Click here to switch to the map view.

The map label for this gene is prpD [H]

Identifier: 194736852

GI number: 194736852

Start: 432968

End: 434419

Strand: Direct

Name: prpD [H]

Synonym: SeSA_A0422

Alternate gene names: 194736852

Gene position: 432968-434419 (Clockwise)

Preceding gene: 194737491

Following gene: 194736206

Centisome position: 9.19

GC content: 56.54

Gene sequence:

>1452_bases
ATGTCTACCCAAGAACTGAACATCCGCCCGGAATTTGACCGCGAAATCGTCGATATCGTGGATTACGTGATGAACTACGA
CATCACCTCAAAGGTGGCGTACGACACCGCGCATTATTGCCTGCTCGACACGCTCGGCTGTGGTCTGGAAGCGCTGGAAT
ACCCGGCCTGTAAAAAATTGCTTGGGCCGATCGTACCAGGCACGGTGGTGCCCAACGGCGCACGCGTGCCGGGCACCCAG
TTCCAGCTCGATCCGGTACAGGCAGCTTTTAACATTGGCGCGATGATCCGCTGGCTCGATTTTAACGATACCTGGCTTGC
CGCCGAGTGGGGCCATCCTTCTGATAACCTCGGCGGTATTCTGGCGATTGCGGATTGGTTGTCACGTAACGCCGTCGCCG
CCGGCAAAGCGCCGCTGACCATGAAACAGGTATTGAGTGGGATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTG
GAAAACGCCTTCAACCGTGTCGGACTTGACCATGTGTTGCTGGTGAAAGTGGCCTCGACTGCGGTGGTCGCTGAAATGCT
GGGGCTGACGCGCGATGAGATCCTCAACGCGGTATCGCTGGCGTGGGTGGATGGGCAGTCGTTGCGCACTTATCGTCATG
CGCCGAATACCGGTACGCGCAAATCCTGGGCGGCGGGCGATGCGACTTCGCGCGCGGTACGTCTGGCGCTGATGGCGAAA
ACCGGCGAGATGGGCTATCCCTCGGCGCTCACCGCCAAAACCTGGGGCTTCTACGACGTTTCATTCAAAGGTGAAACGTT
CCGTTTCCAGCGTCCTTATGGCTCCTACGTGATGGAAAACGTGCTATTCAAAATTTCTTTCCCGGCAGAATTCCACTCGC
AAACCGCCGTCGAAGCGGCGATGACGCTGTATGAGCAGATGCAGGCCGCGGGTAAAACGGCAGCGGATATCGAGAAAGTG
ACCATCCGCACCCACGAAGCCTGTCTCCGCATTATCGATAAAAAAGGCCCGCTCAATAACCCGGCGGACCGCGATCACTG
TATCCAGTATATGGTCGCCGTGCCGTTGCTGTTCGGACGGTTAACCGCGGCGGATTATGAAGACGAGGTGGCGCAGGACA
AGCGTATTGACGCCCTGCGCGAGAAAATCGTGTGTTATGAGGATCCGGCTTTTACCGCCGACTATCACGACCCGGAAAAA
CGTGCTATCGGCAATGCGATCACCGTGGAGTTTACTGATGGCTCACGCTTTGGCGAGGTTGTCGTGGAGTATCCGATTGG
TCATGCGCGTCGCCGCGCCGACGGTATTCCGAAGCTTATCGAAAAATTTAAAATAAACCTGGCGCGTCAGTTCCCGACTC
GCCAGCAGCAACGCATTCTGGATGTCTCCCTGGACAGAGCCCGCCTGGAGCAGATGCCGGTTAACGAATACCTCGATTTA
TATGTCATCTGA

Upstream 100 bases:

>100_bases
CTCTGCCAACTATACCGGGCCGGAAGATCGTCCGTTTGTCTCGATAGACGATCGTTGCTAACTCACTCTTATTAAAAATA
AAACGCAGGAAACGTACCCT

Downstream 100 bases:

>100_bases
GCCAGCCAGCAGTAAGGCGTAAGTTCAACAGGAGAGCGTGATGTCTTTTAGCGAATTTTATCAGCGTTCCATTAACGAAC
CGGAGGCGTTCTGGGCCGAG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSTQELNIRPEFDREIVDIVDYVMNYDITSKVAYDTAHYCLLDTLGCGLEALEYPACKKLLGPIVPGTVVPNGARVPGTQ
FQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAIADWLSRNAVAAGKAPLTMKQVLSGMIKAHEIQGCIAL
ENAFNRVGLDHVLLVKVASTAVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
TGEMGYPSALTAKTWGFYDVSFKGETFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKV
TIRTHEACLRIIDKKGPLNNPADRDHCIQYMVAVPLLFGRLTAADYEDEVAQDKRIDALREKIVCYEDPAFTADYHDPEK
RAIGNAITVEFTDGSRFGEVVVEYPIGHARRRADGIPKLIEKFKINLARQFPTRQQQRILDVSLDRARLEQMPVNEYLDL
YVI

Sequences:

>Translated_483_residues
MSTQELNIRPEFDREIVDIVDYVMNYDITSKVAYDTAHYCLLDTLGCGLEALEYPACKKLLGPIVPGTVVPNGARVPGTQ
FQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAIADWLSRNAVAAGKAPLTMKQVLSGMIKAHEIQGCIAL
ENAFNRVGLDHVLLVKVASTAVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
TGEMGYPSALTAKTWGFYDVSFKGETFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKV
TIRTHEACLRIIDKKGPLNNPADRDHCIQYMVAVPLLFGRLTAADYEDEVAQDKRIDALREKIVCYEDPAFTADYHDPEK
RAIGNAITVEFTDGSRFGEVVVEYPIGHARRRADGIPKLIEKFKINLARQFPTRQQQRILDVSLDRARLEQMPVNEYLDL
YVI
>Mature_482_residues
STQELNIRPEFDREIVDIVDYVMNYDITSKVAYDTAHYCLLDTLGCGLEALEYPACKKLLGPIVPGTVVPNGARVPGTQF
QLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILAIADWLSRNAVAAGKAPLTMKQVLSGMIKAHEIQGCIALE
NAFNRVGLDHVLLVKVASTAVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAKT
GEMGYPSALTAKTWGFYDVSFKGETFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVT
IRTHEACLRIIDKKGPLNNPADRDHCIQYMVAVPLLFGRLTAADYEDEVAQDKRIDALREKIVCYEDPAFTADYHDPEKR
AIGNAITVEFTDGSRFGEVVVEYPIGHARRRADGIPKLIEKFKINLARQFPTRQQQRILDVSLDRARLEQMPVNEYLDLY
VI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=483, Percent_Identity=94.2028985507246, Blast_Score=957, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=485, Percent_Identity=57.9381443298969, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53799; Mature: 53668

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQELNIRPEFDREIVDIVDYVMNYDITSKVAYDTAHYCLLDTLGCGLEALEYPACKKL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPIVPGTVVPNGARVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
HCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCHH
LAIADWLSRNAVAAGKAPLTMKQVLSGMIKAHEIQGCIALENAFNRVGLDHVLLVKVAST
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
AVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
HHHHHHHCCCHHHHHHHHHEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEE
TGEMGYPSALTAKTWGFYDVSFKGETFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAA
CCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHEEEECCHHHHHHHHHHHH
MTLYEQMQAAGKTAADIEKVTIRTHEACLRIIDKKGPLNNPADRDHCIQYMVAVPLLFGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDEVAQDKRIDALREKIVCYEDPAFTADYHDPEKRAIGNAITVEFTDGSRFGEV
HHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCE
VVEYPIGHARRRADGIPKLIEKFKINLARQFPTRQQQRILDVSLDRARLEQMPVNEYLDL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHCEE
YVI
EEC
>Mature Secondary Structure 
STQELNIRPEFDREIVDIVDYVMNYDITSKVAYDTAHYCLLDTLGCGLEALEYPACKKL
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPIVPGTVVPNGARVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
HCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCHH
LAIADWLSRNAVAAGKAPLTMKQVLSGMIKAHEIQGCIALENAFNRVGLDHVLLVKVAST
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
AVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
HHHHHHHCCCHHHHHHHHHEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEE
TGEMGYPSALTAKTWGFYDVSFKGETFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAA
CCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHEEEECCHHHHHHHHHHHH
MTLYEQMQAAGKTAADIEKVTIRTHEACLRIIDKKGPLNNPADRDHCIQYMVAVPLLFGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDEVAQDKRIDALREKIVCYEDPAFTADYHDPEKRAIGNAITVEFTDGSRFGEV
HHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCE
VVEYPIGHARRRADGIPKLIEKFKINLARQFPTRQQQRILDVSLDRARLEQMPVNEYLDL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHCEE
YVI
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]