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The map label for this gene is yfiE [H]

Identifier: 194737605

GI number: 194737605

Start: 2736869

End: 2737768

Strand: Reverse

Name: yfiE [H]

Synonym: SeSA_A2838

Alternate gene names: 194737605

Gene position: 2737768-2736869 (Counterclockwise)

Preceding gene: 194734376

Following gene: 194734721

Centisome position: 58.14

GC content: 51.11

Gene sequence:

>900_bases
ATGGATCTGCGTCGGTTTATTACGCTGAAAACGGTAGTGGAAGAGGGCTCGTTTCTGCGGGCTTCGCAAAAGCTGTGCTG
TACTCAGTCGACGGTAACTTTTCATATTCAACAGCTTGAACGTGAGTTTTCCCTGCAACTGTTCGAGAAAATAGGGCGCC
GAATGTGTCTGACCAATGAGGGGAAAAAGCTGATGCCGCATATCCATGAACTGACGCGTGTGATGGAGTCGATTCGTGAA
GCCGCAAGACAGGATGCGGAACCCGGCGGGGAGCTGCGCGTGGCGACAGGTGAAACCTTACTGGCGTACAAAATGCCGCA
GGTTTTACAACGATTTAAGCTGCGAGCGCCGAATGTCAGATTGTCGCTACAGTCGCTGAATTGCTATGTAATACGTGATG
CGTTGCTGAATGACGAGGTCGATCTGGGCGTTTTTTATCGCGTAGGTAATGATGATGCGCTCACGATGCAGCAACTGGGG
GAGCAGTCGCTGGCGCTGGTGGCCTCTCCACTGCTACAGGATGCTGATTTTACGCAGCCAGATCAGCATATTCCCTGTAG
CTTCATCATCAATGAACCGCAATGTGTCTTCCGCCAGCTGTTTGAAAGTACGCTACGGCAGAGGCGGATTACGCTGGAAA
ACACCATTGAACTGTGGAGTATTGAAAGCATTAAACAGTGTGTGGCTGCTAACCTGGGCATCAGCTTTTTACCACGTTTT
ACCGTTGAACGAGAACTTAGTACCGGTCAGTTGAAAGAGCTGCCGTTTGGCGCGCCGTCGCTATCGATCATGGCGCTGTG
CGCCCATCATGCCGGGAAAGCGGTCAGCCCGGCAATGCAGATATTTATGCAGTGTATGGAGGCGTGTTTTACCGTGGAAG
ATAAAAAAATGCCGGGTTAA

Upstream 100 bases:

>100_bases
GTCATCTCCTCTGTTTTAGCAGACAGTACCCGATATGAAATATTTAAAAAAATGAATGTTTTTTAATTCTGAATTCGAGA
TTTTCGATAGGTTGGTGGTT

Downstream 100 bases:

>100_bases
CGAACCCGGCAGTATCATCACTCTTCGCTGGATTGCTCCTGCATTTTCGTACCGCTTGGCTTGCGGCGCTTACCGATATT
TTTTGTATCGCGGTGACGTT

Product: LysR substrate binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
EQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
TVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG

Sequences:

>Translated_299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
EQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
TVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
>Mature_299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
EQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
TVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=291, Percent_Identity=78.0068728522337, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=22.2996515679443, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788748, Length=268, Percent_Identity=23.134328358209, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=157, Percent_Identity=29.9363057324841, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=23.8866396761134, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693105, Length=297, Percent_Identity=22.5589225589226, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33897; Mature: 33897

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GKKLMPHIHELTRVMESIREAARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLGEQSLALVASPLLQDADFTQP
EEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCC
DQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCH
TVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
HHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GKKLMPHIHELTRVMESIREAARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLGEQSLALVASPLLQDADFTQP
EEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCC
DQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCH
TVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
HHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]