Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 194737220

GI number: 194737220

Start: 405202

End: 406659

Strand: Reverse

Name: oprM [H]

Synonym: SeSA_A0403

Alternate gene names: 194737220

Gene position: 406659-405202 (Counterclockwise)

Preceding gene: 194737679

Following gene: 290241785

Centisome position: 8.64

GC content: 59.47

Gene sequence:

>1458_bases
ATGAAGATCATTTTTACAGGCTATCGGCAGACCGCCACGCTGGCGACGCTTGCTTTCGTCACCACGCTTGCTGGCTGTAC
GATGGCGCCGAAACACGAACGCCCCGCATCGCCGACTGCGATGGTCTACCCCTACGCAACGTCAACCGTTTCTGGCGCGC
CGGATGCCGCTGACATTGGCTGGCGTGATTTCTTCCACGATCCGCTTCTACAGGAACTGATCGCGATCGCGTTACGCAAT
AATCGGGATTTACGCAAGGCAGGGCTCAATGTTGAAGCCGCCCGGGCGTTATACCGTATTCAGCGCGCGGAGATGCTGCC
GACGCTCGGCATCGCCACCGCCATGGACGCCGGTCGCACTCCCGCCGATCTCAGCGTCACGGACGAGCCTGAGATTAACC
GACGCTACGAGGCGGCTGGGGCGACGACGGCCTGGGAACTGGATCTCTGGGGGCGAGTGCGAAGCCTTAGCGACCAGGCA
TTAGCCGCCTATATGGCGTTTGATGAGACGTACATTGCGGCGCGAATGAGCCTGGTTTCCGAAGTCGCCAGCGCCTGGCT
GACGCTACGGGCTGACCGGGAGCTGCTGCGCTTAACCGAAGATACGCTGGCCGCGCAAAAAAGTTCATACACATTGACAA
CCCAGCTTGCCCGGACAGGCAACGCCACACAGCTCGATCTGCGTATGGCGGAGATCGCGCTGCGTTCTGCCGAAATCAAT
CGCGCGGCGTATACGCGACAGTTGGCGCGGGATCGTAACGCGCTGGAATTGCTGTTGGGCCAGCCGCTCGCGCCTGAACT
GTCGCGTCGACTAAACGAGGCGGTCACGCTTACGGAAGGCACGATCCCGACCACACTGCCAGGCGGATTACCGTCAGATC
TGCTGGTACGCCGCCCGGATATTCGCGCCGCCGAATACAGGCTGCGCGGCGCAAACGCCCGGATAGGCGCAGCGCGCGCC
GCCTTCTTCCCGACCATCAGCCTGACAGGCGCGGCGGGAACGGCCAGCGCGTCTCTTAGCGGACTCTTTGAACCGGGATC
GGGAAGCTGGCGTTTTCTACCGCAAATCACCTTGCCTCTCTTTCACGGCGGCGCATTACGCGCTGACCTGGACAGGGCGC
ATGTCCAAAAACAGATTGAAATCGCCAGGTATGAAAACGTTATTCAGCAAGCCTTTCGCGACGTGGCGGATGGTCTGGCG
GGACAGCGTACGCTGAATGACCAGGTGCAATCAGAACAACGCGCGGTTGAAGCCAGTCAAATCGCCTATGAGCTGGCCGG
ATTCCGTTTTCAGGAAGGCATCGATGACTACCTTACGCTGCTTGATACCCATCGTATGCTTTATGGCGCGCAACAGCGCC
TGGTACGCACACGTCTCATGCAACAGCTGAATATCATTAATCTGTATAAAGCATTAGGCGGCGGTTGGCGGGAATACAGC
GAGAAAAAGCAAGGTTAG

Upstream 100 bases:

>100_bases
TTGACGCCTGTATTTTACGTGGTGGTTCGCAGGATGGCATTAAAGCGTGAGAACCGCATTGATTCGCATGATCAGCAAGC
ATAATGATGGGAATGGCATA

Downstream 100 bases:

>100_bases
CCCAAATAGCGCGCGTCGCCAGTGCGGCACGCGCGCTAAACAGGCATATATTGCCTGCAAAATAAGGTTAACGAGGGCAA
CGATAAACGTTCCCGCTTAA

Product: outer membrane protein OprM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKIIFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDAGRTPADLSVTDEPEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMAFDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLAPELSRRLNEAVTLTEGTIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGAAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGFRFQEGIDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG

Sequences:

>Translated_485_residues
MKIIFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDAGRTPADLSVTDEPEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMAFDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLAPELSRRLNEAVTLTEGTIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGAAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGFRFQEGIDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG
>Mature_485_residues
MKIIFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDAGRTPADLSVTDEPEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMAFDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLAPELSRRLNEAVTLTEGTIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGAAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGFRFQEGIDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=37.9310344827586, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI2367348, Length=481, Percent_Identity=25.5717255717256, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53429; Mature: 53429

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
WRDFFHDPLLQELIAIALRNNRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDAGRT
HHHHHCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
PADLSVTDEPEINRRYEAAGATTAWELDLWGRVRSLSDQALAAYMAFDETYIAARMSLVS
CCCCCCCCCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEEHHHHHHHHHHHHHHH
RAAYTRQLARDRNALELLLGQPLAPELSRRLNEAVTLTEGTIPTTLPGGLPSDLLVRRPD
HHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHEECCC
IRAAEYRLRGANARIGAARAAFFPTISLTGAAGTASASLSGLFEPGSGSWRFLPQITLPL
CCHHHHEECCCCCCCCHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHH
FHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLAGQRTLNDQVQSEQRAVEASQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAYELAGFRFQEGIDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
HHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKKQG
HHCCC
>Mature Secondary Structure
MKIIFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
WRDFFHDPLLQELIAIALRNNRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDAGRT
HHHHHCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
PADLSVTDEPEINRRYEAAGATTAWELDLWGRVRSLSDQALAAYMAFDETYIAARMSLVS
CCCCCCCCCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEEHHHHHHHHHHHHHHH
RAAYTRQLARDRNALELLLGQPLAPELSRRLNEAVTLTEGTIPTTLPGGLPSDLLVRRPD
HHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHEECCC
IRAAEYRLRGANARIGAARAAFFPTISLTGAAGTASASLSGLFEPGSGSWRFLPQITLPL
CCHHHHEECCCCCCCCHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHH
FHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLAGQRTLNDQVQSEQRAVEASQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAYELAGFRFQEGIDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
HHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKKQG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]