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The map label for this gene is iadA [H]

Identifier: 194736715

GI number: 194736715

Start: 4629194

End: 4630366

Strand: Reverse

Name: iadA [H]

Synonym: SeSA_A4768

Alternate gene names: 194736715

Gene position: 4630366-4629194 (Counterclockwise)

Preceding gene: 194737185

Following gene: 194734115

Centisome position: 98.33

GC content: 59.85

Gene sequence:

>1173_bases
ATGCCTGATTTATCCGCCGCGGAGTTTACCTTGTTACAGGGCGCGCACCTGTTTGCGCCAGAAGACCGGGGCATCTGCGA
TGTTCTGCTCGCGAATGGCAAAATTATTGCCGTCGGCGCTGACATCCCCGGCGATATCGTACCGGACTGTGCGGTGATTA
ATCTTAGCGGTCGTATGCTGTGCCCGGGCTTTATCGACCAACACGTTCATCTGATCGGCGGCGGCGGCGAAGCGGGGCCG
ACGACGCGGACGCCGGAAGTCTCCCTGAGCCGCCTGACGGAAGCCGGGATAACAACGGTCGTCGGCCTGCTTGGCACCGA
CTCCGTCAGCCGTCATCCCGCGTCGCTGTTAGCCAAAACGCGAGCGCTAAATGAGGAGGGGATCACCGCCTGGATGCTCA
CGGGCGCTTATCACGTTCCGTCGCCGACCATTACTGGCTCGGTAGAAAAAGACGTCGCGCTGATCGATCGCGTCATTGGC
GTGAAATGCGCAGTCTCCGACCACCGTTCCGCCGCGCCCGGCGGGAATCAACTCGCCAGCATGGCCGCCGAATCACGCGT
TGGCGGCCTGCTGGGCGGTAAACCCGGTGTCAGCGTTTTTCATATGGGCAGCAGCAAAAAAGGGTTACAGCCGCTGTATG
ACATCCTGGAAAACAGCGACGTGCCGATCGGTAAACTGCTGCCTACCCACGTCAACCGTAGCGAATCTCTTTTCGAGCAG
GCGCTGGCGTTTGCGCTTAAAGGCGGCGTAATCGACATTACCACCAGTATCCCGGATCCCGTCGCGCCGGCGGAGGGCAT
TGCCAGAGCGGTAAAAGCCGGCGTCCCGCTTTCCCGGGTGACGCTCAGTTCAGACGGTAACGGCAGCCAGCCGCTATTTG
ACGCTGCCGGAAATCTGACGGGTATCGGCGTCGCGGGCTTCGAAAGTTTGCTGGAGACGTTGCAGACGCTGGTTAACCAC
TACGGATTCAGCCTGACCGATGCTCTGCGCCCGCTGACGACCAGCGTAGCCGCCTTCCTCAGTCTGGACAGCAAAGGCGA
AATCCGTCCCGGTAACGATGCGGATCTGCTTGTCTTTAGCGCAGATTTACGCATTGAGCAGGTGTATGCGCGCGGCAAAC
GCATGGTCAATGAGGGGAAAGCCTGCGTAAAAGGTACCTTTGAACCGGCGTAA

Upstream 100 bases:

>100_bases
AGTCAACGCCAAATATTACCCGCATATTATCGCAGTCTGCGCCATCAATGCGCTGCTGTCGATCTGGGTCATGCAGCTTA
TTGTTTAATAAGGAGTTATC

Downstream 100 bases:

>100_bases
TTCCCGCCAGTTTCAGGCTGCCCCATCACGGCATTCTGAAAGAGGACGGGATAAGCGGTTGTCAGCCGCCCGAATGGACG
TAGAATGCCCTTTCGGGCGG

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPDLSAAEFTLLQGAHLFAPEDRGICDVLLANGKIIAVGADIPGDIVPDCAVINLSGRMLCPGFIDQHVHLIGGGGEAGP
TTRTPEVSLSRLTEAGITTVVGLLGTDSVSRHPASLLAKTRALNEEGITAWMLTGAYHVPSPTITGSVEKDVALIDRVIG
VKCAVSDHRSAAPGGNQLASMAAESRVGGLLGGKPGVSVFHMGSSKKGLQPLYDILENSDVPIGKLLPTHVNRSESLFEQ
ALAFALKGGVIDITTSIPDPVAPAEGIARAVKAGVPLSRVTLSSDGNGSQPLFDAAGNLTGIGVAGFESLLETLQTLVNH
YGFSLTDALRPLTTSVAAFLSLDSKGEIRPGNDADLLVFSADLRIEQVYARGKRMVNEGKACVKGTFEPA

Sequences:

>Translated_390_residues
MPDLSAAEFTLLQGAHLFAPEDRGICDVLLANGKIIAVGADIPGDIVPDCAVINLSGRMLCPGFIDQHVHLIGGGGEAGP
TTRTPEVSLSRLTEAGITTVVGLLGTDSVSRHPASLLAKTRALNEEGITAWMLTGAYHVPSPTITGSVEKDVALIDRVIG
VKCAVSDHRSAAPGGNQLASMAAESRVGGLLGGKPGVSVFHMGSSKKGLQPLYDILENSDVPIGKLLPTHVNRSESLFEQ
ALAFALKGGVIDITTSIPDPVAPAEGIARAVKAGVPLSRVTLSSDGNGSQPLFDAAGNLTGIGVAGFESLLETLQTLVNH
YGFSLTDALRPLTTSVAAFLSLDSKGEIRPGNDADLLVFSADLRIEQVYARGKRMVNEGKACVKGTFEPA
>Mature_389_residues
PDLSAAEFTLLQGAHLFAPEDRGICDVLLANGKIIAVGADIPGDIVPDCAVINLSGRMLCPGFIDQHVHLIGGGGEAGPT
TRTPEVSLSRLTEAGITTVVGLLGTDSVSRHPASLLAKTRALNEEGITAWMLTGAYHVPSPTITGSVEKDVALIDRVIGV
KCAVSDHRSAAPGGNQLASMAAESRVGGLLGGKPGVSVFHMGSSKKGLQPLYDILENSDVPIGKLLPTHVNRSESLFEQA
LAFALKGGVIDITTSIPDPVAPAEGIARAVKAGVPLSRVTLSSDGNGSQPLFDAAGNLTGIGVAGFESLLETLQTLVNHY
GFSLTDALRPLTTSVAAFLSLDSKGEIRPGNDADLLVFSADLRIEQVYARGKRMVNEGKACVKGTFEPA

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=390, Percent_Identity=80.7692307692308, Blast_Score=645, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 40384; Mature: 40252

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLSAAEFTLLQGAHLFAPEDRGICDVLLANGKIIAVGADIPGDIVPDCAVINLSGRML
CCCCCCHHHHHHCCCEEECCCCCCCEEEEEECCEEEEECCCCCCCCCCCEEEEECCCCEE
CPGFIDQHVHLIGGGGEAGPTTRTPEVSLSRLTEAGITTVVGLLGTDSVSRHPASLLAKT
CCCHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
RALNEEGITAWMLTGAYHVPSPTITGSVEKDVALIDRVIGVKCAVSDHRSAAPGGNQLAS
HHCCCCCCEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHH
MAAESRVGGLLGGKPGVSVFHMGSSKKGLQPLYDILENSDVPIGKLLPTHVNRSESLFEQ
HHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHH
ALAFALKGGVIDITTSIPDPVAPAEGIARAVKAGVPLSRVTLSSDGNGSQPLFDAAGNLT
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCEE
GIGVAGFESLLETLQTLVNHYGFSLTDALRPLTTSVAAFLSLDSKGEIRPGNDADLLVFS
EECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
ADLRIEQVYARGKRMVNEGKACVKGTFEPA
CCCHHHHHHHHHHHHHHCCCHHCCCCCCCC
>Mature Secondary Structure 
PDLSAAEFTLLQGAHLFAPEDRGICDVLLANGKIIAVGADIPGDIVPDCAVINLSGRML
CCCCCHHHHHHCCCEEECCCCCCCEEEEEECCEEEEECCCCCCCCCCCEEEEECCCCEE
CPGFIDQHVHLIGGGGEAGPTTRTPEVSLSRLTEAGITTVVGLLGTDSVSRHPASLLAKT
CCCHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
RALNEEGITAWMLTGAYHVPSPTITGSVEKDVALIDRVIGVKCAVSDHRSAAPGGNQLAS
HHCCCCCCEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHH
MAAESRVGGLLGGKPGVSVFHMGSSKKGLQPLYDILENSDVPIGKLLPTHVNRSESLFEQ
HHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHH
ALAFALKGGVIDITTSIPDPVAPAEGIARAVKAGVPLSRVTLSSDGNGSQPLFDAAGNLT
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCEE
GIGVAGFESLLETLQTLVNHYGFSLTDALRPLTTSVAAFLSLDSKGEIRPGNDADLLVFS
EECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
ADLRIEQVYARGKRMVNEGKACVKGTFEPA
CCCHHHHHHHHHHHHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]