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The map label for this gene is prpE [H]

Identifier: 194736206

GI number: 194736206

Start: 434460

End: 436346

Strand: Direct

Name: prpE [H]

Synonym: SeSA_A0423

Alternate gene names: 194736206

Gene position: 434460-436346 (Clockwise)

Preceding gene: 194736852

Following gene: 194735645

Centisome position: 9.23

GC content: 58.82

Gene sequence:

>1887_bases
ATGTCTTTTAGCGAATTTTATCAGCGTTCCATTAACGAACCGGAGGCGTTCTGGGCCGAGCAGGCCCGGCGTATCGACTG
GCGACAGCCGTTTACGCAGACGCTGGATCATAGCCGTCCACCTTTTGCCCGCTGGTTTTGCGGCGGCACCACTAACTTAT
GTCATAACGCCGTCGACCGCTGGCGGGATAAGCAGCCGGAGGCGCTGGCGCTGATTGCCGTCTCATCAGAGACCGATGAA
GAGCGCACATTTACCTTCAGCCAGTTGCATGATGAAGTCAACGCTGTGGCCGCTATGCTGCTGTCGCTGGGCGTGCAGCG
TGGCGATCGCGTATTGGTCTATATGCCGATGATTGCCGAAGCGCAGATAACCCTGCTGGCCTGCGCGCGCATTGGCGCGA
TCCATTCGGTGGTCTTTGGCGGTTTTGCCTCGCACAGCGTGGCGGCGCGCATTGACGATGCCAGACCGGCGCTGATTGTG
TCGGCGGATGCCGGAGCGCGGGGCGGTAAAATCCTGCCGTATAAAAAGCTGCTTGATGACGCTATTGCGCAGGCGCAGCA
TCAGCCGAAACATGTTCTGCTGGTGGACAGAGGGCTGGCGAAAATGGCGTGGGTGGATGGGCGCGATCTGGATTTTGCCA
CGTTGCGCCAGCAGCATCTCGGCGCGAGCGTGCCGGTGGCGTGGCTGGAATCCAATGAAACCTCATGCATTCTTTACACC
TCCGGCACTACCGGCAAACCGAAAGGCGTCCAGCGCGACGTTGGCGGTTATGCGGTGGCGCTGGCAACCTCGATGGACAC
CATTTTTGGCGGCAAGGCGGGCGGCGTATTCTTTTGCGCATCGGATATCGGCTGGGTCGTCGGCCACTCCTATATCGTTT
ACGCGCCGCTGCTCGCAGGCATGGCGACTATTGTTTACGAAGGACTGCCGACGTACCCGGACTGCGGGGTCTGGTGGAAA
ATTGTCGAGAAATACCAGGTTAACCGGATGTTTTCCGCCCCGACCGCGATTCGCGTGCTGAAAAAATTCCCGACGGCGCA
AATCCGCAATCACGATCTCTCCTCGCTGGAGGCGCTTTATCTGGCCGGTGAGCCGCTGGACGAGCCGACGGCCAGTTGGG
TAACGGAGACGCTGGGTGTGCCGGTTATCGACAATTATTGGCAGACGGAGTCCGGCTGGCCGATCATGGCGCTGGCCCGC
GCGCTGGACGACAGGCCGTCGCGTCTGGGAAGTCCCGGCGTGCCGATGTACGGTTATAACGTCCAGCTACTCAATGAAGT
CACCGGCGACCCCTGCGGCATAAATGAAAAAGGCATGCTGGTGATTGAGGGGCCGCTGCCGCCGGGCTGTATTCAGACTA
TTTGGGGCGACGATGCGCGTTTTGTGAAGACTTACTGGTCGCTGTTTAACCGTCAGGTTTATGCCACCTTCGACTGGGGA
ATCCGCGATGCCGAGGGGTATTACTTTATTCTGGGCCGTACCGATGATGTGATTAATATTGCGGGCCATCGGCTGGGGAC
GCGAGAAATAGAAGAAAGTATCTCCGGTTACCCGAACGTAGCGGAAGTGGCGGTGGTGGGGATAAAGGACGCTCTGAAAG
GGCAGGTAGCGGTGGCGTTTGTCATTCCGAAGCAGAGCGATACGCTGGCGGATCGCGAGGCGGCGCGCGACGAGGAAAAA
GCGATTATGGCGCTGGTGGACAACCAGATCGGTCACTTTGGTCGTCCGGCGCATGTCTGGTTTGTTTCGCAGCTCCCCAA
AACGCGTTCCGGAAAGATGCTTCGCCGCACGATCCAGGCGATCTGTGAAGGCCGAGATCCGGGCGATCTGACAACTATTG
ACGATCCCGCGTCGTTGCAGCAAATTCGCCAGGCGATCGAGGAATAG

Upstream 100 bases:

>100_bases
GACAGAGCCCGCCTGGAGCAGATGCCGGTTAACGAATACCTCGATTTATATGTCATCTGAGCCAGCCAGCAGTAAGGCGT
AAGTTCAACAGGAGAGCGTG

Downstream 100 bases:

>100_bases
CCGTAAGCCGGATAAGCCGCGTCCGGCAACGGGTTAGCGTCAGCGACTGAATAGTGGGGTGTAAAGTTGGGAAAGGGCGG
CAGCGCGCCGCCCTGTAACA

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSFSEFYQRSINEPEAFWAEQARRIDWRQPFTQTLDHSRPPFARWFCGGTTNLCHNAVDRWRDKQPEALALIAVSSETDE
ERTFTFSQLHDEVNAVAAMLLSLGVQRGDRVLVYMPMIAEAQITLLACARIGAIHSVVFGGFASHSVAARIDDARPALIV
SADAGARGGKILPYKKLLDDAIAQAQHQPKHVLLVDRGLAKMAWVDGRDLDFATLRQQHLGASVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWK
IVEKYQVNRMFSAPTAIRVLKKFPTAQIRNHDLSSLEALYLAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALAR
ALDDRPSRLGSPGVPMYGYNVQLLNEVTGDPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYATFDWG
IRDAEGYYFILGRTDDVINIAGHRLGTREIEESISGYPNVAEVAVVGIKDALKGQVAVAFVIPKQSDTLADREAARDEEK
AIMALVDNQIGHFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLQQIRQAIEE

Sequences:

>Translated_628_residues
MSFSEFYQRSINEPEAFWAEQARRIDWRQPFTQTLDHSRPPFARWFCGGTTNLCHNAVDRWRDKQPEALALIAVSSETDE
ERTFTFSQLHDEVNAVAAMLLSLGVQRGDRVLVYMPMIAEAQITLLACARIGAIHSVVFGGFASHSVAARIDDARPALIV
SADAGARGGKILPYKKLLDDAIAQAQHQPKHVLLVDRGLAKMAWVDGRDLDFATLRQQHLGASVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWK
IVEKYQVNRMFSAPTAIRVLKKFPTAQIRNHDLSSLEALYLAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALAR
ALDDRPSRLGSPGVPMYGYNVQLLNEVTGDPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYATFDWG
IRDAEGYYFILGRTDDVINIAGHRLGTREIEESISGYPNVAEVAVVGIKDALKGQVAVAFVIPKQSDTLADREAARDEEK
AIMALVDNQIGHFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLQQIRQAIEE
>Mature_627_residues
SFSEFYQRSINEPEAFWAEQARRIDWRQPFTQTLDHSRPPFARWFCGGTTNLCHNAVDRWRDKQPEALALIAVSSETDEE
RTFTFSQLHDEVNAVAAMLLSLGVQRGDRVLVYMPMIAEAQITLLACARIGAIHSVVFGGFASHSVAARIDDARPALIVS
ADAGARGGKILPYKKLLDDAIAQAQHQPKHVLLVDRGLAKMAWVDGRDLDFATLRQQHLGASVPVAWLESNETSCILYTS
GTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTYPDCGVWWKI
VEKYQVNRMFSAPTAIRVLKKFPTAQIRNHDLSSLEALYLAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALARA
LDDRPSRLGSPGVPMYGYNVQLLNEVTGDPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYATFDWGI
RDAEGYYFILGRTDDVINIAGHRLGTREIEESISGYPNVAEVAVVGIKDALKGQVAVAFVIPKQSDTLADREAARDEEKA
IMALVDNQIGHFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLQQIRQAIEE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=633, Percent_Identity=39.3364928909953, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI8923896, Length=657, Percent_Identity=35.1598173515982, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=37.1875, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=34.3283582089552, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI42544132, Length=556, Percent_Identity=26.4388489208633, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI115511026, Length=560, Percent_Identity=27.3214285714286, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI122937307, Length=559, Percent_Identity=26.4758497316637, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI58082049, Length=565, Percent_Identity=26.3716814159292, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI38505220, Length=556, Percent_Identity=25.3597122302158, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI157311624, Length=565, Percent_Identity=26.1946902654867, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI157311622, Length=565, Percent_Identity=26.1946902654867, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI31982927, Length=641, Percent_Identity=23.2449297971919, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI42544134, Length=369, Percent_Identity=23.5772357723577, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI156151445, Length=574, Percent_Identity=23.1707317073171, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI187761345, Length=413, Percent_Identity=23.728813559322, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI187761343, Length=413, Percent_Identity=23.728813559322, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786529, Length=628, Percent_Identity=89.8089171974522, Blast_Score=1166, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=638, Percent_Identity=38.0877742946708, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI221142682, Length=550, Percent_Identity=24.7272727272727, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693145, Length=553, Percent_Identity=21.5189873417722, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.3396584440228, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788107, Length=562, Percent_Identity=23.3096085409253, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789201, Length=487, Percent_Identity=23.4086242299795, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=653, Percent_Identity=33.6906584992343, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71983001, Length=653, Percent_Identity=33.6906584992343, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17569507, Length=646, Percent_Identity=20.8978328173375, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=552, Percent_Identity=24.4565217391304, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=573, Percent_Identity=21.9895287958115, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=556, Percent_Identity=22.1223021582734, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=19.3308550185874, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=19.3308550185874, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=19.3308550185874, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=541, Percent_Identity=20.7024029574861, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319264, Length=643, Percent_Identity=37.9471228615863, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323182, Length=645, Percent_Identity=37.0542635658915, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319699, Length=383, Percent_Identity=22.976501305483, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355225, Length=643, Percent_Identity=36.547433903577, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24667955, Length=651, Percent_Identity=34.8694316436252, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI62472339, Length=566, Percent_Identity=35.8657243816254, Blast_Score=358, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24667959, Length=530, Percent_Identity=35.6603773584906, Blast_Score=331, Evalue=1e-90,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=24.007561436673, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358303, Length=503, Percent_Identity=21.8687872763419, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648260, Length=377, Percent_Identity=27.5862068965517, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922652, Length=387, Percent_Identity=25.5813953488372, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=369, Percent_Identity=23.5772357723577, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130357, Length=380, Percent_Identity=24.7368421052632, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=374, Percent_Identity=24.331550802139, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69263; Mature: 69131

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEFYQRSINEPEAFWAEQARRIDWRQPFTQTLDHSRPPFARWFCGGTTNLCHNAVDR
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHEEEECCCHHHHHHHHHH
WRDKQPEALALIAVSSETDEERTFTFSQLHDEVNAVAAMLLSLGVQRGDRVLVYMPMIAE
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
AQITLLACARIGAIHSVVFGGFASHSVAARIDDARPALIVSADAGARGGKILPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCHHCCHHEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
AIAQAQHQPKHVLLVDRGLAKMAWVDGRDLDFATLRQQHLGASVPVAWLESNETSCILYT
HHHHHHCCCCEEEEEECCCHHHEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTYPDCGVWWKIVEKYQVNRMFSAPTAIRVLKKFPTAQIRNHDLSSLEALY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCHHHHHEEE
LAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALARALDDRPSRLGSPGVPMYGYN
ECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCEECCC
VQLLNEVTGDPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYATFDWG
CEEEHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECC
IRDAEGYYFILGRTDDVINIAGHRLGTREIEESISGYPNVAEVAVVGIKDALKGQVAVAF
CCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHCCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKQSDTLADREAARDEEKAIMALVDNQIGHFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLQQIRQAIEE
HHCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SFSEFYQRSINEPEAFWAEQARRIDWRQPFTQTLDHSRPPFARWFCGGTTNLCHNAVDR
CHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHEEEECCCHHHHHHHHHH
WRDKQPEALALIAVSSETDEERTFTFSQLHDEVNAVAAMLLSLGVQRGDRVLVYMPMIAE
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
AQITLLACARIGAIHSVVFGGFASHSVAARIDDARPALIVSADAGARGGKILPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCHHCCHHEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
AIAQAQHQPKHVLLVDRGLAKMAWVDGRDLDFATLRQQHLGASVPVAWLESNETSCILYT
HHHHHHCCCCEEEEEECCCHHHEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTYPDCGVWWKIVEKYQVNRMFSAPTAIRVLKKFPTAQIRNHDLSSLEALY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCHHHHHEEE
LAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALARALDDRPSRLGSPGVPMYGYN
ECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCEECCC
VQLLNEVTGDPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYATFDWG
CEEEHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECC
IRDAEGYYFILGRTDDVINIAGHRLGTREIEESISGYPNVAEVAVVGIKDALKGQVAVAF
CCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHCCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKQSDTLADREAARDEEKAIMALVDNQIGHFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLQQIRQAIEE
HHCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]