Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 194736596

GI number: 194736596

Start: 4245107

End: 4245865

Strand: Reverse

Name: thiF [H]

Synonym: SeSA_A4372

Alternate gene names: 194736596

Gene position: 4245865-4245107 (Counterclockwise)

Preceding gene: 194733947

Following gene: 194737963

Centisome position: 90.16

GC content: 59.03

Gene sequence:

>759_bases
ATGAATGACCGTGACTTTATGCGTTATAGCCGCCAGATCCTGCTCGGCGATATAGCCATTGAAGGCCAGCAAAAGTTGCT
CGCGAGTCATGTCCTGATCGTGGGTTTAGGCGGGTTGGGTTCACCCGCCGCGCTGTATCTGACGGGAGCAGGCATCGGCA
AACTGACGCTGGTAGACGATGACGACATTCATCTGAGCAATTTGCAGCGCCAGATTCTATTTACCACCGATGATATCGCA
GGTTCAAAATCTCAGGTTGCCCGGCAGCGCCTGACACGGCTCAACCCGGATATCGAACTGGTGTCGCTCCAACAGCGGCT
GCAAGGCGAGGCGCTCCGACATGCGGTCGCGCGTGCCGACGTAGTGCTCGACTGTACCGACAACATGGCCACGCGCCAGG
AAATTAACGCCGCCTGCGTGGCGCTCAACACTCCGTTAATTTCCGCCAGCGCCGTCGGCTTTGGCGGCCAGCTAATGGTC
CTCACACCACCGTGGGAACAAGGCTGTTACCGCTGCCTGTGGCCGGACGATGTCGAGCCTGAACGCAACTGCCGTACCGC
CGGTATCGTCGGACCGGTTGTTGGCGTGATGGGCACTTTGCAGGCGCTGGAGGCAATCAAATTACTCAGCGGGATGGAAA
CGCCGAGTGGCGAGCTACGCCTGTTTGACGGTAAAACCAGCCAGTGGCGCAGCCTGGCGCTGCGTCGTGCCAGCGGCTGT
CCGGTCTGCGGAGGGCAACATGCAGATTCATTTCAATGA

Upstream 100 bases:

>100_bases
GCGACCGGCGTCGGCAGTATTGCCGTGGTCAGCGCCATTACTCAGGCCGCCGACTGGCGCGCCGCTACCGCGCAGTTACT
GGATATTGCGGGAGTTGGCG

Downstream 100 bases:

>100_bases
TGAACCGATGCAGTGCGCCGCAGGGCAAACCGTCAATGAGCTGCTTGCTGCGCTTAATCAGCTTAAACCGGGCGCGGCGC
TGGCGCTCAATCAGCAGATC

Product: adenylyltransferase ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNDRDFMRYSRQILLGDIAIEGQQKLLASHVLIVGLGGLGSPAALYLTGAGIGKLTLVDDDDIHLSNLQRQILFTTDDIA
GSKSQVARQRLTRLNPDIELVSLQQRLQGEALRHAVARADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGMETPSGELRLFDGKTSQWRSLALRRASGC
PVCGGQHADSFQ

Sequences:

>Translated_252_residues
MNDRDFMRYSRQILLGDIAIEGQQKLLASHVLIVGLGGLGSPAALYLTGAGIGKLTLVDDDDIHLSNLQRQILFTTDDIA
GSKSQVARQRLTRLNPDIELVSLQQRLQGEALRHAVARADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGMETPSGELRLFDGKTSQWRSLALRRASGC
PVCGGQHADSFQ
>Mature_252_residues
MNDRDFMRYSRQILLGDIAIEGQQKLLASHVLIVGLGGLGSPAALYLTGAGIGKLTLVDDDDIHLSNLQRQILFTTDDIA
GSKSQVARQRLTRLNPDIELVSLQQRLQGEALRHAVARADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGMETPSGELRLFDGKTSQWRSLALRRASGC
PVCGGQHADSFQ

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=224, Percent_Identity=44.1964285714286, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI4885649, Length=143, Percent_Identity=31.4685314685315, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI38045948, Length=160, Percent_Identity=33.75, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI13376212, Length=184, Percent_Identity=33.695652173913, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=85.9437751004016, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=44.8979591836735, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789177, Length=152, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=41.9354838709677, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=154, Percent_Identity=33.1168831168831, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI86565433, Length=150, Percent_Identity=28.6666666666667, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI86565431, Length=150, Percent_Identity=28.6666666666667, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=248, Percent_Identity=37.0967741935484, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320598, Length=161, Percent_Identity=31.6770186335404, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322639, Length=170, Percent_Identity=27.6470588235294, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=44.6215139442231, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28573937, Length=164, Percent_Identity=28.6585365853659, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641311, Length=182, Percent_Identity=32.4175824175824, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 27194; Mature: 27194

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRDFMRYSRQILLGDIAIEGQQKLLASHVLIVGLGGLGSPAALYLTGAGIGKLTLVDD
CCCHHHHHHHHHHEEEEEEECCHHHHHHHHEEEEEECCCCCCEEEEEEECCCCEEEEECC
DDIHLSNLQRQILFTTDDIAGSKSQVARQRLTRLNPDIELVSLQQRLQGEALRHAVARAD
CCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
VVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMVLTPPWEQGCYRCLWPDDVEP
EEEECCCCHHHHHHHCEEEEEECCCCEEEEECCCCCEEEEECCCHHHCCEEECCCCCCCC
ERNCRTAGIVGPVVGVMGTLQALEAIKLLSGMETPSGELRLFDGKTSQWRSLALRRASGC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
PVCGGQHADSFQ
CCCCCCCCCCCC
>Mature Secondary Structure
MNDRDFMRYSRQILLGDIAIEGQQKLLASHVLIVGLGGLGSPAALYLTGAGIGKLTLVDD
CCCHHHHHHHHHHEEEEEEECCHHHHHHHHEEEEEECCCCCCEEEEEEECCCCEEEEECC
DDIHLSNLQRQILFTTDDIAGSKSQVARQRLTRLNPDIELVSLQQRLQGEALRHAVARAD
CCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
VVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMVLTPPWEQGCYRCLWPDDVEP
EEEECCCCHHHHHHHCEEEEEECCCCEEEEECCCCCEEEEECCCHHHCCEEECCCCCCCC
ERNCRTAGIVGPVVGVMGTLQALEAIKLLSGMETPSGELRLFDGKTSQWRSLALRRASGC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
PVCGGQHADSFQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]