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The map label for this gene is phnV [H]

Identifier: 194737201

GI number: 194737201

Start: 490899

End: 491696

Strand: Reverse

Name: phnV [H]

Synonym: SeSA_A0486

Alternate gene names: 194737201

Gene position: 491696-490899 (Counterclockwise)

Preceding gene: 194734791

Following gene: 194737720

Centisome position: 10.44

GC content: 56.39

Gene sequence:

>798_bases
ATGCTAATTTGGTCACCTAAAGGACGCGCAGCGGCGGGCGTTGTGGCTAGCGTACTGTTTATCGTGTTCTTTTTCCTGCC
GCTGGCGGTGATTCTGATGTCCAGCCTTAGCCAGCAGTGGAACGGTATTCTGCCGAGTGGCTTCACGCTGAACCACTTTG
TTAACGCTCTGCACGGTGCGGCATGGGATGCCCTACTTGCCAGCCTCACGATTGGCTTTTGCGCCAGCCTGTTTGCGCTG
CTTTGCGGCGTTTGGGCGGCGCTGGCGCTGCGTCAATATGGGGTGAAAACGCAGAAATGGTTAAGTATGGTGTTCTATCT
GCCGAGTGCGATTCCGTCAGTGTCCGTGGGCCTGGGCATTCTGGTTGCTTTCAGTCAGGGACCATTGCAAATGAACGGCA
CCCTATGGATTGTGCTGACAGCGCACTTTGTGCTGATTTCCGCCTTTACCTTCAGTAACGTGTCGACCGGACTGGCGCGA
ATTTCCGCCGATATTGAAAACGTCGCGTCGAGTCTGGGGGCATCGCCCTGGTATCGTCTGCGTCACGTGACGTTGCCGTT
ATTGATGCCGTGGATGATGTCAGCGCTGGCGCTGAGTTTATCGCTCTCCATGGGGGAGTTGGGCGCGACGATGATGATTT
ATCCGCCGGGCTGGACGACGCTACCGGTGGCTATCTTTAGCCTGACCGACCGTGGAAATATCGCCGACGGCGCGGCGCTG
ACCATTGTGCTGGTGGCCATTACGCTGCTGCTGATGATGAAGCTGGAGCGCATTGCAAAACGGTTGGGGCACAAATAA

Upstream 100 bases:

>100_bases
CGGTCGCCTGCATGATTGCGTTGATTAACATTGTCTTGTCGCTGGGGCTGTTCTCCCTCTATCGCCTGGCGGCATCACGT
ACCGGCGTAAGGAGCTAGCC

Downstream 100 bases:

>100_bases
AACGGTAGGCCGGGTAGGACATAGCCGCCCCCCGGCAAAGGAATTACGGACGATACACCTTCACATTGGCAAACCCCTGC
TCGCGCAGATAGAGCGCCTG

Product: 2-aminoethylphosphonate ABC transporter membrane component PhnV

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQQWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMMSALALSLSLSMGELGATMMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLGHK

Sequences:

>Translated_265_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQQWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMMSALALSLSLSMGELGATMMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLGHK
>Mature_265_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQQWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMMSALALSLSLSMGELGATMMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLGHK

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=227, Percent_Identity=25.9911894273128, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017661
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28479; Mature: 28479

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQQWNGILPSGFTLNHFVNALHGA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWDALLASLTIGFCASLFALLCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
LVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLARISADIENVASSLGASPWYRL
HEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RHVTLPLLMPWMMSALALSLSLSMGELGATMMIYPPGWTTLPVAIFSLTDRGNIADGAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHH
TIVLVAITLLLMMKLERIAKRLGHK
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQQWNGILPSGFTLNHFVNALHGA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWDALLASLTIGFCASLFALLCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
LVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLARISADIENVASSLGASPWYRL
HEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RHVTLPLLMPWMMSALALSLSLSMGELGATMMIYPPGWTTLPVAIFSLTDRGNIADGAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHH
TIVLVAITLLLMMKLERIAKRLGHK
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA