Click here to switch to the map view.

The map label for this gene is mrkB [H]

Identifier: 194736580

GI number: 194736580

Start: 391751

End: 392509

Strand: Reverse

Name: mrkB [H]

Synonym: SeSA_A0389

Alternate gene names: 194736580

Gene position: 392509-391751 (Counterclockwise)

Preceding gene: 194738316

Following gene: 194736139

Centisome position: 8.34

GC content: 46.9

Gene sequence:

>759_bases
ATGCGCAATTACAGGCAGTGGTTAGTTTTCAGTAAAGTCATATTAACGCTACTTGGGTTGACGGGATTGTATGGCCCGGC
ACAAGCCGCCGTTAATATTGACAGAACCCGAATTATTTTTGCTTCAGATGATATCGCTCAATCGTTGACATTATCCAACG
ATAATACAACGCCGATGCTGTTACAGGTATGGACCGACGCCGGAAACATTGACGCCTCTCCGGATAATAGCAAAACGCCG
CTGGTGGCGCTGCCGCCGGTATTTAAAATGCAGCCAGGGGAACTGAGAACGCTTCGCCTGCTCCTGAGTTCCCGCCAGCA
GCTTGCAACGGACCGGGAGAGTCTCTTCTGGCTAAACATTTATCAGATACCGCCACTCCCCCATGACATTAAAAACAACC
CGCGAAAACTGGTATTACCGCTTCGTTTGCGGCTAAAAATTTTAATTCGCCCGACCGGGCTAAAAGCGCCAACGGAAGCG
GAGGAGAAAAAATTACGTTTTATTGCTAAAGAGAATACTATACGGATAGTTAACCCCACCTCGTGGTATATGAGTTTAAC
GCTAACGACGGATGACAAGAAAAGTATCGGCGATATCATGGTTGCGCCGAAAACAGCACTTGATGTGCCGCTAACACGCT
CGCCAACGCCGGGAGCATCGATAAATTATGCAGTAATCAATGACTCCGGGAACTGGCGACAATATACCGCCATACTGGAA
AAATATATTAGTAAGAGTGATTTTATTCCTGAGTTTTGA

Upstream 100 bases:

>100_bases
ACCACACGAGTATCATCCGGTTCAGTAGAAACCTACAGCGGTGATTTTACTGCCTCGCTTGAGGCGATCGCCGGACAAAC
AGTAACGGCAGGAAGCGTGA

Downstream 100 bases:

>100_bases
TGGGAAATATTCAGGGATACGGTCGTATCCCTGAGACAAGCCAGAATTCAGCATGTACCATTGTCAAAAATAGCATTAAG
CTGATATTTTTCTTTTTGCA

Product: fimbrial chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRNYRQWLVFSKVILTLLGLTGLYGPAQAAVNIDRTRIIFASDDIAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPLPHDIKNNPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTTDDKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSDFIPEF

Sequences:

>Translated_252_residues
MRNYRQWLVFSKVILTLLGLTGLYGPAQAAVNIDRTRIIFASDDIAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPLPHDIKNNPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTTDDKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSDFIPEF
>Mature_252_residues
MRNYRQWLVFSKVILTLLGLTGLYGPAQAAVNIDRTRIIFASDDIAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPLPHDIKNNPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTTDDKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSDFIPEF

Specific function: Mediates assembly of pili by forming soluble multimeric complexes with pili subunits as an intermediate step in the assembly process. This protein is involved in type 3 pili assembly [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=228, Percent_Identity=30.7017543859649, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790771, Length=207, Percent_Identity=31.8840579710145, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789532, Length=222, Percent_Identity=30.6306306306306, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=29.9539170506912, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786743, Length=207, Percent_Identity=28.5024154589372, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787171, Length=216, Percent_Identity=25.462962962963, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788428, Length=239, Percent_Identity=25.9414225941423, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082203, Length=239, Percent_Identity=25.1046025104602, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788677, Length=192, Percent_Identity=29.6875, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081806, Length=228, Percent_Identity=28.5087719298246, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 28428; Mature: 28428

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNYRQWLVFSKVILTLLGLTGLYGPAQAAVNIDRTRIIFASDDIAQSLTLSNDNTTPML
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCEEEEEECCCHHHEEEECCCCCCEEE
LQVWTDAGNIDASPDNSKTPLVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNI
EEEEECCCCCCCCCCCCCCCEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
YQIPPLPHDIKNNPRKLVLPLRLRLKILIRPTGLKAPTEAEEKKLRFIAKENTIRIVNPT
EECCCCCHHHCCCCEEEEEEEEEEEEEEEEECCCCCCCCHHHHHEEEEEECCEEEEECCC
SWYMSLTLTTDDKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
CEEEEEEEECCCCCCCCCEEECCCHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KYISKSDFIPEF
HHHCCCCCCCCC
>Mature Secondary Structure
MRNYRQWLVFSKVILTLLGLTGLYGPAQAAVNIDRTRIIFASDDIAQSLTLSNDNTTPML
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCEEEEEECCCHHHEEEECCCCCCEEE
LQVWTDAGNIDASPDNSKTPLVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNI
EEEEECCCCCCCCCCCCCCCEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
YQIPPLPHDIKNNPRKLVLPLRLRLKILIRPTGLKAPTEAEEKKLRFIAKENTIRIVNPT
EECCCCCHHHCCCCEEEEEEEEEEEEEEEEECCCCCCCCHHHHHEEEEEECCEEEEECCC
SWYMSLTLTTDDKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
CEEEEEEEECCCCCCCCCEEECCCHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KYISKSDFIPEF
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1670938 [H]