Click here to switch to the map view.

The map label for this gene is mdtK [H]

Identifier: 194738332

GI number: 194738332

Start: 1465876

End: 1467249

Strand: Reverse

Name: mdtK [H]

Synonym: SeSA_A1521

Alternate gene names: 194738332

Gene position: 1467249-1465876 (Counterclockwise)

Preceding gene: 194736258

Following gene: 194738250

Centisome position: 31.16

GC content: 53.86

Gene sequence:

>1374_bases
GTGCAGAAGTATACCAGTGAAGCGCGTCAGCTATTAGCGTTAGCGATACCGGTGATCCTTGCGCAAGTGGCACAGACCGC
AATGGGATTCGTCGATACCGTCATGGCAGGAGGCTATAGCGCTACGGACATGGCTGCCGTCGCTATCGGCACCTCTATCT
GGCTTCCCGCCATTTTGTTTGGTCACGGCTTACTGCTGGCCTTGACGCCTGTGATTGCGCAACTCAATGGCTCCGGCCGT
CGTGAGCGTATCGCGCATCAGGTTCGACAAGGGTTCTGGCTGGCGGGCTTTGTCTCCGTGCTGGTGATGATCGTCCTGTG
GAATGCCGGATACATTATCCGCTCTATGCATAATATTGATCCGGCGCTGGCCGATAAAGCCGTGGGTTATCTTCGCGCGC
TACTGTGGGGCGCGCCAGGATACCTTTTCTTTCAGGTAGCGCGAAACCAGTGCGAAGGTCTTGCCAAAACCAAACCGGGT
ATGGTGATGGGATTTTTGGGCCTGCTGGTGAATATTCCGGTGAACTATATTTTTATCTATGGCCATTTTGGTATGCCTGA
ACTCGGCGGTATCGGCTGCGGCGTTGCGACCGCGGCGGTCTATTGGGTGATGTTTATCGCCATGCTTTCTTACATTAAAC
ACGCGCGTTCAATGCGTGATATTCGTAATGAAACAGGCTTTGGCAAACCCGATAGCGTCGTCATGAAGCGCCTGATTCAA
CTAGGGTTACCGATTGCGCTGGCGCTGTTCTTTGAAGTGACGTTGTTTGCGGTCGTTGCGCTGCTGGTTTCGCCATTAGG
TATTGTGGATGTCGCGGGTCATCAGATTGCGCTTAACTTTAGCTCGCTGATGTTCGTACTGCCGATGTCGCTGGCTGCGG
CGGTAACGATTCGAGTGGGTTATCGTCTGGGCCAGGGCTCAACGCTGGATGCGCAAACCGCAGCGCGTACCGGTTTAGGC
GTCGGCATTTGTATGGCTGTGGTTACCGCGATCTTTACCGTTACTCTGCGTAAGCATATTGCTTTGCTCTACAATGACAA
CCCCGAGGTCGTGGCGCTTGCCGCGCAATTAATGCTGCTCGCCGCGGTTTATCAGATTTCCGACTCTATACAGGTTATCG
GCAGCGGTATTCTGCGCGGTTATAAAGATACGCGTTCCATCTTTTTTATCACCTTTACCGCCTACTGGGTGTTAGGTTTA
CCCAGCGGTTATATCCTGGCGTTAACGGATCTGGTGGTGGATCGTATGGGACCGGCAGGCTTCTGGATGGGGTTCATCAT
CGGCCTGACCTCGGCGGCGGTGTTAATGATGCTACGGATGCGCTATCTACAACGTCAGCCGTCCGCCATCATTTTGCAGC
GCGCTGCGCGATAA

Upstream 100 bases:

>100_bases
CCCCGCTACAATAGACTGAAATTTCCCCTGCTATTTCTTTTGGCTGCCACTACGGCGGCTTTTTAGTCTTTCATATAACA
ACAAAATAAAAGGTGAATAC

Downstream 100 bases:

>100_bases
TACGACGATAGCCGTCGCATGGCGGCTATTTTCTCGACATGTTGCATATTTCCACCGCAATCAGGTGATTTCAGGAAGAA
AATTGCGATTTCCCTCTTGC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNETGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR

Sequences:

>Translated_457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNETGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
>Mature_457_residues
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFLGLLVNIPVNYIFIYGHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNETGFGKPDSVVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLG
VGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGL
PSGYILALTDLVVDRMGPAGFWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=426, Percent_Identity=21.3615023474178, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI22907060, Length=451, Percent_Identity=21.7294900221729, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI38261960, Length=462, Percent_Identity=20.995670995671, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=92.5601750547046, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=458, Percent_Identity=24.235807860262, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320545, Length=439, Percent_Identity=20.9567198177677, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=390, Percent_Identity=20, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49400; Mature: 49400

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFLGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNETGFGKPDSVVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLGVGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLL
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVDRMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MQKYTSEARQLLALAIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFLGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNETGFGKPDSVVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLGVGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLL
EEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVDRMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWMGFIIGLTSAAVLMMLRMRYLQRQPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA