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The map label for this gene is trmJ [H]

Identifier: 194736797

GI number: 194736797

Start: 2684691

End: 2685422

Strand: Reverse

Name: trmJ [H]

Synonym: SeSA_A2786

Alternate gene names: 194736797

Gene position: 2685422-2684691 (Counterclockwise)

Preceding gene: 194735065

Following gene: 194737710

Centisome position: 57.03

GC content: 56.97

Gene sequence:

>732_bases
ATGCTGCAAAACATTCGAATTGTGCTGGTGGAAACCTCTCACACCGGCAATATGGGCTCTGTGGCTCGCGCTATGAAAAC
CATGGGCTTAACTAACCTGTGGCTGGTAAACCCGCTGGTGAAACCGGACTCCCAGGCCATCGCCTTGGCGGCAGGCGCCA
GCGATGTGATCGGTAATGCGCAGATCGTCGATACGCTGGATGAAGCGTTGGCTGGCTGTAGCCTGGTGGTCGGCACCAGC
GCCCGTTCCCGCACTCTGCCGTGGCCGATGCTTGATCCGCGCGAGTGCGGCTTAAAAAGCGTGGCGGAAGCGGCAAATAC
GCCGGTTGCGCTGGTTTTTGGTCGTGAGCGTGTCGGGCTGACTAACGATGAGTTGCAAAAATGCCATTATCACGTCGCGA
TTGCCGCTAACCCGGAGTACAGCTCGTTGAATCTGGCGATGGCGGTACAGGTCATCGCGTATGAAGTCCGTATGGCCTGG
CTGGCGACGCAGGAAAACGGCGACGCGGCAGATCATGAAGAGACGCCGTATCCGCTGGTGGACGATCTTGAACGTTTCTA
CGGCCATCTGGAGCAGACGTTGCTCTCCACTGGCTTTATTCGCGAAAGCCATCCGGGGCAGGTGATGAATAAATTGCGCC
GTCTGTTTACCCGCGCCCGCCCGGAAAGCCAGGAGTTGAATATTCTGCGTGGGATTCTGGCGTCTATTGAGCAGCAGAAT
AAAGGCAAGTAA

Upstream 100 bases:

>100_bases
GTTAAAGAACGGGAAAACGGCGCAGAGTATAGCAGCGAGATCGGAATCTGTCTTGGTTTTATGGTAAGATGTCAGAATAA
TCTTTAGCTGAAGAAAGACA

Downstream 100 bases:

>100_bases
CCGCGTCAATACCGCACGGAACGCTAAATACCTGACTAATTTACTCAAGTAAATAGTTGACCAAATTACTCGGGAATGTC
AGACTTGTCCCTGCTATGCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGDAADHEETPYPLVDDLERFYGHLEQTLLSTGFIRESHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGK

Sequences:

>Translated_243_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGDAADHEETPYPLVDDLERFYGHLEQTLLSTGFIRESHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGK
>Mature_243_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGDAADHEETPYPLVDDLERFYGHLEQTLLSTGFIRESHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=96.6942148760331, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=36.6013071895425, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26587; Mature: 26587

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHHCCH
QIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGDAADHEETPYPLV
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLLSTGFIRESHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGK
CCC
>Mature Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHHCCH
QIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGDAADHEETPYPLV
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLLSTGFIRESHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA