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The map label for this gene is yneJ [H]

Identifier: 194738004

GI number: 194738004

Start: 1567669

End: 1568541

Strand: Reverse

Name: yneJ [H]

Synonym: SeSA_A1628

Alternate gene names: 194738004

Gene position: 1568541-1567669 (Counterclockwise)

Preceding gene: 194735448

Following gene: 194734140

Centisome position: 33.31

GC content: 57.16

Gene sequence:

>873_bases
ATGGACCTGACGCAACTGGAAATGTTTAACGCTGTCGCTCTGACGGGCAGCATTACGCAGGCGGCGGCAAAAGTGCACCG
CGTTCCGTCCAACCTGACCACGCGTATCCGCCAGCTTGAAGCCGACCTGGGCGTCTCTCTGTTTATTCGCGAAAACCAGC
GACTCAGGCTGTCACCAGCCGGGCATAACTTTTTACGTTACAGCCAGCAGATCCTGGCGCTGGTCGATGAAGCCCGCATG
GTGGTTGCCGGAGAGGAACCGCAAGGACTGTTTTCACTCGGCGCGTTAGAAAGTACTGCCGCCGTACGTATCCCCGCGCT
GCTGGCGGGTTATAACCAGCGTTACCCTAAAATTCAGTTCGCGTTAACGACCGGTCCTTCCGGCGCCATGCTGGATGGCG
TGCTCGAGGGAAAGCTGAACGCCGCCTTTATTGATGGCCCGCTAATGCATCCGGGGCTGGAAGGTATACCGGCTTACCAG
GAAGAGATGATGATCGTCGCGCCACACGGCCATTCCGTCGTTTCGCGGGCCAGCGAGGTGAATGGTTATAACATCTATGC
TTTTCGCGCCAACTGTTCTTACCGACGGCATTTCGAAAGCTGGTTTCATGCCGATGGCGCGACCCCCGGTACGATTCACG
AAATGGAGTCATATCATGGAATGTTGGCGTGCGTAATTGCCGGCGCAGGTATTGCGCTGATGCCAGCAAGTATGCTCAAC
AGTATGCCAGGACATCATCAGGTGGAAGCCTGGCCGTTGGCGGAGAAGTGGCGCTGGCTTAACACCTGGCTAATGTGGCG
GCGCGGGGCGATGACGCGTCAGCTTGAGGCCTTTATCGAGTTACTTAACGCCCAACTGGCGAGCGTCGACTGA

Upstream 100 bases:

>100_bases
TCTTTCTCTCGTTATCAGGGGAGTTGTACCATCATGACGCCCTTTACCCTTTCTGAAAAATGAATAATATTAACCAGACT
ATTCACGAATTGAGAAAACC

Downstream 100 bases:

>100_bases
AGACGAAACGGCCTGCGAAGTAAATGAGAAGCAGGTCACAAAATGATTGTCTTTTCGCCAGTAAACGTTTTAAGATCGCG
GTCTCACCTTCTCGCAACTA

Product: putative LysR substrate binding domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDLTQLEMFNAVALTGSITQAAAKVHRVPSNLTTRIRQLEADLGVSLFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGEEPQGLFSLGALESTAAVRIPALLAGYNQRYPKIQFALTTGPSGAMLDGVLEGKLNAAFIDGPLMHPGLEGIPAYQ
EEMMIVAPHGHSVVSRASEVNGYNIYAFRANCSYRRHFESWFHADGATPGTIHEMESYHGMLACVIAGAGIALMPASMLN
SMPGHHQVEAWPLAEKWRWLNTWLMWRRGAMTRQLEAFIELLNAQLASVD

Sequences:

>Translated_290_residues
MDLTQLEMFNAVALTGSITQAAAKVHRVPSNLTTRIRQLEADLGVSLFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGEEPQGLFSLGALESTAAVRIPALLAGYNQRYPKIQFALTTGPSGAMLDGVLEGKLNAAFIDGPLMHPGLEGIPAYQ
EEMMIVAPHGHSVVSRASEVNGYNIYAFRANCSYRRHFESWFHADGATPGTIHEMESYHGMLACVIAGAGIALMPASMLN
SMPGHHQVEAWPLAEKWRWLNTWLMWRRGAMTRQLEAFIELLNAQLASVD
>Mature_290_residues
MDLTQLEMFNAVALTGSITQAAAKVHRVPSNLTTRIRQLEADLGVSLFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGEEPQGLFSLGALESTAAVRIPALLAGYNQRYPKIQFALTTGPSGAMLDGVLEGKLNAAFIDGPLMHPGLEGIPAYQ
EEMMIVAPHGHSVVSRASEVNGYNIYAFRANCSYRRHFESWFHADGATPGTIHEMESYHGMLACVIAGAGIALMPASMLN
SMPGHHQVEAWPLAEKWRWLNTWLMWRRGAMTRQLEAFIELLNAQLASVD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=84.452296819788, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=25.1677852348993, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI157672245, Length=266, Percent_Identity=25.187969924812, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=25.4980079681275, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=25.296442687747, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082132, Length=244, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=25.1748251748252, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=167, Percent_Identity=28.1437125748503, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=285, Percent_Identity=21.4035087719298, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693105, Length=260, Percent_Identity=20.3846153846154, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786401, Length=165, Percent_Identity=26.0606060606061, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=303, Percent_Identity=22.1122112211221, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31982; Mature: 31982

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVALTGSITQAAAKVHRVPSNLTTRIRQLEADLGVSLFIRENQRLRLSPA
CCCHHHHHHHHHEECCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARMVVAGEEPQGLFSLGALESTAAVRIPALLAGYNQRYPKIQF
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEE
ALTTGPSGAMLDGVLEGKLNAAFIDGPLMHPGLEGIPAYQEEMMIVAPHGHSVVSRASEV
EEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHC
NGYNIYAFRANCSYRRHFESWFHADGATPGTIHEMESYHGMLACVIAGAGIALMPASMLN
CCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEKWRWLNTWLMWRRGAMTRQLEAFIELLNAQLASVD
CCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLTQLEMFNAVALTGSITQAAAKVHRVPSNLTTRIRQLEADLGVSLFIRENQRLRLSPA
CCCHHHHHHHHHEECCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARMVVAGEEPQGLFSLGALESTAAVRIPALLAGYNQRYPKIQF
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCEEEE
ALTTGPSGAMLDGVLEGKLNAAFIDGPLMHPGLEGIPAYQEEMMIVAPHGHSVVSRASEV
EEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHC
NGYNIYAFRANCSYRRHFESWFHADGATPGTIHEMESYHGMLACVIAGAGIALMPASMLN
CCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEKWRWLNTWLMWRRGAMTRQLEAFIELLNAQLASVD
CCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]