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The map label for this gene is prgK

Identifier: 194737310

GI number: 194737310

Start: 2922202

End: 2922960

Strand: Reverse

Name: prgK

Synonym: SeSA_A3024

Alternate gene names: 194737310

Gene position: 2922960-2922202 (Counterclockwise)

Preceding gene: 194737166

Following gene: 194735518

Centisome position: 62.07

GC content: 46.11

Gene sequence:

>759_bases
ATGATTCGTCGATATCTATATACCTTTCTGCTGGTAATGACCCTTGCCGGCTGTAAGGATAAGGATCTTTTAAAAGGACT
GGACCAGGAACAGGCTAATGAGGTCATTGCCGTTCTGCAAATGCACAATATAGAGGCGAATAAAATTGATAGCGGAAAAT
TGGGCTATAGCATTACCGTTGCTGAGCCTGATTTTACCGCTGCGGTGTACTGGATTAAAACTTATCAGCTTCCTCCCCGG
CCGCGGGTGGAAATAGCGCAGATGTTCCCGGCGGATTCGCTGGTATCGTCTCCGCGAGCTGAAAAGGCCAGGTTATATTC
GGCTATTGAACAGCGACTGGAACAGTCATTACAGACGATGGAGGGCGTGCTCTCCGCCAGGGTCCATATTAGTTATGATA
TTGATGCTGGTGAAAATGGCCGCCCGCCAAAACCTGTTCATCTGTCGGCATTAGCCGTATATGAACGAGGTTCGCCGCTT
GCGCATCAGATCAGCGATATCAAGCGTTTCTTAAAGAATAGTTTTGCCGATGTGGATTATGACAACATTTCTGTTGTGTT
GTCAGAACGTTCTGATGCCCAATTACAGGCTCCCGGCACACCAGTAAAACGTAATTCTTTTGCAACCAGTTGGATTGTGT
TGATTATTTTGTTATCCGTGATGTCAGCAGGCTTTGGCGTCTGGTATTACAAAAACCATTATGCCCGCAATAAGAAAGGC
ATAACGGCTGATGATAAGGCGAAATCGTCAAATGAATAG

Upstream 100 bases:

>100_bases
CTATTTCGCAAGAGATGATTTCAGATTATAACCTGTATGTTTCTATGGTAAGTACCCTTACTCGTAAAGGAGTCGGGGCT
GTTGAAACGCTATTACGCTC

Downstream 100 bases:

>100_bases
GCAGCCATTACCCATTATCTGGCAAAGAATCATTTTTGATCCGTTATCGTATATCCATCCTCAGCGGTTGCAGATAGCGC
CGGAAATGATTGTCAGACCC

Product: type III secretion apparatus lipoprotein, YscJ/HrcJ family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIRRYLYTFLLVMTLAGCKDKDLLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITVAEPDFTAAVYWIKTYQLPPR
PRVEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTMEGVLSARVHISYDIDAGENGRPPKPVHLSALAVYERGSPL
AHQISDIKRFLKNSFADVDYDNISVVLSERSDAQLQAPGTPVKRNSFATSWIVLIILLSVMSAGFGVWYYKNHYARNKKG
ITADDKAKSSNE

Sequences:

>Translated_252_residues
MIRRYLYTFLLVMTLAGCKDKDLLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITVAEPDFTAAVYWIKTYQLPPR
PRVEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTMEGVLSARVHISYDIDAGENGRPPKPVHLSALAVYERGSPL
AHQISDIKRFLKNSFADVDYDNISVVLSERSDAQLQAPGTPVKRNSFATSWIVLIILLSVMSAGFGVWYYKNHYARNKKG
ITADDKAKSSNE
>Mature_252_residues
MIRRYLYTFLLVMTLAGCKDKDLLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITVAEPDFTAAVYWIKTYQLPPR
PRVEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTMEGVLSARVHISYDIDAGENGRPPKPVHLSALAVYERGSPL
AHQISDIKRFLKNSFADVDYDNISVVLSERSDAQLQAPGTPVKRNSFATSWIVLIILLSVMSAGFGVWYYKNHYARNKKG
ITADDKAKSSNE

Specific function: Required for invasion of epithelial cells. Could be involved in protein secretion

COG id: COG4669

COG function: function code U; Type III secretory pathway, lipoprotein EscJ

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the yscJ lipoprotein family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRGK_SALTY (P41786)

Other databases:

- EMBL:   U21676
- EMBL:   AE006468
- PIR:   S69786
- RefSeq:   NP_461792.1
- ProteinModelPortal:   P41786
- SMR:   P41786
- PRIDE:   P41786
- GeneID:   1254394
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2871
- HOGENOM:   HBG671305
- OMA:   HISVIAI
- ProtClustDB:   PRK15324
- BioCyc:   STYP99287:STM2871-MONOMER
- GO:   GO:0009405
- InterPro:   IPR003282
- InterPro:   IPR006182
- PRINTS:   PR01338
- TIGRFAMs:   TIGR02544

Pfam domain/function: PF01514 YscJ_FliF

EC number: NA

Molecular weight: Translated: 28211; Mature: 28211

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1fab6604)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRYLYTFLLVMTLAGCKDKDLLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITV
CHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
AEPDFTAAVYWIKTYQLPPRPRVEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTM
ECCCCCEEEEEEEEECCCCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EGVLSARVHISYDIDAGENGRPPKPVHLSALAVYERGSPLAHQISDIKRFLKNSFADVDY
HHHHHEEEEEEEECCCCCCCCCCCCEEEHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
DNISVVLSERSDAQLQAPGTPVKRNSFATSWIVLIILLSVMSAGFGVWYYKNHYARNKKG
CCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCC
ITADDKAKSSNE
CCCCCCCCCCCH
>Mature Secondary Structure
MIRRYLYTFLLVMTLAGCKDKDLLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITV
CHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
AEPDFTAAVYWIKTYQLPPRPRVEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTM
ECCCCCEEEEEEEEECCCCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EGVLSARVHISYDIDAGENGRPPKPVHLSALAVYERGSPLAHQISDIKRFLKNSFADVDY
HHHHHEEEEEEEECCCCCCCCCCCCEEEHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
DNISVVLSERSDAQLQAPGTPVKRNSFATSWIVLIILLSVMSAGFGVWYYKNHYARNKKG
CCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCC
ITADDKAKSSNE
CCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476203; 11677609