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The map label for this gene is rsmE [H]

Identifier: 194737195

GI number: 194737195

Start: 3156138

End: 3156917

Strand: Direct

Name: rsmE [H]

Synonym: SeSA_A3268

Alternate gene names: 194737195

Gene position: 3156138-3156917 (Clockwise)

Preceding gene: 194736706

Following gene: 194735153

Centisome position: 67.02

GC content: 54.74

Gene sequence:

>780_bases
ATGCCCTGGATAGCCAAACGCCGGGGCCATGACGCGGATTTTTTTATTATGCGTATTCCCCGCATTTATCACCCTGAATT
GTTGACGTCCGGTACGCAGATTTCGTTATGCGAAGATGCGGCCAACCATATTGGTCGTGTACTGCGCATGGGACCGGGAC
AAGCGTTACAGCTGTTTGACGGCAGTAATCAGGTATTCGATGCTGAAATCATTAGCGCCAGTAAGAAAAGCGTTGAAGTG
CAAGTGATGAAAGGCGAAATCGACGATCGTGAATCGCCGCTGCATATCCATCTGGGCCAGGTGATGTCGCGCGGTGAAAA
AATGGAATTTACTATCCAGAAATCGATCGAACTGGGTGTAAGCCTCATTACGCCACTGTTCTCTGAGCGCTGCGGCGTTA
AACTGGATAATGAACGTCTGAACAAAAAGCGCCAGCAGTGGCAAAAGATCGCCATCGCCGCCTGCGAACAATGCGGGCGT
AACCGGGTGCCGGAAATTCGCCCGCCGATGGCGCTGGAGGCCTGGTGCGCCGAGCAAGATTCCGGGCTGAAGCTCAATCT
TCATCCGCGCGCCCATGCCAGCATCAACACTCTGCCGCTGCCGGTTGAACGCGTCCGACTGCTGATCGGCCCGGAAGGCG
GGCTGTCGGCGGACGAAATCGCGATGACCGCGCGCTATCAGTTTACTGATATTCTGTTAGGACCTCGCGTTCTGCGTACT
GAGACAACCGCGCTCACCGCCATTACTGCGCTACAGGTGCGTTTTGGCGACTTAGGATGA

Upstream 100 bases:

>100_bases
GCGCGTATCGCGAAGGTCCAGGGTAATCATAATCCCTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACT
AGCGGGATTCTTGTTAACCC

Downstream 100 bases:

>100_bases
AGCGTTAACGGAGAAAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCACACATCAACATCAAGAAAGACACCA
GTTTCGCTATGCTGCTGGAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPWIAKRRGHDADFFIMRIPRIYHPELLTSGTQISLCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEIISASKKSVEV
QVMKGEIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDNERLNKKRQQWQKIAIAACEQCGR
NRVPEIRPPMALEAWCAEQDSGLKLNLHPRAHASINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRT
ETTALTAITALQVRFGDLG

Sequences:

>Translated_259_residues
MPWIAKRRGHDADFFIMRIPRIYHPELLTSGTQISLCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEIISASKKSVEV
QVMKGEIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDNERLNKKRQQWQKIAIAACEQCGR
NRVPEIRPPMALEAWCAEQDSGLKLNLHPRAHASINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRT
ETTALTAITALQVRFGDLG
>Mature_258_residues
PWIAKRRGHDADFFIMRIPRIYHPELLTSGTQISLCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEIISASKKSVEVQ
VMKGEIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDNERLNKKRQQWQKIAIAACEQCGRN
RVPEIRPPMALEAWCAEQDSGLKLNLHPRAHASINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTE
TTALTAITALQVRFGDLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=93.0041152263374, Blast_Score=462, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28925; Mature: 28794

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWIAKRRGHDADFFIMRIPRIYHPELLTSGTQISLCEDAANHIGRVLRMGPGQALQLFD
CCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEC
GSNQVFDAEIISASKKSVEVQVMKGEIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGV
CCCCEEEHHHHHCCCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHH
SLITPLFSERCGVKLDNERLNKKRQQWQKIAIAACEQCGRNRVPEIRPPMALEAWCAEQD
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
SGLKLNLHPRAHASINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRT
CCCEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHCCCEEEEE
ETTALTAITALQVRFGDLG
HHHHHHHHHHEEEEECCCC
>Mature Secondary Structure 
PWIAKRRGHDADFFIMRIPRIYHPELLTSGTQISLCEDAANHIGRVLRMGPGQALQLFD
CCCCCCCCCCCCEEEEECCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEC
GSNQVFDAEIISASKKSVEVQVMKGEIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGV
CCCCEEEHHHHHCCCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHH
SLITPLFSERCGVKLDNERLNKKRQQWQKIAIAACEQCGRNRVPEIRPPMALEAWCAEQD
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
SGLKLNLHPRAHASINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRT
CCCEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHCCCEEEEE
ETTALTAITALQVRFGDLG
HHHHHHHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]