTTTACCAAAAGCCCAACATTTCAAAAAGTACTTGATTTAGTGAAA
TCTTGTTCAGAGCACAATGTAAACGTGCTTATAGAAGGAGAAACCGGCATAGGCAAAAGCCTTTTGGCAAAATACATACA
TCTTTTGTCCCCAAGAAGAGATGCTCCTTTTGTTAAAGTAAACTGTGCTGCTATACCAGAGAACCTCTTGGAGGCAGAGC
TTTTTGGATACGTAAAAGGAGCTTTTACAGGGGCCACAAAAGATAAACCCGGTAAAGCGGAGATAGCTGATGGCGGAACT
TTGTTTTTGGATGAGATAGGGGATATGCCACTGAGCTTACAGGCAAAAATTCTCCACCTTGTGGAAGAAAAAGAATTTGA
AAGGCTTGGCTCAAACACCCCTAGAAAGACAAATACAAGGATTATAGCTTCCACCAACAAAGACATAAAAGAGCTTATGA
GACAAGGACGTTTTAGAGAAGATCTTTATTACAGGTTAAGTGTTGTGAACATATATCTACCCCCTCTTAGAGAAAGAAAA
GAAGATATACCGCTTTTAATAAATAATTTTATAGAAAAATATTCAAAACTCTATTCAAAAAGGGTAAAATCAATATCTGG
TGATGCTATAAAAATGCTCCTTGGTTATGATTTTCCAGGTAACATCAGAGAATTAGAGCATATTATAGAAAAGGCTGTAA
TAACATGTAGAGGTAGCATAATAAATATCGAAGATATAAGTTTAGATTTTCAAAGCAACAACCATAAACAGCAAGAAGAA
AAAGAAAGGATAAAGGCTGTCTTAGAAAAGGTAAATTACAACAAGAGTTTGGCGGCAAAAATGCTTGGTATACACCGTAC
CACTCTTTGGAGAAAGTTAAAAGAGCTTGGTATTGGCTAA

Upstream 100 bases:

>100_bases
AGGGTTCTATGATAACAGCTATGAAATATATGGGAGCTTTTGAGGAGAGCTTGAGGATGATGGGTAGCGTACCTGTGGAT
TGGAATGCTTGATATGTTTT

Downstream 100 bases:

>100_bases
TAGAGTGCTATATTATAAAAATTTATAAAAACCCCTTTACAAAATTAAAAGTTGTGCTATAATGTATATACTACGGGGTG
TGGCTCAGGTGGTAGAGCGT

Product: PAS modulated sigma54 specific transcriptional regulator, Fis family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MDFSILDELLDGVLVIGLDYKIVYANDSAKKLLDVKENDFCKGLFSICNSCPLKLVLEERESVQVYDIKTNTDVHACWSM
SPLFNGDKCEGVVEVFKDVSNVVSCILESQRQKTYKETILNSIVEAILVLDENGYIVEHNNIAKKMLCRTSEESLIGRHI
KELVNLSLEEFPPQGERTDVYIETPCGRQKASTLVSPMNLGFGYVVSFYVIQQDNMSFCDKLDYIFTKSPTFQKVLDLVK
SCSEHNVNVLIEGETGIGKSLLAKYIHLLSPRRDAPFVKVNCAAIPENLLEAELFGYVKGAFTGATKDKPGKAEIADGGT
LFLDEIGDMPLSLQAKILHLVEEKEFERLGSNTPRKTNTRIIASTNKDIKELMRQGRFREDLYYRLSVVNIYLPPLRERK
EDIPLLINNFIEKYSKLYSKRVKSISGDAIKMLLGYDFPGNIRELEHIIEKAVITCRGSIINIEDISLDFQSNNHKQQEE
KERIKAVLEKVNYNKSLAAKMLGIHRTTLWRKLKELGIG

Sequences:

>Translated_519_residues
MDFSILDELLDGVLVIGLDYKIVYANDSAKKLLDVKENDFCKGLFSICNSCPLKLVLEERESVQVYDIKTNTDVHACWSM
SPLFNGDKCEGVVEVFKDVSNVVSCILESQRQKTYKETILNSIVEAILVLDENGYIVEHNNIAKKMLCRTSEESLIGRHI
KELVNLSLEEFPPQGERTDVYIETPCGRQKASTLVSPMNLGFGYVVSFYVIQQDNMSFCDKLDYIFTKSPTFQKVLDLVK
SCSEHNVNVLIEGETGIGKSLLAKYIHLLSPRRDAPFVKVNCAAIPENLLEAELFGYVKGAFTGATKDKPGKAEIADGGT
LFLDEIGDMPLSLQAKILHLVEEKEFERLGSNTPRKTNTRIIASTNKDIKELMRQGRFREDLYYRLSVVNIYLPPLRERK
EDIPLLINNFIEKYSKLYSKRVKSISGDAIKMLLGYDFPGNIRELEHIIEKAVITCRGSIINIEDISLDFQSNNHKQQEE
KERIKAVLEKVNYNKSLAAKMLGIHRTTLWRKLKELGIG
>Mature_519_residues
MDFSILDELLDGVLVIGLDYKIVYANDSAKKLLDVKENDFCKGLFSICNSCPLKLVLEERESVQVYDIKTNTDVHACWSM
SPLFNGDKCEGVVEVFKDVSNVVSCILESQRQKTYKETILNSIVEAILVLDENGYIVEHNNIAKKMLCRTSEESLIGRHI
KELVNLSLEEFPPQGERTDVYIETPCGRQKASTLVSPMNLGFGYVVSFYVIQQDNMSFCDKLDYIFTKSPTFQKVLDLVK
SCSEHNVNVLIEGETGIGKSLLAKYIHLLSPRRDAPFVKVNCAAIPENLLEAELFGYVKGAFTGATKDKPGKAEIADGGT
LFLDEIGDMPLSLQAKILHLVEEKEFERLGSNTPRKTNTRIIASTNKDIKELMRQGRFREDLYYRLSVVNIYLPPLRERK
EDIPLLINNFIEKYSKLYSKRVKSISGDAIKMLLGYDFPGNIRELEHIIEKAVITCRGSIINIEDISLDFQSNNHKQQEE
KERIKAVLEKVNYNKSLAAKMLGIHRTTLWRKLKELGIG

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=43.0678466076696, Blast_Score=278, Evalue=8e-76,
Organism=Escherichia coli, GI1788550, Length=321, Percent_Identity=43.9252336448598, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1790437, Length=296, Percent_Identity=45.2702702702703, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1789087, Length=306, Percent_Identity=44.4444444444444, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788905, Length=299, Percent_Identity=40.133779264214, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1790299, Length=351, Percent_Identity=37.3219373219373, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=430, Percent_Identity=33.2558139534884, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=42.3076923076923, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI87082152, Length=312, Percent_Identity=39.1025641025641, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=37.5816993464052, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=33.4365325077399, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081858, Length=458, Percent_Identity=27.0742358078603, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789828, Length=303, Percent_Identity=31.6831683168317, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58843; Mature: 58843

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSILDELLDGVLVIGLDYKIVYANDSAKKLLDVKENDFCKGLFSICNSCPLKLVLEER
CCHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
ESVQVYDIKTNTDVHACWSMSPLFNGDKCEGVVEVFKDVSNVVSCILESQRQKTYKETIL
CCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSIVEAILVLDENGYIVEHNNIAKKMLCRTSEESLIGRHIKELVNLSLEEFPPQGERTDV
HHHHHHHEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEEE
YIETPCGRQKASTLVSPMNLGFGYVVSFYVIQQDNMSFCDKLDYIFTKSPTFQKVLDLVK
EEECCCCCHHHHHHHCHHHCCHHHEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHH
SCSEHNVNVLIEGETGIGKSLLAKYIHLLSPRRDAPFVKVNCAAIPENLLEAELFGYVKG
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHH
AFTGATKDKPGKAEIADGGTLFLDEIGDMPLSLQAKILHLVEEKEFERLGSNTPRKTNTR
HCCCCCCCCCCCCEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
IIASTNKDIKELMRQGRFREDLYYRLSVVNIYLPPLRERKEDIPLLINNFIEKYSKLYSK
EEEECCHHHHHHHHCCCCHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RVKSISGDAIKMLLGYDFPGNIRELEHIIEKAVITCRGSIINIEDISLDFQSNNHKQQEE
HHHHCCCCCEEHEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
KERIKAVLEKVNYNKSLAAKMLGIHRTTLWRKLKELGIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFSILDELLDGVLVIGLDYKIVYANDSAKKLLDVKENDFCKGLFSICNSCPLKLVLEER
CCHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
ESVQVYDIKTNTDVHACWSMSPLFNGDKCEGVVEVFKDVSNVVSCILESQRQKTYKETIL
CCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSIVEAILVLDENGYIVEHNNIAKKMLCRTSEESLIGRHIKELVNLSLEEFPPQGERTDV
HHHHHHHEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEEE
YIETPCGRQKASTLVSPMNLGFGYVVSFYVIQQDNMSFCDKLDYIFTKSPTFQKVLDLVK
EEECCCCCHHHHHHHCHHHCCHHHEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHH
SCSEHNVNVLIEGETGIGKSLLAKYIHLLSPRRDAPFVKVNCAAIPENLLEAELFGYVKG
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHH
AFTGATKDKPGKAEIADGGTLFLDEIGDMPLSLQAKILHLVEEKEFERLGSNTPRKTNTR
HCCCCCCCCCCCCEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
IIASTNKDIKELMRQGRFREDLYYRLSVVNIYLPPLRERKEDIPLLINNFIEKYSKLYSK
EEEECCHHHHHHHHCCCCHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
RVKSISGDAIKMLLGYDFPGNIRELEHIIEKAVITCRGSIINIEDISLDFQSNNHKQQEE
HHHHCCCCCEEHEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
KERIKAVLEKVNYNKSLAAKMLGIHRTTLWRKLKELGIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]