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The map label for this gene is phrB [H]

Identifier: 194736598

GI number: 194736598

Start: 840578

End: 841999

Strand: Direct

Name: phrB [H]

Synonym: SeSA_A0866

Alternate gene names: 194736598

Gene position: 840578-841999 (Clockwise)

Preceding gene: 194738269

Following gene: 194735431

Centisome position: 17.85

GC content: 56.75

Gene sequence:

>1422_bases
ATGCCCACCCATTTAGTCTGGTTCAGGCGCGATCTGCGTTTACAGGATAACCTTGCGCTGGCCGCCGCCTGCCGCGATGC
ATCCGCGCGAGTGCTGGCGCTTTATATTTCCACCCCCGCGCAGTGGCAGGCTCATGATATGGCGCCGCGGCAGGCGGCAT
TCATCAGCGCGCAGCTCAATGCGCTACAGACGGCCCTTGCAGAGAAAGGGATTCCGCTGCTGTTTCATGAAGTGGCGGAT
TTTAACGCCAGTATTGAAACGGTCAAAAACGTTTGCCGACAGCATGACGTCAGCCATCTGTTTTATAACTATCAGTATGA
GTTTAACGAGCGTCAGCGTGATGCGGCGGTGGAAAAAACGCTGCCCTCTGTCATCTGCGAAGGCTTTGACGATAGCGTGA
TTCTGGCGCCCGGCGCGGTGATGACCGGCAATCATGAAATGTATAAAGTTTTTACGCCGTTTAAAAACGCCTGGTTGAAA
CGGCTAAAAGAGGATATCCCGCCGTGCGTTCCGGCCCCGAAGATCCGGGTGAGCGGCGCGCTTTCTACACCCTTAACGCC
AGTCTCGCTTAACTACCCGCAACAGGCGTTTGACGCCGCGCTTTTCCCGGTGGAAGAAAACGCGGTTATCGCGCAGCTAC
GTCAGTTTTGCGCGCAGGGCGCAGACGAGTATGAGTCACAGCGGGATTTCCCTGCGGTCGACGGCACCAGTCGGCTTTCC
GCCAGCCTGGCGACGGGTGGCCTGTCGCCGCGACAGTGCCTGCACCGATTACTGGCGGAGCAGCCGCAGGCGCTGGACGG
CGGACCGGGAAGCGTCTGGTTGAATGAACTCATCTGGCGGGAATTTTACCGTCATTTAATGACCTGGTATCCGGCATTAT
GCAAACACCAGCCGTTTATCCGCTGGACAAAACGCGTCGCGTGGCAGGAGAACCCGCACTATTTTCAGGCATGGCAGAAA
GGCGAAACCGGTTATCCCATTGTCGATGCGGCGATGCGCCAGCTTAACGCGACGGGCTGGATGCATAACCGTTTACGCAT
GATTACCGCCAGCTTTCTGGTAAAAGATCTGTTGATTGACTGGCGGTTGGGGGAGCGCTATTTCATGTCGCAGCTTATTG
ACGGCGATCTTGCCGCCAATAATGGCGGCTGGCAGTGGGCCGCCTCAACCGGTACTGATGCCGCGCCTTATTTTCGTATT
TTTAATCCCACGACTCAGGGAGAGAGGTTCGATCGCGACGGCGAATTTATCCGCCAGTGGCTACCGGCATTACGCGATAT
CCCTGGAAAAGCGATTCACGAGCCGTGGCGGTGGGCGGAAAAAGCGGGAGTCGTGCTTGATTATCCTCGGCCCATTGTTG
AACACAAACAGGCGAGAATCGCGACGCTTTCCGCCTATGAGGCGGCGAGAAAAGGGGCGTGA

Upstream 100 bases:

>100_bases
CCTGAAAAACCGTCAGCCGCCACGCTGGCGGTTTTTTTATGCGCCAGGGTCTACACTGTTTAACGCTATTCGCCGTGACG
TTTCATCTTTCAGGAGTTGT

Downstream 100 bases:

>100_bases
TACAGGCCCGGTAGCGCAAACTACCGGGCCTTCTGCTATCAGGATTCGACGGCCAGCGCGCGGCTTTTGAATTTGAAGGA
CTGATACAGCCAAATCAGCA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVAD
FNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLK
RLKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYESQRDFPAVDGTSRLS
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQK
GETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRI
FNPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA

Sequences:

>Translated_473_residues
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVAD
FNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLK
RLKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYESQRDFPAVDGTSRLS
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQK
GETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRI
FNPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
>Mature_472_residues
PTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVADF
NASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKR
LKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYESQRDFPAVDGTSRLSA
SLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQKG
ETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIF
NPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=498, Percent_Identity=26.9076305220884, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI4758072, Length=492, Percent_Identity=25.2032520325203, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI188536103, Length=286, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1786926, Length=472, Percent_Identity=79.6610169491525, Blast_Score=806, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324962, Length=506, Percent_Identity=33.201581027668, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17137248, Length=502, Percent_Identity=27.4900398406374, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24585455, Length=502, Percent_Identity=27.4900398406374, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24648152, Length=526, Percent_Identity=24.1444866920152, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53680; Mature: 53548

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLN
CCCCEEEHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHH
ALQTALAEKGIPLLFHEVADFNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKT
HHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHH
LPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKRLKEDIPPCVPAPKIRVSGA
HHHHHHCCCCCCEEECCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEECCC
LSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYESQRDFPAVDGTSRLS
CCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFI
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWTKRVAWQENPHYFQAWQKGETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLID
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
WRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDRDGEFIRQW
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
HHHHHCCCCHHHCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLN
CCCEEEHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHH
ALQTALAEKGIPLLFHEVADFNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKT
HHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHH
LPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKRLKEDIPPCVPAPKIRVSGA
HHHHHHCCCCCCEEECCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEECCC
LSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYESQRDFPAVDGTSRLS
CCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFI
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWTKRVAWQENPHYFQAWQKGETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLID
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
WRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDRDGEFIRQW
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
HHHHHCCCCHHHCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609 [H]