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The map label for this gene is silA [H]

Identifier: 194736238

GI number: 194736238

Start: 701942

End: 702157

Strand: Direct

Name: silA [H]

Synonym: SeSA_A0728

Alternate gene names: 194736238

Gene position: 701942-702157 (Clockwise)

Preceding gene: 194737010

Following gene: 194738130

Centisome position: 14.91

GC content: 62.04

Gene sequence:

>216_bases
GTGCTCCGGGTGCGGCCAAAAGCCATGACGGTAGCGGTGATTATCGCCGGTCTGCTGCCTTTTTTATGGGGGGCTGGCGC
GGGTTCTGAGGTGATGAGCCGTATTGCCGCGCCGATGGTCGGCGGCATGATTACCGCGCCGCTGCTGTCGCTGTTCATCA
TTCCGGCAGCCTACAAGCTGATGTGGCTGCGCAGGCATCGTCGCCTTGCGGCATAA

Upstream 100 bases:

>100_bases
TGATGGGATCGCCGGTGATCCAGCAGGCGCGGCCTTCAAAGCGCAAGCTCCCAACACCTTTTCGGCAGAAAAACTCGATG
AGACGCTTTACCACGGGGCG

Downstream 100 bases:

>100_bases
CGTGTGCACCCCGCCTGCGGCGTCAAACTGCAGGCGGGTTCAGCCTACGTTTATCGTCCGAGTGCGTTGACCCGTTTGCG
GTATTCGCCTGGTGTACAAC

Product: cation efflux system protein CusA

Products: Proton [Cytoplasm]; silver [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 71; Mature: 71

Protein sequence:

>71_residues
MLRVRPKAMTVAVIIAGLLPFLWGAGAGSEVMSRIAAPMVGGMITAPLLSLFIIPAAYKLMWLRRHRRLAA

Sequences:

>Translated_71_residues
MLRVRPKAMTVAVIIAGLLPFLWGAGAGSEVMSRIAAPMVGGMITAPLLSLFIIPAAYKLMWLRRHRRLAA
>Mature_71_residues
MLRVRPKAMTVAVIIAGLLPFLWGAGAGSEVMSRIAAPMVGGMITAPLLSLFIIPAAYKLMWLRRHRRLAA

Specific function: Component of the sil cation-efflux system that confers resistance to silver. May be part of a three-component cation/proton antiporter [H]

COG id: COG3696

COG function: function code P; Putative silver efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1786788, Length=67, Percent_Identity=92.5373134328358, Blast_Score=126, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004763 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 7702; Mature: 7702

Theoretical pI: Translated: 12.63; Mature: 12.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
8.5 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
8.5 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVRPKAMTVAVIIAGLLPFLWGAGAGSEVMSRIAAPMVGGMITAPLLSLFIIPAAYKL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWLRRHRRLAA
HHHHHHHHCCC
>Mature Secondary Structure
MLRVRPKAMTVAVIIAGLLPFLWGAGAGSEVMSRIAAPMVGGMITAPLLSLFIIPAAYKL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWLRRHRRLAA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; silver [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + silver [Cytoplasm] = Proton [Cytoplasm] + silver [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]