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The map label for this gene is vsr [H]

Identifier: 194737933

GI number: 194737933

Start: 2051045

End: 2051515

Strand: Reverse

Name: vsr [H]

Synonym: SeSA_A2150

Alternate gene names: 194737933

Gene position: 2051515-2051045 (Counterclockwise)

Preceding gene: 194734985

Following gene: 194737004

Centisome position: 43.57

GC content: 56.69

Gene sequence:

>471_bases
ATGGCGGACGTTCACGATAAAGCCACGCGCAGTAAAAATATGCGCGCGATAGCGACCCGCGATACGGCTATTGAAAAACG
TCTTGCCGGCCTGCTAAGCGCGCAGGGGATTACGTTTCATACTCAGGACGCGACATTACCGGGCAAGCCGGACTTTGTGG
TGAATGACTATGATTGTGTGATTTTTACTCACGGCTGCTTTTGGCATCATCACCACTGTTATCTGTTCAAAGTACCGGCC
ACGCGAACGGCTTTCTGGCTGGAAAAAATCGGTAAGAACGTTGAGCGCGATGAGCGCGATATTCAGCGTTTACAGGCGCT
TGGCTGGCGGGTGCTGATCGTCTGGGAGTGCGCCTTGCGCGGGCGAGCGAAATTGAGCGACGCGGCGTTGGCGGAGCGCC
TGGAAGAGTGGATCTGCGGCGGCGGCGCCAGCGCGCAGATTGACACCCAGGGGATTCATCTACTGGCGTGA

Upstream 100 bases:

>100_bases
TTTGGCAATTCCGTGGTGGTGCCGGTATTTGCCGCGGTAGCAAAGCTGCTGGAACCCAAAATTCACCAGGCGGTGGCGCT
GCGTCAGAGAGAGACGGTAG

Downstream 100 bases:

>100_bases
ATCTTCCGTTTTACACGGCGTGACCACCGCCCTGGCGGGAAACAGGTACTTACCCAGAGTCACCAGCACTACGGCAAACA
CAATCACACCCAGCGCAGCC

Product: very short patch repair protein

Products: DNA [C]

Alternate protein names: DNA mismatch endonuclease; V.EcoKDcm; Vsr mismatch endonuclease [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MADVHDKATRSKNMRAIATRDTAIEKRLAGLLSAQGITFHTQDATLPGKPDFVVNDYDCVIFTHGCFWHHHHCYLFKVPA
TRTAFWLEKIGKNVERDERDIQRLQALGWRVLIVWECALRGRAKLSDAALAERLEEWICGGGASAQIDTQGIHLLA

Sequences:

>Translated_156_residues
MADVHDKATRSKNMRAIATRDTAIEKRLAGLLSAQGITFHTQDATLPGKPDFVVNDYDCVIFTHGCFWHHHHCYLFKVPA
TRTAFWLEKIGKNVERDERDIQRLQALGWRVLIVWECALRGRAKLSDAALAERLEEWICGGGASAQIDTQGIHLLA
>Mature_155_residues
ADVHDKATRSKNMRAIATRDTAIEKRLAGLLSAQGITFHTQDATLPGKPDFVVNDYDCVIFTHGCFWHHHHCYLFKVPAT
RTAFWLEKIGKNVERDERDIQRLQALGWRVLIVWECALRGRAKLSDAALAERLEEWICGGGASAQIDTQGIHLLA

Specific function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=156, Percent_Identity=86.5384615384615, Blast_Score=286, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17594; Mature: 17463

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVHDKATRSKNMRAIATRDTAIEKRLAGLLSAQGITFHTQDATLPGKPDFVVNDYDCV
CCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCEEE
IFTHGCFWHHHHCYLFKVPATRTAFWLEKIGKNVERDERDIQRLQALGWRVLIVWECALR
EEECCEEEEECEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCC
GRAKLSDAALAERLEEWICGGGASAQIDTQGIHLLA
CCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEEC
>Mature Secondary Structure 
ADVHDKATRSKNMRAIATRDTAIEKRLAGLLSAQGITFHTQDATLPGKPDFVVNDYDCV
CCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCEEE
IFTHGCFWHHHHCYLFKVPATRTAFWLEKIGKNVERDERDIQRLQALGWRVLIVWECALR
EEECCEEEEECEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCC
GRAKLSDAALAERLEEWICGGGASAQIDTQGIHLLA
CCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2527357; 2198248; 9097040; 9278503; 1944537; 10871403; 10612397; 10360178 [H]