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The map label for this gene is rfaI [H]

Identifier: 194737566

GI number: 194737566

Start: 3788872

End: 3789885

Strand: Reverse

Name: rfaI [H]

Synonym: SeSA_A3917

Alternate gene names: 194737566

Gene position: 3789885-3788872 (Counterclockwise)

Preceding gene: 194737924

Following gene: 194734939

Centisome position: 80.48

GC content: 37.67

Gene sequence:

>1014_bases
ATGAGCAGAAAATATTTTGAAGAAGAAGTCATTCAACAGACTTTAGATTATAACTATGCACAACATAGTGATGCTGATAA
ATTTAATATAGCTTATGGGATTGATAAAAACTTTCTTTTTGGCTGTGGTGTCTCTATTGCATCGGTTCTCCTTGCTAACC
CAGAGAAGGCGTTAGCTTTCCATGTTTTTACCGATTTCTTTGACTCTGAAGACCAGCAGCGATTTGAGGCATTAGCAAAA
CAGTACGCTACGCAGATTGTTGTTTACCTAATCGACTGTGAGCGCTTAAAATCGCTACCCAGTACCAAAAACTGGACCTA
TGCAACATACTTTAGATTCATTATCGCCGATTATTTTTCAGATAAAACAGATAGAGTACTTTATCTGGATGCTGATATTG
CATGTAAGGGGAGTATTCAGGAGCTTATTGAGCTTAACTTCGCTGAAAATGAGATTGCGGCTGTCGTTGCTGAAGGCGAG
TTGGAATGGTGGACTAAGCGCTCGGTTAGCCTGGCAACGCCTGGGCTGGTTTCTGGCTATTTTAATGCCGGTTTTATTTT
AATTAACATACCTCTTTGGACCGCAGAAAATATCTCTAAGAAAGCGATTGAAATGCTAAAAGATCCGGAGGTAGTACAGC
GCATAACGCACCTTGATCAGGATGTATTAAATATATTGTTAGTGAATAAAGCACGTTTTGTTGATAAAAAATTTAATACA
CAATTTAGTCTTAACTATGAATTAAAAGATTCAGTTATTAATCCAGTTGATGCTGAGACTGTATTTGTTCATTATATCGG
ACCAACGAAGCCCTGGCATAGTTGGGGGGCTTATCCTGTGTCACAATATTTTTTACAGGCTAAGAGCAACTCACCATGGT
CTCATTGTGCACTTTTAAATCCAGTCACTAGTCATCAGTTACGTTATGCGGCAAAGCATATGTTTAATCAGAAGCATTAT
ACTTCGGGTATAAATTATTATATAGCCTACTTTAAACGTAAACTTCTTGAATAA

Upstream 100 bases:

>100_bases
AGCATGATATTATTCCCGGCACGATTGAGAGATTTTACGATGTGCTATATTTTAAAAATTTTAATAATGCAATATTCTCG
AAATTACAAAAGTGATCACT

Downstream 100 bases:

>100_bases
AACCCATAGGTGATGTAATGGATTCATTTCCTGAGATAGAAATAGCTGAATATAAAGTTTTTGATGAAAGTAATAATAAT
AATGATGATAACGTATTAAA

Product: lipopolysaccharide 1,3-galactosyltransferase

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSRKYFEEEVIQQTLDYNYAQHSDADKFNIAYGIDKNFLFGCGVSIASVLLANPEKALAFHVFTDFFDSEDQQRFEALAK
QYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIELNFAENEIAAVVAEGE
LEWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHY
TSGINYYIAYFKRKLLE

Sequences:

>Translated_337_residues
MSRKYFEEEVIQQTLDYNYAQHSDADKFNIAYGIDKNFLFGCGVSIASVLLANPEKALAFHVFTDFFDSEDQQRFEALAK
QYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIELNFAENEIAAVVAEGE
LEWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHY
TSGINYYIAYFKRKLLE
>Mature_336_residues
SRKYFEEEVIQQTLDYNYAQHSDADKFNIAYGIDKNFLFGCGVSIASVLLANPEKALAFHVFTDFFDSEDQQRFEALAKQ
YATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIELNFAENEIAAVVAEGEL
EWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNTQ
FSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCALLNPVTSHQLRYAAKHMFNQKHYT
SGINYYIAYFKRKLLE

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=337, Percent_Identity=54.3026706231454, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1790056, Length=324, Percent_Identity=34.5679012345679, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 38885; Mature: 38754

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKYFEEEVIQQTLDYNYAQHSDADKFNIAYGIDKNFLFGCGVSIASVLLANPEKALAF
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHCCCCHHHHH
HVFTDFFDSEDQQRFEALAKQYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHC
DKTDRVLYLDADIACKGSIQELIELNFAENEIAAVVAEGELEWWTKRSVSLATPGLVSGY
CCCCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHC
FNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
CCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCALLN
EEEEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHEEECC
PVTSHQLRYAAKHMFNQKHYTSGINYYIAYFKRKLLE
CHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRKYFEEEVIQQTLDYNYAQHSDADKFNIAYGIDKNFLFGCGVSIASVLLANPEKALAF
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHCCCCHHHHH
HVFTDFFDSEDQQRFEALAKQYATQIVVYLIDCERLKSLPSTKNWTYATYFRFIIADYFS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHC
DKTDRVLYLDADIACKGSIQELIELNFAENEIAAVVAEGELEWWTKRSVSLATPGLVSGY
CCCCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHHHC
FNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNILLVNKARFVDKKFNT
CCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QFSLNYELKDSVINPVDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCALLN
EEEEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHEEECC
PVTSHQLRYAAKHMFNQKHYTSGINYYIAYFKRKLLE
CHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]