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The map label for this gene is endA [H]

Identifier: 194736706

GI number: 194736706

Start: 3155402

End: 3156109

Strand: Direct

Name: endA [H]

Synonym: SeSA_A3267

Alternate gene names: 194736706

Gene position: 3155402-3156109 (Clockwise)

Preceding gene: 194738157

Following gene: 194737195

Centisome position: 67.01

GC content: 56.07

Gene sequence:

>708_bases
ATGTACCGTAATTTTTCTTTTGCCGCTGCGTTGCTGGCCGCAGCGTTTTCAGGCCAGGCCCTGGCCGATGGCATTAACAA
TTTTTCTCAGGCCAAAGCGGCGAGCGTCAAAGTCAATGCCGACGCGCCCGGCAGCTTTTACTGCGGGTGCCAAATCCGCT
GGCAGGGTAAAAAAGGCGTCGTAGACCTGGAGTCCTGCGGCTATAAGGTGCGTAAAAACGAGAATCGCGCCAGACGCATT
GAGTGGGAGCACGTTGTCCCCGCCTGGCAATTCGGTCATCAGCGCCAGTGCTGGCAGGACGGCGGGCGAAAAAACTGCGC
TAAAGACCCGGTCTACCGCAAAATGGAAAGCGATATGCATAACCTGCAACCCGCCATTGGCGAAGTGAATGGCGATCGCG
GCAACTTTATGTATAGCCAGTGGAACGGCGGCGAAGGTCAGTACGGGCAATGCGCCATGAAAGTGGATTTTAAAGCGAAA
ATCGCCGAGCCGCCCGCCCGCGCCCGTGGCGCAATCGCCCGCACTTATTTTTATATGCGCGACCAATACCAACTGAAACT
TTCCCGCCAACAAACGCAGCTGTTTAACGTCTGGGATAAGCAGTACCCCGTTACTGCCTGGGAGTGCGAGCGCGATGCGC
GTATCGCGAAGGTCCAGGGTAATCATAATCCCTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAA

Upstream 100 bases:

>100_bases
GGGTAGTTCCCGAAACGTCCGGGAACTTTCCTGAGCGGACTGATTGCATACAGACACAACTTTCGTTACGTTGCGGGCTC
GTTTTGCTATGGAGTGTGCA

Downstream 100 bases:

>100_bases
CCTACACTAGCGGGATTCTTGTTAACCCATGCCCTGGATAGCCAAACGCCGGGGCCATGACGCGGATTTTTTTATTATGC
GTATTCCCCGCATTTATCAC

Product: endonuclease I

Products: NA

Alternate protein names: Endonuclease I; Endo I [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MYRNFSFAAALLAAAFSGQALADGINNFSQAKAASVKVNADAPGSFYCGCQIRWQGKKGVVDLESCGYKVRKNENRARRI
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPAIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKAK
IAEPPARARGAIARTYFYMRDQYQLKLSRQQTQLFNVWDKQYPVTAWECERDARIAKVQGNHNPYVQRACQARKS

Sequences:

>Translated_235_residues
MYRNFSFAAALLAAAFSGQALADGINNFSQAKAASVKVNADAPGSFYCGCQIRWQGKKGVVDLESCGYKVRKNENRARRI
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPAIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKAK
IAEPPARARGAIARTYFYMRDQYQLKLSRQQTQLFNVWDKQYPVTAWECERDARIAKVQGNHNPYVQRACQARKS
>Mature_235_residues
MYRNFSFAAALLAAAFSGQALADGINNFSQAKAASVKVNADAPGSFYCGCQIRWQGKKGVVDLESCGYKVRKNENRARRI
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPAIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKAK
IAEPPARARGAIARTYFYMRDQYQLKLSRQQTQLFNVWDKQYPVTAWECERDARIAKVQGNHNPYVQRACQARKS

Specific function: Has double-strand break activity [H]

COG id: COG2356

COG function: function code L; Endonuclease I

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endA/nucM nuclease family [H]

Homologues:

Organism=Escherichia coli, GI1789314, Length=235, Percent_Identity=87.6595744680851, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007346 [H]

Pfam domain/function: PF04231 Endonuclease_1 [H]

EC number: =3.1.21.1 [H]

Molecular weight: Translated: 26794; Mature: 26794

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRNFSFAAALLAAAFSGQALADGINNFSQAKAASVKVNADAPGSFYCGCQIRWQGKKGV
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEEECCCCCC
VDLESCGYKVRKNENRARRIEWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEEECCCCHHHEEEHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPAIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKAKIAEPPARARGAIARTYFYMR
HCCHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEECEEECCCCCHHHHHHHHHHHHHHC
DQYQLKLSRQQTQLFNVWDKQYPVTAWECERDARIAKVQGNHNPYVQRACQARKS
CCEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MYRNFSFAAALLAAAFSGQALADGINNFSQAKAASVKVNADAPGSFYCGCQIRWQGKKGV
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEEECCCCCC
VDLESCGYKVRKNENRARRIEWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEEECCCCHHHEEEHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPAIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKAKIAEPPARARGAIARTYFYMR
HCCHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEECEEECCCCCHHHHHHHHHHHHHHC
DQYQLKLSRQQTQLFNVWDKQYPVTAWECERDARIAKVQGNHNPYVQRACQARKS
CCEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867949; 9278503; 4955462; 8113204 [H]