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The map label for this gene is eptA [H]

Identifier: 194737369

GI number: 194737369

Start: 4424972

End: 4426615

Strand: Reverse

Name: eptA [H]

Synonym: SeSA_A4548

Alternate gene names: 194737369

Gene position: 4426615-4424972 (Counterclockwise)

Preceding gene: 194736542

Following gene: 194734993

Centisome position: 94.0

GC content: 49.57

Gene sequence:

>1644_bases
ATGTTAAAGCGCTTTCTTAAAAGACCTGTTCTTGGGCAAATCGCCTGGCTTCTGCTTTTTTCCTTTTATATTGCCGTCTG
CCTGAACATTGCGTTCTACAAGCAGGTACTACAAGACCTACCGTTAAACTCGCTGCGCAATGTGCTGGTGTTTATTTCCA
TGCCGGTCGTCGCGTTTAGCGTGGTCAATAGTGTGCTGACGCTGGCCTCATTTATTTGGCTAAACCGACTGCTGGCCTGC
GTTTTTATTCTGGTCGGCGCTGCCGCGCAGTATTTTATTTTGACTTACGGCATCATCATCGATCGCTCCATGATCGCCAA
TATGATGGATACCACGCCTGCGGAAACCTTTGCGCTGATGACGCCGCAAATGGTGCTGACGCTGGGATTAAGCGGCGTTC
TGGCCGCCGTGATTGCCTTCTGGGTCAAAATCCGCCCGGCGACGCCGCGCTTACGTAGCGGGCTTTACCGCCTGGCCAGC
GTCCTGATCTCTATTTTATTAGTTATTCTGGTCGCCGCCTTTTTCTACAAAGATTACGCCTCGCTATTTCGAAATAATAA
ACAGTTGATCAAAGCGTTAAGCCCATCGAACAGTATTGTCGCCAGTTGGTCATGGTATTCGCATCAACGGCTGGCGAATT
TGCCGCTGGTACGCATTGGCGAGGATGCCCATCGCAATCCATTAATGCTGAAAGGCGATCGCAAAAACCTGACGATTCTT
ATCGTTGGCGAAACCTCGCGCGGCGATGATTTCTCTCTTGGCGGCTATCCGCGCGACACCAATCCGCGGCTGGCGAAAGA
CGATGTAATCTATTTCCCGCATACCACCTCTTGCGGTACGGCGACCGCGATCTCCGTTCCCTGCATGTTTTCTGATATGC
CGCGCAAACATTATGACGAAGAGCTGGCGCATCACCAGGAAGGGCTGCTGGATATTATCCAGCGCGCGGGGATTAACGTA
CTGTGGAATGATAATGACGGCGGCTGTAAAGGCGCATGCGATCGCGTTCCGCATCAAAATGTCACAGAGCTAAACCTGCC
CGGACAATGTATCGACGGCGAGTGCTACGATGAGGTGTTATTCCACGGTCTGGAAGACTATATCGACCATTTGAAAGGCG
ATGGCGTTATTGTACTGCATACCATCGGCAGCCACGGCCCAACCTATTACAACCGTTATCCACCGCAGTTTAAAAAATTT
ACCCCAACCTGTGACACTAACGAAATTCAGAACTGTTCCCAACAGCAGTTGATCAACACCTATGATAATACCGTGCTGTA
CGTGGACTATATTGTAGATAAAGCCATTAATCTGCTGAAATCTCATCAGGATAAATTCACAACCAGCCTGGTCTATCTTT
CCGATCACGGCGAATCATTGGGTGAAAATGGCGTTTATTTACACGGTCTGCCGTATTCTATTGCGCCGGATACGCAAAAA
CATGTCCCGATGCTCATTTGGCTTTCTAAAGATTATCAACAGCGTTATCAGGTGGATCAGGCCTGCCTGCAAAAACGGGC
GAGCACGCTGGATTATTCACAGGACAATCTTTTCTCAACCATGCTGGGATTAACCGGCGTACAAACGACGTATTACCAGG
CCGCAGATGATATTCTGCAACCGTGCAGGAGACTAAGCGAATGA

Upstream 100 bases:

>100_bases
ATAGCGCAGGTTATCCGGCCGCCACCAACATTAAGTTCTTAAGGTTCACTTAATTTTACTTTGTCACGATTAGCGTCACC
GAATCGATGGACGCATCAAC

Downstream 100 bases:

>100_bases
AGATACTGATTGTTGAAGACGACACGCTATTATTACAGGGGTTAATACTCGCCGCGCAAACCGAAGGCTATGCGTGTGAT
GGCGTTTCGACAGCGCGTGC

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLKRFLKRPVLGQIAWLLLFSFYIAVCLNIAFYKQVLQDLPLNSLRNVLVFISMPVVAFSVVNSVLTLASFIWLNRLLAC
VFILVGAAAQYFILTYGIIIDRSMIANMMDTTPAETFALMTPQMVLTLGLSGVLAAVIAFWVKIRPATPRLRSGLYRLAS
VLISILLVILVAAFFYKDYASLFRNNKQLIKALSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMLKGDRKNLTIL
IVGETSRGDDFSLGGYPRDTNPRLAKDDVIYFPHTTSCGTATAISVPCMFSDMPRKHYDEELAHHQEGLLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTELNLPGQCIDGECYDEVLFHGLEDYIDHLKGDGVIVLHTIGSHGPTYYNRYPPQFKKF
TPTCDTNEIQNCSQQQLINTYDNTVLYVDYIVDKAINLLKSHQDKFTTSLVYLSDHGESLGENGVYLHGLPYSIAPDTQK
HVPMLIWLSKDYQQRYQVDQACLQKRASTLDYSQDNLFSTMLGLTGVQTTYYQAADDILQPCRRLSE

Sequences:

>Translated_547_residues
MLKRFLKRPVLGQIAWLLLFSFYIAVCLNIAFYKQVLQDLPLNSLRNVLVFISMPVVAFSVVNSVLTLASFIWLNRLLAC
VFILVGAAAQYFILTYGIIIDRSMIANMMDTTPAETFALMTPQMVLTLGLSGVLAAVIAFWVKIRPATPRLRSGLYRLAS
VLISILLVILVAAFFYKDYASLFRNNKQLIKALSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMLKGDRKNLTIL
IVGETSRGDDFSLGGYPRDTNPRLAKDDVIYFPHTTSCGTATAISVPCMFSDMPRKHYDEELAHHQEGLLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTELNLPGQCIDGECYDEVLFHGLEDYIDHLKGDGVIVLHTIGSHGPTYYNRYPPQFKKF
TPTCDTNEIQNCSQQQLINTYDNTVLYVDYIVDKAINLLKSHQDKFTTSLVYLSDHGESLGENGVYLHGLPYSIAPDTQK
HVPMLIWLSKDYQQRYQVDQACLQKRASTLDYSQDNLFSTMLGLTGVQTTYYQAADDILQPCRRLSE
>Mature_547_residues
MLKRFLKRPVLGQIAWLLLFSFYIAVCLNIAFYKQVLQDLPLNSLRNVLVFISMPVVAFSVVNSVLTLASFIWLNRLLAC
VFILVGAAAQYFILTYGIIIDRSMIANMMDTTPAETFALMTPQMVLTLGLSGVLAAVIAFWVKIRPATPRLRSGLYRLAS
VLISILLVILVAAFFYKDYASLFRNNKQLIKALSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMLKGDRKNLTIL
IVGETSRGDDFSLGGYPRDTNPRLAKDDVIYFPHTTSCGTATAISVPCMFSDMPRKHYDEELAHHQEGLLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTELNLPGQCIDGECYDEVLFHGLEDYIDHLKGDGVIVLHTIGSHGPTYYNRYPPQFKKF
TPTCDTNEIQNCSQQQLINTYDNTVLYVDYIVDKAINLLKSHQDKFTTSLVYLSDHGESLGENGVYLHGLPYSIAPDTQK
HVPMLIWLSKDYQQRYQVDQACLQKRASTLDYSQDNLFSTMLGLTGVQTTYYQAADDILQPCRRLSE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=547, Percent_Identity=82.2669104204753, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI87082286, Length=480, Percent_Identity=25.8333333333333, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082223, Length=441, Percent_Identity=24.4897959183673, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61619; Mature: 61619

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFLKRPVLGQIAWLLLFSFYIAVCLNIAFYKQVLQDLPLNSLRNVLVFISMPVVAFS
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VVNSVLTLASFIWLNRLLACVFILVGAAAQYFILTYGIIIDRSMIANMMDTTPAETFALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TPQMVLTLGLSGVLAAVIAFWVKIRPATPRLRSGLYRLASVLISILLVILVAAFFYKDYA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFRNNKQLIKALSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMLKGDRKNLTIL
HHHHCCHHHHHHHCCCCCEEEEECHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEE
IVGETSRGDDFSLGGYPRDTNPRLAKDDVIYFPHTTSCGTATAISVPCMFSDMPRKHYDE
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECCHHHCCCCHHHHHH
ELAHHQEGLLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTELNLPGQCIDGECYDEVL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
FHGLEDYIDHLKGDGVIVLHTIGSHGPTYYNRYPPQFKKFTPTCDTNEIQNCSQQQLINT
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCHHHHHHH
YDNTVLYVDYIVDKAINLLKSHQDKFTTSLVYLSDHGESLGENGVYLHGLPYSIAPDTQK
HCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
HVPMLIWLSKDYQQRYQVDQACLQKRASTLDYSQDNLFSTMLGLTGVQTTYYQAADDILQ
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
PCRRLSE
HHHHHCC
>Mature Secondary Structure
MLKRFLKRPVLGQIAWLLLFSFYIAVCLNIAFYKQVLQDLPLNSLRNVLVFISMPVVAFS
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VVNSVLTLASFIWLNRLLACVFILVGAAAQYFILTYGIIIDRSMIANMMDTTPAETFALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TPQMVLTLGLSGVLAAVIAFWVKIRPATPRLRSGLYRLASVLISILLVILVAAFFYKDYA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFRNNKQLIKALSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMLKGDRKNLTIL
HHHHCCHHHHHHHCCCCCEEEEECHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEE
IVGETSRGDDFSLGGYPRDTNPRLAKDDVIYFPHTTSCGTATAISVPCMFSDMPRKHYDE
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECCHHHCCCCHHHHHH
ELAHHQEGLLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTELNLPGQCIDGECYDEVL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
FHGLEDYIDHLKGDGVIVLHTIGSHGPTYYNRYPPQFKKFTPTCDTNEIQNCSQQQLINT
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCHHHHHHH
YDNTVLYVDYIVDKAINLLKSHQDKFTTSLVYLSDHGESLGENGVYLHGLPYSIAPDTQK
HCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
HVPMLIWLSKDYQQRYQVDQACLQKRASTLDYSQDNLFSTMLGLTGVQTTYYQAADDILQ
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
PCRRLSE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]