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The map label for this gene is metL [H]

Identifier: 194737573

GI number: 194737573

Start: 4187957

End: 4190389

Strand: Direct

Name: metL [H]

Synonym: SeSA_A4318

Alternate gene names: 194737573

Gene position: 4187957-4190389 (Clockwise)

Preceding gene: 194736514

Following gene: 194735507

Centisome position: 88.93

GC content: 58.73

Gene sequence:

>2433_bases
ATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAACTGCATAAATTTGGCGGCAGTAGTCTGGCGGATGTGAAGTG
TTACCTGCGTGTCGCAGGCATTATGGCGGAGTATTCGCAGCCTGACGATATGATGGTAGTGTCCGCCGCAGGCAGTACCA
CCAACCAGTTGATTAGCTGGCTCAAGTTAAGCCAGACCGATCGCCTTTCTGCGCATCAGGTATTACAGACGTTACGCCGC
TACCAGTGCGATCTGATTAGCGGGCTGCTTCCCGCCGACGCGGCAGATGACTTAACCAGCGCTTTTATCAGCGATCTGGA
ACGTCTTGCCGCCTTGCTCGACGGCGGCGTAACGGATGCCGTCTATGCCGAGATTGTCGGACACGGCGAAATTTGGTCGG
CGAGATTGATGTCCGCCGTCCTGAATCAGCAGGGGCTGGATGCCGCCTGGCTGGACGCGCGCGCATTTTTACGCGCCGAA
CGCGCCGCACAGCCGCAGGTTGATGAAGGGCTATCTTATCCTTTATTGCAACAGCTACTGGCGCAGCATCCTGGCAAACG
TCTGGTCGTGACCGGCTTTATCAGCCGCAATCATGACGGCGAAACGGTCCTGTTAGGCCGGAACGGTTCCGACTATTCCG
CCACGCAAATCGGCGCGCTGGCGGGCGTTTCCCGCGTGACCATCTGGAGCGATGTCGCGGGCGTTTATAGTGCCGACCCG
CGCAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTTTGGACGAAGCCAGCGAGCTGGCGCGTCTGGCGGCGCCTGT
TCTGCACGCCCGCACGCTACAGCCAGTGTCCGGCAGCGATATCGATTTGCAGTTGCGCTGTAGCTACACGCCGGATCAAG
GTTCAACCCGAATTGAACGGGTGCTGGCTTCCGGGACGGGCGCGCGTATCGTGACCAGTCACGATGATATCTGTCTGATT
GAGTTTCAGGTGCCCGCCAGCCAGGATTTCAGGCTGGCGCATAAAGAGCTCGACCACATTTTAAAACGGGCGCAGGTGCG
TCCGCTGGCGGTGGGCGTGCATCGCGATCGCCAGTTATTACAGTTCTGCTATACCGCCGAGGTAGCGGATAGCGTACTGA
AACTCCTCGACGATGTCGGGCTGCCGGGCGAATTGCGCCTGCGGCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCGGGC
GTTACTCGCAATCCGCTACACTGCCATCGCTTCTGGCAGCAGTTGAAAGGTCAGCCAGTGGAGTTTACTTGGCAGTCGGA
GGAGGGCATCAGTCTGGTGGCGGTGCTGCGTACCGGCCCGACTGAGAGCCTGATTCAGGGGCTGCACCAGTCTGTTTTCC
GCGCCGAAAAACGCATTGGCCTGATGCTGTTCGGTAAGGGGAACATCGGCTCCCGTTGGCTGGAACTATTTGCCCGTGAA
CAAAGCACGCTTTCCGCGCGTACTGGTTTTGAATTTGTGCTGGCGGGCGTGGTGGATAGCCGCCGTAGCCTGTTGAATTA
TGAAGGGCTGGACGCCAGCCGCGCGCTGGCATTCTTTGATGATGAAGCCGTTGAGCAGGACGAAGAGTCGCTATTCCTGT
GGATGCGTGCGCATCCGTATGATGATTTAGTGGTGCTGGATGTGACAGCAAGCGAGCAACTGGCCGATCAGTACCTTGAT
TTCGCCAGCCACGGTTTTCATGTGATTAGCGCCAATAAACTGGCGGGCGCAAGCGCCAGCGATAAGTATCGCCAGATTCA
TGACGCGTTTGAAAAAACCGGGCGTTACTGGCTGTATAACGCCACCGTGGGCGCGGGATTGCCGATCAACCACACTGTGC
GGGATCTGATTGACAGCGGCGATACGATTCTCTCGATCAGCGGGATCTTCTCCGGGACGCTATCCTGGCTGTTCCTGCAA
TTTGACGGTACGGTGCCTTTCACCGATCTGGTGGATCAGGCCTGGCAGCAGGGGTTGACGGAACCCGATCCGCGCGTCGA
TCTTTCCGGAAAAGATGTCATGCGTAAGCTGGTGATTCTGGCGCGCGAGGCGGGATACGACATCGAGCCGGATCAGGTGC
GTGTAGAATCGTTGGTGCCGGCGCATTGTGAAGAAGGGTCTATCGATCACTTTTTCGAAAACGGCGACGCGCTGAATGAA
CAGATGGTACAGCGTCTGGAAGCAGCCCGCGAACTGGGGCTGGTGTTGCGTTATGTGGCGCGTTTCGACGCCAATGGCAA
AGCGCGCGTCGGCGTTGAAGCGGTGCGTCCGGAGCATCCGCTGGCGGCGCTGCTGCCGTGCGATAATGTCTTTGCTATTG
AAAGCCGCTGGTATCGCGATAACCCGCTGGTGATCCGTGGGCCGGGCGCGGGTCGCGATGTGACCGCCGGGGCGATTCAG
TCTGACATCAACCGCCTGGCGCAGCTATTGTAA

Upstream 100 bases:

>100_bases
TCTCCGAAACGCTACTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGG
GCTGCAAACAAGGGGTAAAC

Downstream 100 bases:

>100_bases
TACGCCTGCCTGCCGGATAGCGGCTTCGCCAGATCCGGCAGCGGGCAAACCGGGGTATCTGTTTATTCTTCGTCAGATGC
GTTTTCTGCATTGGCGACGA

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLISWLKLSQTDRLSAHQVLQTLRR
YQCDLISGLLPADAADDLTSAFISDLERLAALLDGGVTDAVYAEIVGHGEIWSARLMSAVLNQQGLDAAWLDARAFLRAE
RAAQPQVDEGLSYPLLQQLLAQHPGKRLVVTGFISRNHDGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDICLI
EFQVPASQDFRLAHKELDHILKRAQVRPLAVGVHRDRQLLQFCYTAEVADSVLKLLDDVGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSEEGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLMLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLNYEGLDASRALAFFDDEAVEQDEESLFLWMRAHPYDDLVVLDVTASEQLADQYLD
FASHGFHVISANKLAGASASDKYRQIHDAFEKTGRYWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGTVPFTDLVDQAWQQGLTEPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSIDHFFENGDALNE
QMVQRLEAARELGLVLRYVARFDANGKARVGVEAVRPEHPLAALLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL

Sequences:

>Translated_810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLISWLKLSQTDRLSAHQVLQTLRR
YQCDLISGLLPADAADDLTSAFISDLERLAALLDGGVTDAVYAEIVGHGEIWSARLMSAVLNQQGLDAAWLDARAFLRAE
RAAQPQVDEGLSYPLLQQLLAQHPGKRLVVTGFISRNHDGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDICLI
EFQVPASQDFRLAHKELDHILKRAQVRPLAVGVHRDRQLLQFCYTAEVADSVLKLLDDVGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSEEGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLMLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLNYEGLDASRALAFFDDEAVEQDEESLFLWMRAHPYDDLVVLDVTASEQLADQYLD
FASHGFHVISANKLAGASASDKYRQIHDAFEKTGRYWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGTVPFTDLVDQAWQQGLTEPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSIDHFFENGDALNE
QMVQRLEAARELGLVLRYVARFDANGKARVGVEAVRPEHPLAALLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL
>Mature_809_residues
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLISWLKLSQTDRLSAHQVLQTLRRY
QCDLISGLLPADAADDLTSAFISDLERLAALLDGGVTDAVYAEIVGHGEIWSARLMSAVLNQQGLDAAWLDARAFLRAER
AAQPQVDEGLSYPLLQQLLAQHPGKRLVVTGFISRNHDGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPR
KVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDICLIE
FQVPASQDFRLAHKELDHILKRAQVRPLAVGVHRDRQLLQFCYTAEVADSVLKLLDDVGLPGELRLRQGLALVAMVGAGV
TRNPLHCHRFWQQLKGQPVEFTWQSEEGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLMLFGKGNIGSRWLELFAREQ
STLSARTGFEFVLAGVVDSRRSLLNYEGLDASRALAFFDDEAVEQDEESLFLWMRAHPYDDLVVLDVTASEQLADQYLDF
ASHGFHVISANKLAGASASDKYRQIHDAFEKTGRYWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF
DGTVPFTDLVDQAWQQGLTEPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSIDHFFENGDALNEQ
MVQRLEAARELGLVLRYVARFDANGKARVGVEAVRPEHPLAALLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQS
DINRLAQLL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=810, Percent_Identity=94.0740740740741, Blast_Score=1561, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=828, Percent_Identity=30.7971014492754, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI1790455, Length=288, Percent_Identity=31.25, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322599, Length=370, Percent_Identity=34.3243243243243, Blast_Score=163, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6320893, Length=474, Percent_Identity=23.6286919831224, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88913; Mature: 88782

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLISW
CCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVLQTLRRYQCDLISGLLPADAADDLTSAFISDLERLAALLDGGVTDA
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VYAEIVGHGEIWSARLMSAVLNQQGLDAAWLDARAFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
AQHPGKRLVVTGFISRNHDGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDICLIEFQVPASQDFRLAHKELDHILKRAQVRPLAVGVHRDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
QFCYTAEVADSVLKLLDDVGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSEEGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLMLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLNYEGLDASRALAFFDDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASEQLADQYLDFASHGFHVISANKLAGASASDKYRQIHDAFEKTGRYWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGTVPFTDLVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSIDHFFENGDALNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEHHHHCCCCCCCCCCHHHHCCCCHHHH
QMVQRLEAARELGLVLRYVARFDANGKARVGVEAVRPEHPLAALLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEECCCCCCCEEEECCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLISW
CCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVLQTLRRYQCDLISGLLPADAADDLTSAFISDLERLAALLDGGVTDA
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VYAEIVGHGEIWSARLMSAVLNQQGLDAAWLDARAFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
AQHPGKRLVVTGFISRNHDGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDICLIEFQVPASQDFRLAHKELDHILKRAQVRPLAVGVHRDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
QFCYTAEVADSVLKLLDDVGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSEEGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLMLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLNYEGLDASRALAFFDDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASEQLADQYLDFASHGFHVISANKLAGASASDKYRQIHDAFEKTGRYWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGTVPFTDLVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSIDHFFENGDALNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEHHHHCCCCCCCCCCHHHHCCCCHHHH
QMVQRLEAARELGLVLRYVARFDANGKARVGVEAVRPEHPLAALLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEECCCCCCCEEEECCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]