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The map label for this gene is ubiF [H]

Identifier: 194736144

GI number: 194736144

Start: 800935

End: 802110

Strand: Direct

Name: ubiF [H]

Synonym: SeSA_A0825

Alternate gene names: 194736144

Gene position: 800935-802110 (Clockwise)

Preceding gene: 194736800

Following gene: 194736511

Centisome position: 17.01

GC content: 59.86

Gene sequence:

>1176_bases
ATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGGGGAATGGTCGGCGGCGCGCTGGCGCTGGGTCTGGCGCAGCA
AGGGTTTACGGTGATGGTAATAGAACATGCCGCGCCTGCGCCGTTTGTGGCTAACAGCCAGCCTGACGTGCGGATTTCGG
CCATTAGCGCGGCATCCGTGGCGCTGCTCAAGGGACTGGGCGTTTGGGAGGCGGTGCAGGGGATGCGTAGCCATCCTTAT
CGGCGACTGGAGACCTGGGAGTGGGAAAACGCCCATGTGGTGTTCGACGCCGCTGAACTCAAGCTGCCGCTGTTAGGCTA
TATGGTGGAGAACAATGTCCTGCAACAGGCGCTATGGCAGGCGCTGGAAGCGCACCCCGGCGTGACGCTGCGCGTTCCGG
CTTCACTGTCGGCATTGCACCGCCGCCACGATGGTTATGCGCTGGAACTGGCCGACGGCGAATGGGTAACGCCGAAGTTA
GTGATTGGCGCCGACGGCGCGAATTCGCAGGTTCGGCAAATGGCGGGAATTGGCATTCATGCCTGGCAATATGCGCAGTC
GTGTATGCTTATCACCGTCAAGTGTGAAAATGCGCCAGGCGACAGCACCTGGCAGCAGTTTACGCCGACGGGGCCGCGCG
CCTTTTTGCCGCTGTTCGACGATTGGGCGTCGCTGGTGTGGTATGACGCTCCGGCACGTATTCGCCAGCTACAGAGTTTA
TCGATGACGCAATTGCAGGTGGAAATTAACCAGCATTTTCCGGCGCGTCTGGGGGCGGTGATGCCGGTCGCGGCGGGCGC
GTTTCCGCTAACCCGTCGTCACGCGTTGCAGTATGTACAACCGGGACTGGCGCTGGTAGGCGACGCGGCGCATACCATTC
ATCCGCTGGCCGGGCAGGGCGTCAACCTGGGGTATCGGGATGTTGATGCGTTGATTGATGTGCTGGCGAGTGCCCGTAGC
TATGGCGAGTCCTGGGCCAGCCATTCGGTGTTGAAACGCTATCAGACGCGGCGCATGGCAGATAATTTTATGATGCAAAG
TGGAATGGATCTGTTTTATGCCGGGTTCAGCAATGAACTGCCGCCGCTGCGTATTTTACGCAATATGGGGCTGATGGCGG
CGCAGCGCGCAGGCGTGCTAAAACGTCAGGCGCTGAAGTATGCGTTGGGGTTGTAG

Upstream 100 bases:

>100_bases
GTGTAATGCTTTGGCGCGGTTGTGACCAGTATGACTGACGTCAGCCCTAATGGGTAAAAAATCCTGTAAACTTGTCTAAA
ACGTAACAGGATGAATGACC

Downstream 100 bases:

>100_bases
TTTGTGTTATGCCGGGTGGCGGCAATACCTTACCCGGCCTGTATTCGCCCGTAGGCCTGATAAGCGTAGCGCCATCAGGC
AGTCCGAATTGCCAGAAAAG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVANSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPY
RRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLSALHRRHDGYALELADGEWVTPKL
VIGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARS
YGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL

Sequences:

>Translated_391_residues
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVANSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPY
RRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLSALHRRHDGYALELADGEWVTPKL
VIGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARS
YGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL
>Mature_390_residues
TNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVANSQPDVRISAISAASVALLKGLGVWEAVQGMRSHPYR
RLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQALEAHPGVTLRVPASLSALHRRHDGYALELADGEWVTPKLV
IGADGANSQVRQMAGIGIHAWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSLS
MTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLASARSY
GESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNELPPLRILRNMGLMAAQRAGVLKRQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=33.9378238341969, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI32967307, Length=347, Percent_Identity=33.7175792507205, Blast_Score=158, Evalue=9e-39,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=85.9335038363171, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=39.7959183673469, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=32.5268817204301, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=28.0742459396752, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321694, Length=288, Percent_Identity=28.4722222222222, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920760, Length=378, Percent_Identity=29.1005291005291, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42649; Mature: 42517

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVANSQPDVRISAISAASV
CCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCEEEEEHHHHHH
ALLKGLGVWEAVQGMRSHPYRRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQ
HHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
ALEAHPGVTLRVPASLSALHRRHDGYALELADGEWVTPKLVIGADGANSQVRQMAGIGIH
HHHCCCCEEEECCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
HHHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCEECCHHHHHHHHEEECCHHHHHHHHHC
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CCEEEEEEECCCCCHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLASARSYGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRILRNMGLMAAQRAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNQPTEIAIVGGGMVGGALALGLAQQGFTVMVIEHAAPAPFVANSQPDVRISAISAASV
CCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCEEEEEHHHHHH
ALLKGLGVWEAVQGMRSHPYRRLETWEWENAHVVFDAAELKLPLLGYMVENNVLQQALWQ
HHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
ALEAHPGVTLRVPASLSALHRRHDGYALELADGEWVTPKLVIGADGANSQVRQMAGIGIH
HHHCCCCEEEECCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLITVKCENAPGDSTWQQFTPTGPRAFLPLFDDWASLVWYDAPARIRQLQSL
HHHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCEECCHHHHHHHHEEECCHHHHHHHHHC
SMTQLQVEINQHFPARLGAVMPVAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CCEEEEEEECCCCCHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLASARSYGESWASHSVLKRYQTRRMADNFMMQSGMDLFYAGFSNEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRILRNMGLMAAQRAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]