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The map label for this gene is artJ [H]

Identifier: 194738205

GI number: 194738205

Start: 4494919

End: 4495659

Strand: Reverse

Name: artJ [H]

Synonym: SeSA_A4620

Alternate gene names: 194738205

Gene position: 4495659-4494919 (Counterclockwise)

Preceding gene: 194736736

Following gene: 194735865

Centisome position: 95.47

GC content: 48.72

Gene sequence:

>741_bases
ATGAAAAAAAAACTTATTGTCATGCTGTTAGCCAGCCTCTCCGTTCACGCCGCTTCCGTTTCCGCCAGAACATTACATTT
TGGCACCTCCGCCACCTATGCGCCCTATGAGTTTGTCGATGCCGATAATAAAATTGTTGGTTTCGATATTGATGTCGCCA
ACGCGGTCTGCAAAGAGATGCAAGCGGAGTGCTCATTCACTAACCAGAGCTTTGATAGTCTGATCCCCGGCCTACGCTTC
AAAAAGTTTGATGCGGTGATCGCCGGTATGGATATGACGCCCAAACGTGAGCAGCAGGTCTCCTTTAGCCAACCCTATTA
CGAAGGTTTATCGGCGGTAGTCGTCACGCGTAAAGGGGCTTATCACACTTTTGCCGATCTGAAAGGCAAAAAAGTCGGTC
TGGAAAACGGTACAACGCATCAGCGCTATTTGCAGGATAAACAACAAGCTATCACGCCTGTCGCCTATGACAGCTATCTA
AACGCCTTTACCGATCTGAAAAATAACCGTCTTGAGGGCGTGTTTGGCGATGTCGCCGCGATTGGAAAGTGGCTAAAAAA
CAATCCGGATTACGCCATCATGGATGAACGCGCCAGCGATCCTGACTACTACGGAAAAGGGTTAGGCATTGCGGTACGCA
AAGGCAATGACGCGCTATTGCAAGAAATTAATGCCGCGCTCGACAAGGTGAAAGCGTCGCCGGAATATGCGCAAATGCAA
GAGAAATGGTTTACGCAGTAA

Upstream 100 bases:

>100_bases
TTTTCACGCCACGCCTAAAGGCACAGAATCAGAATAGCACCCTGCGCCAAAAAAAGAATAGCACGGTGACCACAACATCC
AATTGATATCAGGGATCAAG

Downstream 100 bases:

>100_bases
TTCAGTGGCGGGAGAGCTGCCGGATGAGACGCTCTGGCGTTGCCATCCGGCATGTTACGCCTCACAGCTTAATGAAATGC
TCGCGGTAGTAAGCCAGCTC

Product: arginine-binding periplasmic protein 2

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKKLIVMLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRF
KKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYL
NAFTDLKNNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQ
EKWFTQ

Sequences:

>Translated_246_residues
MKKKLIVMLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRF
KKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYL
NAFTDLKNNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQ
EKWFTQ
>Mature_246_residues
MKKKLIVMLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRF
KKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYL
NAFTDLKNNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQ
EKWFTQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=56.5040650406504, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1787088, Length=244, Percent_Identity=53.2786885245902, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=35.5731225296443, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=32.8947368421053, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788228, Length=251, Percent_Identity=30.6772908366534, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=29.4354838709677, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27320; Mature: 27320

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLIVMLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEM
CCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHH
QAECSFTNQSFDSLIPGLRFKKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGA
HHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCEEEEEECCCC
YHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLKNNRLEGVFGDVAA
CHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
IGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQ
HHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHH
EKWFTQ
HHHCCC
>Mature Secondary Structure
MKKKLIVMLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEM
CCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHH
QAECSFTNQSFDSLIPGLRFKKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGA
HHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCEEEEEECCCC
YHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLKNNRLEGVFGDVAA
CHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
IGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQ
HHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHH
EKWFTQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]