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The map label for this gene is miaA [H]

Identifier: 194737154

GI number: 194737154

Start: 4502825

End: 4503775

Strand: Direct

Name: miaA [H]

Synonym: SeSA_A4628

Alternate gene names: 194737154

Gene position: 4502825-4503775 (Clockwise)

Preceding gene: 194734797

Following gene: 194736692

Centisome position: 95.62

GC content: 54.26

Gene sequence:

>951_bases
ATGAATGATGTAAGCAAGGCGAGCCTGCCTAAGGCGATATTTTTGATGGGGCCGACGGCCTCCGGCAAAACGGCGCTGGC
GATAGAACTGCGTAAAGTTTTGCCAGTAGAGTTGATAAGCGTTGATTCCGCCCTCATTTATCGGGGAATGGATATTGGCA
CGGCTAAGCCGAATGCCGATGAGCTGAAAGCGGCGCCGCATCGACTGTTAGATATACGCGACCCGTCGCAGGCGTATTCG
GCGGCGGATTTTCGCCGCGACGCGTTAGCGCAAATGGCGGAGATCACCGCTGCGGGGCGTATCCCGCTGTTAGTGGGCGG
TACCATGCTGTATTTTAAGGCATTGCTGGAAGGCTTATCGCCGCTGCCTTCAGCAGATCCGGAAGTCAGATCCCGGATAG
AGCAGCAAGCAGCGGAGCTTGGATGGGAGGCGTTGCATCAGCAACTTCAGGAGATAGATCCGGTTGCCGCCGCGCGAATT
CATCCAAATGATCCGCAAAGACTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAGCTAACGCA
AACGTCAGGAGACGCGCTACCGTACCAGGTGCATCAGTTCGCTATCGCCCCGGCGAGCCGTGAACTGCTCCATCAGCGCA
TTGAACTGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCAT
ACGGACTTGCCTTCCATCCGTTGTGTGGGATACCGCCAGATGTGGTCATACATTGAGGGCGAAATCTCATATGATGAAAT
GGTTTATAGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGCCAGATGACCTGGTTGCGCGGTTGGGAAGGGGTGCGCT
GGCTTGACAGTGAGAATCCCGATCGGGCGCGAAAAGAAGTATTACAGGTTGTTGGTGCTATCGCAGACTGA

Upstream 100 bases:

>100_bases
CTGGCGGATGTTGAGCGGCTATGTCCGCAGCTGGTAAAAGCGCCGCCGGGTGGCCTGTTACAACCTGTTGATTTACATTC
GGCGATGAACGCCCTGAAGC

Downstream 100 bases:

>100_bases
ATGTGTACAATTGAGACGTATCGTGCGCAATTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGA
GAATGGCTAAGGGGCAATCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNDVSKASLPKAIFLMGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNADELKAAPHRLLDIRDPSQAYS
AADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRSRIEQQAAELGWEALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIELRFHQMLASGFEAEVRALFARGDLH
TDLPSIRCVGYRQMWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENPDRARKEVLQVVGAIAD

Sequences:

>Translated_316_residues
MNDVSKASLPKAIFLMGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNADELKAAPHRLLDIRDPSQAYS
AADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRSRIEQQAAELGWEALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIELRFHQMLASGFEAEVRALFARGDLH
TDLPSIRCVGYRQMWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENPDRARKEVLQVVGAIAD
>Mature_316_residues
MNDVSKASLPKAIFLMGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNADELKAAPHRLLDIRDPSQAYS
AADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRSRIEQQAAELGWEALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIELRFHQMLASGFEAEVRALFARGDLH
TDLPSIRCVGYRQMWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENPDRARKEVLQVVGAIAD

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=27.9365079365079, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1790613, Length=315, Percent_Identity=93.3333333333333, Blast_Score=607, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI25144712, Length=298, Percent_Identity=26.510067114094, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=25.9515570934256, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24649830, Length=277, Percent_Identity=29.9638989169675, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35171; Mature: 35171

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVSKASLPKAIFLMGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNAD
CCCCCHHCCCCEEEEECCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
ELKAAPHRLLDIRDPSQAYSAADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLS
HHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECHHHHHHHHHHHCCC
PLPSADPEVRSRIEQQAAELGWEALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHCCCC
TLTELTQTSGDALPYQVHQFAIAPASRELLHQRIELRFHQMLASGFEAEVRALFARGDLH
HHHHHHHCCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
TDLPSIRCVGYRQMWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCH
DRARKEVLQVVGAIAD
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDVSKASLPKAIFLMGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNAD
CCCCCHHCCCCEEEEECCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
ELKAAPHRLLDIRDPSQAYSAADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLS
HHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECHHHHHHHHHHHCCC
PLPSADPEVRSRIEQQAAELGWEALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHHCCCC
TLTELTQTSGDALPYQVHQFAIAPASRELLHQRIELRFHQMLASGFEAEVRALFARGDLH
HHHHHHHCCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
TDLPSIRCVGYRQMWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCH
DRARKEVLQVVGAIAD
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA