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The map label for this gene is gsiB [H]

Identifier: 194737769

GI number: 194737769

Start: 973140

End: 974678

Strand: Direct

Name: gsiB [H]

Synonym: SeSA_A1000

Alternate gene names: 194737769

Gene position: 973140-974678 (Clockwise)

Preceding gene: 194734810

Following gene: 194737415

Centisome position: 20.67

GC content: 53.09

Gene sequence:

>1539_bases
ATGACGCAATTTATTACGCATAAATGGCTGGTGGCGCTGGGACTGGCCTCATCCATTGCGGCTTTTCCGGCCCTGGCGGC
CAAAGATGTGGTGGTGGCGGTAGGGTCTAATTTCACCACGCTCGATCCGTATGACGCCAACGATACACTCTCGCAGGCGG
TGGCGAAGTCATTTTATCAGGGGCTGTTTGGCCTGGATAAAGACATGAAGGTAAAAAACGTCCTGGCGAAAGGCTATACC
GTTTCTGATGACGGTCTGACCTACACCATTACCCTACGGCAGGGGGTGAAATTTCAGGACGGCGCGGATTTTGATGCGGC
GGCGGTGAAAGCGAATCTCGATCGCGCCAGCAATCCGGATAACCACCTCAAGCGTTATAACCTGTATAAAAATATCGCAA
AAACGGAGGTTGTCGACCCGGCAACGGTAAAAATTACCCTGAAACAGCCGTTCTCTGCTTTCATTAATATTCTGGCGCAC
CCGGCGACGGCGATGATCTCGCCGCAAGCGCTGGAAAAATATGGCAAGGATATTGGTTTTCATCCGGTCGGTACCGGTCC
ATACCAGCTTGAAACCTGGAATCAGACCGACTTTGTCAAAGTGAAAAAGTTCGCCGGTTACTGGCAGCAGGGCTTACCCA
AGCTGGATTCTATTACCTGGCGTCCGGTTACTGACAACAATACCCGCGCGGCGATGCTGCAAACCGGCGAAGCGCAATTT
GCTTTTCCTATCCCTTATGAGCAGGCCGCGTTACTGGCGAAAAATAAAAACCTCGAACTGGTCGCCAGCCCGTCCATTAT
GCAGCGTTATATCAGTATGAATGTGACGCAGAAGCCGTTTGATAATCCTAAGGTGCGTGAGGCGTTGAATTACGCTATCA
ATCGTCAGGCGCTGGTTAAAGTGGCGTTTGCCGGATACGCCACGCCGGCGACGGGCGTGGTGCCGCCGTCGATAGCCTAT
GCGCAAAGCTATCAGCCGTGGCCCTATGATCCGGCTAAAGCGCGCGAACTGTTGAAAGAGGCGGGGTATCCAGACGGTTT
CAGTACCACGCTGTGGTCATCGCACAACCATAGCACCGCGCAAAAAGTGTTGCAGTTTACCCAACAGCAACTGGCGCAGA
TTGGCATTAAAGCCCGGATAACCGCGATGGACGCTGGCCAGCGCGCCGCGGAAGTTGAGGGCAAAGGGCAAAAAGAGAGC
GGCGTGCGAATGTTCTATACCGGCTGGTCGGCGTCGACGGGCGAAGCTGACTGGGCGTTATCGCCGCTCTTCGCATCACA
AAACTGGCCGCCGACGCAGTTTAATACCGCTTTTTACAGCAACAAGCAGGTCGACAGCGATTTGGCGGCGGCCTTAAAGA
CCAACGATCCGCAGGAGAAAACACGATTATACAAAGAGGCGCAGGATATTATCTGGAAAGAGTCGCCCTGGATACCGTTG
GTGGTGGAGAAATTGGTTTCTGCGCACAGTAAAAATTTGACCGGTTTCTGGATTATGCCGGATACCGGTTTCAGCTTTGA
CGATGCGGATTTGAAGTAA

Upstream 100 bases:

>100_bases
TACAGTTGATCGGGCCGGGACATTATGTTGCGCGGCCGCTGCAGGATAATGCGCTCTCGCGCTTATAACATTCAGACTGG
CAGGGTTTCAGGAGAACAAC

Downstream 100 bases:

>100_bases
GTAATGCGATGGGGCTGGATGGCGCGCGCGTTGTCCGCCATCCGGTAAAAGGTTCGTGTGATGCTTAACTATGTTCTCAA
GCGCCTGCTGGGATTAATTC

Product: glutathione-binding protein GsiB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTQFITHKWLVALGLASSIAAFPALAAKDVVVAVGSNFTTLDPYDANDTLSQAVAKSFYQGLFGLDKDMKVKNVLAKGYT
VSDDGLTYTITLRQGVKFQDGADFDAAAVKANLDRASNPDNHLKRYNLYKNIAKTEVVDPATVKITLKQPFSAFINILAH
PATAMISPQALEKYGKDIGFHPVGTGPYQLETWNQTDFVKVKKFAGYWQQGLPKLDSITWRPVTDNNTRAAMLQTGEAQF
AFPIPYEQAALLAKNKNLELVASPSIMQRYISMNVTQKPFDNPKVREALNYAINRQALVKVAFAGYATPATGVVPPSIAY
AQSYQPWPYDPAKARELLKEAGYPDGFSTTLWSSHNHSTAQKVLQFTQQQLAQIGIKARITAMDAGQRAAEVEGKGQKES
GVRMFYTGWSASTGEADWALSPLFASQNWPPTQFNTAFYSNKQVDSDLAAALKTNDPQEKTRLYKEAQDIIWKESPWIPL
VVEKLVSAHSKNLTGFWIMPDTGFSFDDADLK

Sequences:

>Translated_512_residues
MTQFITHKWLVALGLASSIAAFPALAAKDVVVAVGSNFTTLDPYDANDTLSQAVAKSFYQGLFGLDKDMKVKNVLAKGYT
VSDDGLTYTITLRQGVKFQDGADFDAAAVKANLDRASNPDNHLKRYNLYKNIAKTEVVDPATVKITLKQPFSAFINILAH
PATAMISPQALEKYGKDIGFHPVGTGPYQLETWNQTDFVKVKKFAGYWQQGLPKLDSITWRPVTDNNTRAAMLQTGEAQF
AFPIPYEQAALLAKNKNLELVASPSIMQRYISMNVTQKPFDNPKVREALNYAINRQALVKVAFAGYATPATGVVPPSIAY
AQSYQPWPYDPAKARELLKEAGYPDGFSTTLWSSHNHSTAQKVLQFTQQQLAQIGIKARITAMDAGQRAAEVEGKGQKES
GVRMFYTGWSASTGEADWALSPLFASQNWPPTQFNTAFYSNKQVDSDLAAALKTNDPQEKTRLYKEAQDIIWKESPWIPL
VVEKLVSAHSKNLTGFWIMPDTGFSFDDADLK
>Mature_511_residues
TQFITHKWLVALGLASSIAAFPALAAKDVVVAVGSNFTTLDPYDANDTLSQAVAKSFYQGLFGLDKDMKVKNVLAKGYTV
SDDGLTYTITLRQGVKFQDGADFDAAAVKANLDRASNPDNHLKRYNLYKNIAKTEVVDPATVKITLKQPFSAFINILAHP
ATAMISPQALEKYGKDIGFHPVGTGPYQLETWNQTDFVKVKKFAGYWQQGLPKLDSITWRPVTDNNTRAAMLQTGEAQFA
FPIPYEQAALLAKNKNLELVASPSIMQRYISMNVTQKPFDNPKVREALNYAINRQALVKVAFAGYATPATGVVPPSIAYA
QSYQPWPYDPAKARELLKEAGYPDGFSTTLWSSHNHSTAQKVLQFTQQQLAQIGIKARITAMDAGQRAAEVEGKGQKESG
VRMFYTGWSASTGEADWALSPLFASQNWPPTQFNTAFYSNKQVDSDLAAALKTNDPQEKTRLYKEAQDIIWKESPWIPLV
VEKLVSAHSKNLTGFWIMPDTGFSFDDADLK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=512, Percent_Identity=88.4765625, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=468, Percent_Identity=28.6324786324786, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787551, Length=535, Percent_Identity=27.4766355140187, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787762, Length=511, Percent_Identity=25.8317025440313, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789887, Length=508, Percent_Identity=26.1811023622047, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=24.3190661478599, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789397, Length=528, Percent_Identity=24.2424242424242, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081878, Length=503, Percent_Identity=20.2783300198807, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56581; Mature: 56449

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFITHKWLVALGLASSIAAFPALAAKDVVVAVGSNFTTLDPYDANDTLSQAVAKSFYQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHHHHHHHHHHH
GLFGLDKDMKVKNVLAKGYTVSDDGLTYTITLRQGVKFQDGADFDAAAVKANLDRASNPD
HHHCCCCCCHHHHHHHCCCEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCH
NHLKRYNLYKNIAKTEVVDPATVKITLKQPFSAFINILAHPATAMISPQALEKYGKDIGF
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCHHHHCCHHHHHHHCCCCCC
HPVGTGPYQLETWNQTDFVKVKKFAGYWQQGLPKLDSITWRPVTDNNTRAAMLQTGEAQF
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCEEEEEECCCCCE
AFPIPYEQAALLAKNKNLELVASPSIMQRYISMNVTQKPFDNPKVREALNYAINRQALVK
EECCCHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHH
VAFAGYATPATGVVPPSIAYAQSYQPWPYDPAKARELLKEAGYPDGFSTTLWSSHNHSTA
HHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCHHHH
QKVLQFTQQQLAQIGIKARITAMDAGQRAAEVEGKGQKESGVRMFYTGWSASTGEADWAL
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEE
SPLFASQNWPPTQFNTAFYSNKQVDSDLAAALKTNDPQEKTRLYKEAQDIIWKESPWIPL
CHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
VVEKLVSAHSKNLTGFWIMPDTGFSFDDADLK
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TQFITHKWLVALGLASSIAAFPALAAKDVVVAVGSNFTTLDPYDANDTLSQAVAKSFYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHHHHHHHHHHH
GLFGLDKDMKVKNVLAKGYTVSDDGLTYTITLRQGVKFQDGADFDAAAVKANLDRASNPD
HHHCCCCCCHHHHHHHCCCEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCH
NHLKRYNLYKNIAKTEVVDPATVKITLKQPFSAFINILAHPATAMISPQALEKYGKDIGF
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCHHHHCCHHHHHHHCCCCCC
HPVGTGPYQLETWNQTDFVKVKKFAGYWQQGLPKLDSITWRPVTDNNTRAAMLQTGEAQF
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCEEEEEECCCCCE
AFPIPYEQAALLAKNKNLELVASPSIMQRYISMNVTQKPFDNPKVREALNYAINRQALVK
EECCCHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHH
VAFAGYATPATGVVPPSIAYAQSYQPWPYDPAKARELLKEAGYPDGFSTTLWSSHNHSTA
HHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCHHHH
QKVLQFTQQQLAQIGIKARITAMDAGQRAAEVEGKGQKESGVRMFYTGWSASTGEADWAL
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEE
SPLFASQNWPPTQFNTAFYSNKQVDSDLAAALKTNDPQEKTRLYKEAQDIIWKESPWIPL
CHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
VVEKLVSAHSKNLTGFWIMPDTGFSFDDADLK
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA