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The map label for this gene is ushA [H]

Identifier: 194736935

GI number: 194736935

Start: 561873

End: 563525

Strand: Direct

Name: ushA [H]

Synonym: SeSA_A0557

Alternate gene names: 194736935

Gene position: 561873-563525 (Clockwise)

Preceding gene: 194735078

Following gene: 194737943

Centisome position: 11.93

GC content: 51.97

Gene sequence:

>1653_bases
ATGAAATTTTTGAAACGGGGTGTGGCGCTGGCGTTACTGGCGGCGTTCGCGCTGACGACTCAGCCTGCACAGGCTTACGA
AAAAGATAAAACCTATAAAATTACTATCCTGCATACCAACGATCACCACGGTCACTTCTGGCGCAGCGAATATGGCGAAT
ATGGTCTGGCGGCGCAAAAAACGCTGGTGGACAGTATCCGTAAAGAGGTGGCGCAAGAGGGGGGAAGCGTCCTGTTGTTA
TCCGGCGGCGACATTAATACCGGGGTGCCGGAATCCGATCTCCAGGATGCGGAGCCCGATTTCCGCGGGATGAATCTGAT
TGGCTACGACGCTATGGCCGTCGGTAATCATGAGTTTGATAACCCGCTCACCGTATTGCGCCAGCAGGAAAAGTGGGCGA
AGTTTCCCTTTCTTTCCGCCAATATTTATCAAAAAAGTACCGGCGAGCGTCTGTTTAAGCCGTGGGCTATTTTTACACGC
CAGGATATAAAAATCGCGGTAATTGGCTTAACCACCGATGACACGGCGAAAATAGGCAACCCGGAATATTTCACCGATAT
TGAGTTTCGTAAACCTGCTGAAGAAGCAAACGTGGTGATTCAGGAACTTAATATGAATGAAAAACCGGACGTGATTATCG
CGACCACGCATATGGGACATTATGACAACGGCGATCACGGTTCGAATGCGCCGGGCGACGTTGAGATGGCGCGTAGCCTG
CCTGCCGGTTCGCTGGCGATGATTGTGGGCGGTCACTCACAAGACCCGGTATGCATGACGTCGGAAAATAAAAAACAGGT
GAATTACGTACCGGGAACGCCCTGCGCGCCGGATAAGCAAAATGGCATCTGGATCGTGCAGGCGCATGAGTGGGGTAAAT
ATGTGGGCCGCGCGGATTTCGAATTCCGTAACGGCGAGATGAAAATGGTTAACTACCAGCTTATTCCGGTAAATCTCAAG
AAAAAAGTGACCTGGGATAACGGGAAAAGCGAGCGTGTACTTTACACGCCGGAAATCGCAGAAAATCCGCAAATGCTCTC
GTTATTAACACCGTTCCAGAATAAAGGTAAAGCGCAACTGGAGGTGAAAATTGGTAGCGTGAATGGCCTTCTTGAAGGCG
ATCGCAGTAAGGTCAGATTTGTCCAGACCAATATGGGACGGGTGATTCTGGCTGCGCAGATCGCGCGCACCGGCGCCGAT
TTTGGCGTGATGAGCGGCGGCGGTATTCGCGACTCGATTGAGGCGGGAGATATTACCTATAAAAGCGTGCTCAAGGTACA
GCCGTTCGGCAACATTGTGGTGTATGCCGATATGAGTGGCAAAGAGGTGGTTGATTATCTCACCGCCGTAGCGCAGATGA
AACCGGACTCCGGCGCCTATCCACAGTTCGCCAATGTGAGCTTTGTCGCCAAAGAGGGCAAGCTCACCGATCTGAAAATC
AAAGGCGAGCCTGTTGATCCGGCCAAAACCTACCGCATGGCGACGCTGAGTTTCAACGCCACGGGCGGCGATGGTTATCC
GCGCATCGATAACAAACCGGGATACGTGAATACCGGGTTTATTGACGCGGAAGTGCTGAAAGAGTTTATTCAGCAGAATT
CACCGCTGGATGCGGCGGCGTTTACGCCAAAAGGCGAGGTGAGCTGGCTGTAG

Upstream 100 bases:

>100_bases
TGAAATGTGTTTGAAGGAAGTCTCAATTCTGAAAACATATTTGTCTATTATTGCAAGGAAAGGTAATTTCTGCGGTTGAT
ATTGAGTCAGGGAGAGAAAG

Downstream 100 bases:

>100_bases
CGGTACGGATAGCCATTTCTGCCTGATGGCGCGCAGCCTTAAGGCCTACGGATTATGTCTGCTGTAGGCCGGATAAGGCG
TTTGCGCCGCTATCCGGCAA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLSANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEANVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMTSENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPKGEVSWL

Sequences:

>Translated_550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLSANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEANVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMTSENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPKGEVSWL
>Mature_550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLSANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEANVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMTSENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPKGEVSWL

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=586, Percent_Identity=25.5972696245734, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=87.7959927140255, Blast_Score=1031, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=560, Percent_Identity=22.3214285714286, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922446, Length=583, Percent_Identity=25.7289879931389, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24654424, Length=583, Percent_Identity=25.7289879931389, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28573524, Length=556, Percent_Identity=24.2805755395683, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922444, Length=571, Percent_Identity=22.4168126094571, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641187, Length=546, Percent_Identity=21.6117216117216, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221329836, Length=575, Percent_Identity=21.7391304347826, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60545; Mature: 60545

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHH
TLVDSIRKEVAQEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLTVLRQQEKWAKFPFLSANIYQKSTGERLFKPWAIFTRQDIKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEECCEECCCCCCHHHCCEEEEEECCEEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEANVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
CCEEEEECCCCCCHHHCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMTSENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKMVNYQLIPVNLKKKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEECEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEE
EVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGADFGVMSGGGIRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCEEE
KSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKEGKLTDLKI
EHEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCCEE
FTPKGEVSWL
CCCCCCCCCC
>Mature Secondary Structure
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHH
TLVDSIRKEVAQEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLTVLRQQEKWAKFPFLSANIYQKSTGERLFKPWAIFTRQDIKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEECCEECCCCCCHHHCCEEEEEECCEEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEANVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
CCEEEEECCCCCCHHHCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMTSENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKMVNYQLIPVNLKKKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEECEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEE
EVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGADFGVMSGGGIRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCEEE
KSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQFANVSFVAKEGKLTDLKI
EHEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCCEE
FTPKGEVSWL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11429465 [H]