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The map label for this gene is ackA [H]

Identifier: 194736648

GI number: 194736648

Start: 2451038

End: 2452240

Strand: Direct

Name: ackA [H]

Synonym: SeSA_A2566

Alternate gene names: 194736648

Gene position: 2451038-2452240 (Clockwise)

Preceding gene: 194738246

Following gene: 194737608

Centisome position: 52.05

GC content: 52.12

Gene sequence:

>1203_bases
ATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCAATTATTGATGCAGTTAATGGTGA
TGAATACCTTTCTGGTTTAGCCGAATGTTTCCATCTGCCAGAAGCACGCATCAAATGGAAAATGGACGGCAGTAAACAAG
AAGCGGCTTTAGGTGCAGGCGCCGCTCACAGTGAAGCGCTGAACTTTATCGTTAACACTATTCTGGCACAAAAACCAGAA
CTGTCTGCGCAGTTAACTGCAATTGGTCACCGTATCGTACACGGCGGCGAGAAGTATACCAGTTCCGTAGTCATTGATGA
GTCCGTCATTCAGGGCATCAAAGATTCCGCCTCTTTCGCACCGCTGCATAACCCGGCTCACCTGATTGGTATCGCCGAAG
CGCTGAAATCTTTCCCGCAGCTGAAAGATAAAAACGTTGCAGTATTCGACACCGCCTTCCATCAGACCATGCCGGAAGAA
TCTTACCTGTATGCCCTGCCGTACAGCCTGTATAAAGAACACGGCGTTCGTCGCTATGGCGCACACGGCACCAGCCACTT
CTATGTAACTCAGGAAGCGGCAAAAATGCTGAACAAGCCGGTAGAAGAACTGAACATTATCACCTGCCACCTGGGCAACG
GCGGTTCTGTTTCTGCTATCCGTAACGGTAAATGCGTGGATACCTCTATGGGTCTGACCCCGCTGGAAGGCCTGGTCATG
GGTACCCGTTCCGGCGATATCGACCCTGCGATTATCTTCCACCTGCACGACACTCTGGGCATGAGCGTAGATCAGATCAA
CAAAATGCTGACCAAAGAGTCCGGTCTGCTGGGTCTGACTGAAGTGACCAGCGACTGCCGTTATGTTGAAGACAACTACG
CCACCAAAGAAGACGCAAAACGTGCGATGGACGTTTACTGCCACCGCCTGGCGAAGTACATCGGTTCTTACACCGCTCTG
ATGGACGGTCGTCTGGATGCAGTCGTGTTCACCGGCGGTATCGGTGAAAACGCCGCGATGGTTCGCGAACTGTCTCTGGG
TAAACTGGGCGTGCTGGGCTTTGAAGTCGACCACGAACGTAACCTGGCGGCCCGCTTCGGCAAATCTGGTTTCATCAACA
AAGAAGGTACCCGTCCGGCGGTGGTGATCCCAACCAACGAAGAACTGGTTATCGCGCAGGACGCGAGCCGTCTGACTGCC
TGA

Upstream 100 bases:

>100_bases
TCCTGCGCATGATGTTAATCATAAATGTCAGGGCTATCATGCGCTACGCTCTATGGCTCACTGACGTTTTTTTAGCCACG
TATCATAAATAGGTACTTCC

Downstream 100 bases:

>100_bases
TTTGACACCGCCAGCCTCGCTGGCGGTGCTGTTTTGTAACCCGTCTCCAAAAGACGGTAACGAAAGAGGATAAACCGTGT
CCCGTATTATTATGCTGATC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPEL
SAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEES
YLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTALM
DGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=97.75, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=43.6868686868687, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43258; Mature: 43127

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHCCCCCC
PLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHCCCCCC
PLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]