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The map label for this gene is hsrA [H]

Identifier: 194737161

GI number: 194737161

Start: 3973538

End: 3974965

Strand: Reverse

Name: hsrA [H]

Synonym: SeSA_A4099

Alternate gene names: 194737161

Gene position: 3974965-3973538 (Counterclockwise)

Preceding gene: 194738253

Following gene: 194737339

Centisome position: 84.41

GC content: 52.03

Gene sequence:

>1428_bases
ATGACCAGCAAAAAAGCGCGCAGTATGGCTGGCCTGCCGTGGATCGCGGCGATGGCCTTCTTTATGCAGGCGCTAGACGC
CACCATTTTGAATACCGCTTTACCGGCTATTGCGCATAGCCTTAACCGTTCACCACTCGCCATGCAGTCCGCTATTATCA
GTTATACCCTGACAGTAGCTATGTTAATTCCGGTAAGCGGCTGGCTGGCTGACCGCTTCGGTACGCGTCGCGTCTTTATG
GTTGCCGTAAGTCTGTTTACGTTAGGCTCGCTGGCCTGCGCGCTCTCCAGTTCACTATCTGAATTGGTTATTTTTCGCGT
CGTACAAGGCGTCGGCGGAGCGATGATGATGCCAGTGGCGCGGCTTGCGTTATTGCGAGCCTATCCGCGTAGTGAGCTGC
TGCCTGTTCTTAACTTTGTCACTATGCCGGGGCTGGTAGGTCCGATTCTGGGGCCGGTATTAGGCGGCGTACTGGTGACC
TGGGCAAGCTGGCACTGGATCTTCCTGATTAATATTCCCATTGGTGTTGCAGGCATTCTGTATGCCCGCAAATATATGCC
CAACTTCACCACGCCGCGTCGCAAGTTTGACATGACCGGCTTCTTTCTTTTTGGGTTAAGTCTGGTTTTATTTTCCAGCG
GAATGGAACTGTTTGGCGAAAAGATTGTGGCGACATGGATCGCATCCGCCATTATTTTTTGCAGTATCGTTCTACTATTG
GCCTATATCCGCCACGCCCGCCGTCATCCGACACCGTTAATATCACTATCGCTGTTTAAGACACGCACATTTTCCGTCGG
TATTGCCGGCAACCTCGCCACGCGTCTGGGGACAGGCTGCGTACCTTTTTTGATGCCGTTAATGCTACAGGTTGGTTTTG
GCTACCCGGCTCTGATCGCCGGCTGCATGATGGCGCCGACAGCTTTGGGTTCTATTATCGCGAAATCGACCGTAACGCAG
GTTTTGCGACGTTTGGGATACCGGAAGACTCTGGTCGGCATTACGGTATTTATCGGTCTGATGATTGCCCAGTTTTCGTT
TCAATCTCCCGCCATGCCGATCTGGATGCTCGTACTGCCGCTATTCATTCTTGGAATGGCGATGTCCACTCAATTTACGG
CAATGAATACAATTACTCTTGCGGATCTGACGGACGATAACGCCAGCAGCGGCAACAGCGTTCTGGCGGTTACGCAGCAA
TTGTCTATCAGTTTAGGCGTGGCGATCAGCGCGGCGGTGTTACGTATTTATGAAGGTTTTGCGGGCACCAGTACCGTGGA
ACAGTTCCACTACACCTTTATCACGATGGGGGCGATCACTATCGTGTCCGCGCTGATGTTTATGCTGCTAAGAGCCAAAG
ACGGCAACAATCTGATTAAAGAACGGCATAAATCTAAACCGACCCACGCACCGTCAAAACCGGAGTAA

Upstream 100 bases:

>100_bases
TTGATGCCATCGCTGATGGCGACGGCGAACGCGCTTTCCAGGCCTGCCAGGCGTTGTTAATAGCGCCCAATGAAAGACCA
GATAATTAACAGGATATTGA

Downstream 100 bases:

>100_bases
GCTGAAGTCGCTGTTGTTGCAAGGCGGGCTGCGCCATCCGATGAATCAACACATCAATAGCCAGCTCGCCCAATTCATCT
TTCGGCTGATGGATAGTCGT

Product: inner membrane transport protein YieO

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTSKKARSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFM
VAVSLFTLGSLACALSSSLSELVIFRVVQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVLVT
WASWHWIFLINIPIGVAGILYARKYMPNFTTPRRKFDMTGFFLFGLSLVLFSSGMELFGEKIVATWIASAIIFCSIVLLL
AYIRHARRHPTPLISLSLFKTRTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPALIAGCMMAPTALGSIIAKSTVTQ
VLRRLGYRKTLVGITVFIGLMIAQFSFQSPAMPIWMLVLPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAISAAVLRIYEGFAGTSTVEQFHYTFITMGAITIVSALMFMLLRAKDGNNLIKERHKSKPTHAPSKPE

Sequences:

>Translated_475_residues
MTSKKARSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFM
VAVSLFTLGSLACALSSSLSELVIFRVVQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVLVT
WASWHWIFLINIPIGVAGILYARKYMPNFTTPRRKFDMTGFFLFGLSLVLFSSGMELFGEKIVATWIASAIIFCSIVLLL
AYIRHARRHPTPLISLSLFKTRTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPALIAGCMMAPTALGSIIAKSTVTQ
VLRRLGYRKTLVGITVFIGLMIAQFSFQSPAMPIWMLVLPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAISAAVLRIYEGFAGTSTVEQFHYTFITMGAITIVSALMFMLLRAKDGNNLIKERHKSKPTHAPSKPE
>Mature_474_residues
TSKKARSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFMV
AVSLFTLGSLACALSSSLSELVIFRVVQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVLVTW
ASWHWIFLINIPIGVAGILYARKYMPNFTTPRRKFDMTGFFLFGLSLVLFSSGMELFGEKIVATWIASAIIFCSIVLLLA
YIRHARRHPTPLISLSLFKTRTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPALIAGCMMAPTALGSIIAKSTVTQV
LRRLGYRKTLVGITVFIGLMIAQFSFQSPAMPIWMLVLPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQL
SISLGVAISAAVLRIYEGFAGTSTVEQFHYTFITMGAITIVSALMFMLLRAKDGNNLIKERHKSKPTHAPSKPE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=475, Percent_Identity=86.1052631578947, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=457, Percent_Identity=45.5142231947484, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789042, Length=407, Percent_Identity=32.6781326781327, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=31.322505800464, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI87081983, Length=306, Percent_Identity=27.4509803921569, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788509, Length=168, Percent_Identity=26.7857142857143, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI87082312, Length=184, Percent_Identity=30.9782608695652, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=470, Percent_Identity=26.8085106382979, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=34.1085271317829, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=395, Percent_Identity=25.3164556962025, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51376; Mature: 51245

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKKARSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRVFMVAVSLFTLGSLACALSSSLSELVIFRVVQGVGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVLVTWASWHWIFLINIPIGVAGIL
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHH
YARKYMPNFTTPRRKFDMTGFFLFGLSLVLFSSGMELFGEKIVATWIASAIIFCSIVLLL
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYIRHARRHPTPLISLSLFKTRTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPALIA
HHHHHHHCCCCCEEEHHHHHHHEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
GCMMAPTALGSIIAKSTVTQVLRRLGYRKTLVGITVFIGLMIAQFSFQSPAMPIWMLVLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAISAAVLRIYEGF
HHHHHHHHHCHHHHHEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
AGTSTVEQFHYTFITMGAITIVSALMFMLLRAKDGNNLIKERHKSKPTHAPSKPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSKKARSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRVFMVAVSLFTLGSLACALSSSLSELVIFRVVQGVGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVLVTWASWHWIFLINIPIGVAGIL
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHH
YARKYMPNFTTPRRKFDMTGFFLFGLSLVLFSSGMELFGEKIVATWIASAIIFCSIVLLL
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYIRHARRHPTPLISLSLFKTRTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPALIA
HHHHHHHCCCCCEEEHHHHHHHEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
GCMMAPTALGSIIAKSTVTQVLRRLGYRKTLVGITVFIGLMIAQFSFQSPAMPIWMLVLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAISAAVLRIYEGF
HHHHHHHHHCHHHHHEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
AGTSTVEQFHYTFITMGAITIVSALMFMLLRAKDGNNLIKERHKSKPTHAPSKPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]