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The map label for this gene is cobJ [H]

Identifier: 194738094

GI number: 194738094

Start: 2085528

End: 2086253

Strand: Reverse

Name: cobJ [H]

Synonym: SeSA_A2196

Alternate gene names: 194738094

Gene position: 2086253-2085528 (Counterclockwise)

Preceding gene: 194737338

Following gene: 194736499

Centisome position: 44.3

GC content: 56.61

Gene sequence:

>726_bases
ATGTTGAGCGTAATTGGTATTGGCCCCGGTTCGCAGGCAATGATGACGATGGAGGCGATTGAGGCGCTTCAGGCGGCGGA
AATCGTCGTGGGCTACAAAACCTATACCCATCTGGTTAAAGCATTTACCGGCGATAAACAGGTGATCAAGACCGGCATGT
GTAAGGAAATTGAACGCTGCCAGGCGGCTATTGAACTGGCGCAGGCCGGGCATAACGTCGCGCTCATCAGCAGCGGGGAT
GCGGGAATTTACGGCATGGCGGGTCTGGTGCTGGAACTGGTCGGCAAGCAAAAACTGGACGTCGAGGTGCGGCTCATTCC
TGGCATGACCGCGAGCATCGCCGCCGCCTCTTTACTGGGCGCGCCGCTGATGCATGACTTCTGCCATATCAGCCTCAGCG
ACCTGCTGACCCCGTGGCCGGTAATTGAAAAGCGCATCGTTGCCGCCGGAGAGGCAGACTTTGTTATCTGTTTTTACAAC
CCGCGCAGCCGCGGGCGCGAAGGGCATCTGGCGCGCGCGTTTGACCTGCTTGCCGCCAGTAAAAGCGCGCAAACGCCGGT
TGGCGTGGTGAAATCCGCCGGACGTAAGAAAGAGGAAAAATGGCTGACTACGCTCGGCGATATGGATTTTGAACCGGTAG
ATATGACCAGTCTGGTCATCGTTGGTAATAAAACGACCTATGTTCAGGATGGGCTGATGATAACGCCGCGAGGTTACACG
CTGTGA

Upstream 100 bases:

>100_bases
GCGTATCCGGCCCGGCTGCGTGGTTATTAAGCCAGGGACAATTGTTAGGCGAGACCCTGCGAGAACAGGGCGTCACTATT
ACTTTAGGAGTTGCACACTG

Downstream 100 bases:

>100_bases
ATGAGGGAGATGTGCTGGTGGTGGGCGGCACCAGCGATGCGCGCGCGTTATGCCGACAACTGGATGCGGCGAACGTCGCA
TACACCTTATCCGTGGCGAC

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCKEIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVGKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L

Sequences:

>Translated_241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCKEIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVGKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L
>Mature_241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCKEIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVGKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 25812; Mature: 25812

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCKEIERC
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVGKQKLDVEVRLIPGMTASIAAASLLG
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHC
APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS
CHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEECCCCCC
L
C
>Mature Secondary Structure
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCKEIERC
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVGKQKLDVEVRLIPGMTASIAAASLLG
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHC
APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS
CHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEECCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]