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The map label for this gene is dusB [H]

Identifier: 194737360

GI number: 194737360

Start: 3453477

End: 3454442

Strand: Direct

Name: dusB [H]

Synonym: SeSA_A3576

Alternate gene names: 194737360

Gene position: 3453477-3454442 (Clockwise)

Preceding gene: 194734237

Following gene: 194737822

Centisome position: 73.34

GC content: 53.62

Gene sequence:

>966_bases
ATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACTGACAGACCTTTCCGGAC
GCTGTGCTATGAGATGGGAGCAGGATTGACAGTATCCGAGATGATGTCTTCTAACCCGCAAGTGTGGGAAAGCGATAAAT
CTCGTTTACGGATGGTGCACGTTGATGAGCCAGGTATTCGCACGGTGCAAATTGCCGGTAGCGACCCTGTTGAGATGGCC
GATGCCGCACGTATTAACGTGGAAAGCGGCGCCCAGATTATTGATATCAATATGGGGTGCCCGGCGAAGAAAGTGAATCG
TAAGCTGGCAGGTTCAGCCCTCTTGCAGTACCCGGATTTAGTGAAGTCTATACTCATCGGGGTCGTAAACGCAGTGGACG
TTCCTGTGACTCTCAAGATTCGCACCGGCTGGGCGCCGGAACACCGTAACTGCGTAGAGATTGCCCAACTGGCTGAAGAT
TGTGGTATTCAGGCTCTGACCATCCATGGCCGCACCCGCGCCTGTTTGTTCAACGGAGAAGCTGAATACGACAGTATTCG
GGCAGTTAAGCAGAAAGTTTCCATTCCGATTATCGCGAATGGCGACATTACTAACCCGCATAAAGCCAGAGCTGTTCTCG
ACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTAT
CTGGACACTGGGGAGCTGCTTCCGCCGCTGCCGCTGGCAGAGGTAAAGCGCTTGCTTTGCACGCATGTTCGGGAACTGCA
TGACTTTTACGGTCAGGCAAAAGGGTACCGAATCGCGCGTAAACACGTCTCCTGGTATCTCCAGGAGCACGCTCCAGACG
ACCAGTTTCGGCGCACATTCAACGCTATTGAGGACGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTT
GCGTAA

Upstream 100 bases:

>100_bases
ATCTACAACAGTGGATTGTTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACA
GTATGGTCATTTCTTAACTC

Downstream 100 bases:

>100_bases
ACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTC
AGGATCAGGTAACCCAAAAA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A

Sequences:

>Translated_321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A
>Mature_321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=31.1203319502075, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI31742496, Length=320, Percent_Identity=30.625, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=32.7800829875519, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=30.2788844621514, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=30.4347826086957, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=96.2616822429907, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=241, Percent_Identity=31.5352697095436, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=30.1587301587302, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=199, Percent_Identity=35.1758793969849, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=32.9268292682927, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=270, Percent_Identity=30.3703703703704, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=30.1310043668122, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=31.2757201646091, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=33.739837398374, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=32.1888412017167, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=32.1888412017167, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35847; Mature: 35847

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
VDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
ECCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCC
AEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
VDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
ECCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCC
AEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]