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The map label for this gene is yeaJ [H]

Identifier: 194736073

GI number: 194736073

Start: 1324955

End: 1326448

Strand: Reverse

Name: yeaJ [H]

Synonym: SeSA_A1375

Alternate gene names: 194736073

Gene position: 1326448-1324955 (Counterclockwise)

Preceding gene: 194734254

Following gene: 194735594

Centisome position: 28.17

GC content: 45.31

Gene sequence:

>1494_bases
ATGAATTTGCATCATAAAGCGCTCAGGCACTTTATCTCGGCAAGCGTCATCGTTTTGACATCGTCCTTCCTGATTTACGA
ACTTATTGCCAGCGACAGGGCAATGAATGCCTATATGCGTTATATCATGGAGAGAGCCGATTCGTCGTTTTTGTACGATA
AGTACCAGAATCAAAGCATCGCCGCACATTTGATGCGAACTTTTGAAGCGCCGGGAGACCCCGTCACCGCAGAAAAACGC
CGCGCGTTTTGCGACGCCTTTGAGGCTATTAACGGTACGCACGGCGTCAACCTGACCCGGCATAATTATCCGGCGCTACA
TGGCACTCTGCAAACCGCCGCTACACAGTGTACCGATAATCTTGATGACGCCCTTTTACTGCCTGCGTTTGATCAGGCGG
TAAGCATCAACCGTTCGCAGGACGACCACAGTCACGGGCTGGGCACACTGGAGCTTAAATTCCGTTATTACGTTGATTTA
AATAAACATTATGTCTATTTCTATGATTTAATCAACTCACGGCGCTTCGCCATGCATCGTTGGACTTTTTTACAAAAAGG
CACAATGGGTATTAACAGAAAAGATATAGATAAACTTTTTACCGGCCGTACGGTTATTTCAAGTATTTACATGGATGATA
TTACCCAGGAAAACGTCATGAGCTTTTTAACGCCAGTCTATCTGGCGGGAACGTTAAAAGGTATCGTGATGGTGGATGTT
AACCAGGATAATTTAAAAAATATTTTTTATACCCAGGACCGTCCGCTGGTTTGGCGTTATCTTAACGTAACACTAAAGGA
TATGGACTCCGGAAAAGAAATTATTATTAATCAAAGCAAAAATAATCTGTTTCAATATGTGAATTATAGCCATGATATCC
CTGGTGGGCTGCGCGTTTCGTTGTCCCTTGATTTTACCTATTTCCTTGTCTCGTCCTGGAAGGCGCTGGCCTTTTACTTA
CTGGCAACGGCGCTCCTGCTTAATATGGTACGGATGCACTTTCGGCTTTATCGCAACGTCACACGCGAAAATATTAGCGA
TGCCATGACCGGGCTTTACAACCGTAAAATATTAACACCGGAGCTGGAACAGCGACTGCAACGCCTGGTCAATGCCGGGA
CGCCGGTGACATTTGTCGCTATTGATTGCGATAGGTTAAAACTGATCAACGATACCCAGGGGCACCAGGAAGGCGACCGA
ATTATTACCCTGTTGGCGAAAGCGATTAAAACATCGATTCGTAAAAGCGATTACGCCATTCGCCTCGGCGGCGATGAGTT
CTGTATTATTCTTGTTGATTACGCGGCGGATTTGGCTATCCATCTGCCGGAGCGTATTATTCGTAACCTGCAAATTATCG
CACCGGATAAGACAGTGCATTTTTCTGCCGGGATTTATAATATGCAGCCCAATGATACGATTAATGATGCCTACCAGGCT
TCCGATGCGCAGCTCTATCTGAACAAACAACAAAAACAACATCGTTCATCATAG

Upstream 100 bases:

>100_bases
TTCCATAACATCAACACGTTTTATTTTTAAACAGTCTATTAGAGGAATTCTCATCGCTATGGTAAACTAACGCATCCGAC
TTTCAGGGCTGTAGTTCATC

Downstream 100 bases:

>100_bases
TGCTTCGCAGTAAATGTTCATATTGCACCAGGAGTGAGAAATGGCCGAAATGACTGAAGTCGCGAAGGGTGCCGCTACAC
ACCAACGTTTAGTGGCTTTA

Product: ggdef domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNLHHKALRHFISASVIVLTSSFLIYELIASDRAMNAYMRYIMERADSSFLYDKYQNQSIAAHLMRTFEAPGDPVTAEKR
RAFCDAFEAINGTHGVNLTRHNYPALHGTLQTAATQCTDNLDDALLLPAFDQAVSINRSQDDHSHGLGTLELKFRYYVDL
NKHYVYFYDLINSRRFAMHRWTFLQKGTMGINRKDIDKLFTGRTVISSIYMDDITQENVMSFLTPVYLAGTLKGIVMVDV
NQDNLKNIFYTQDRPLVWRYLNVTLKDMDSGKEIIINQSKNNLFQYVNYSHDIPGGLRVSLSLDFTYFLVSSWKALAFYL
LATALLLNMVRMHFRLYRNVTRENISDAMTGLYNRKILTPELEQRLQRLVNAGTPVTFVAIDCDRLKLINDTQGHQEGDR
IITLLAKAIKTSIRKSDYAIRLGGDEFCIILVDYAADLAIHLPERIIRNLQIIAPDKTVHFSAGIYNMQPNDTINDAYQA
SDAQLYLNKQQKQHRSS

Sequences:

>Translated_497_residues
MNLHHKALRHFISASVIVLTSSFLIYELIASDRAMNAYMRYIMERADSSFLYDKYQNQSIAAHLMRTFEAPGDPVTAEKR
RAFCDAFEAINGTHGVNLTRHNYPALHGTLQTAATQCTDNLDDALLLPAFDQAVSINRSQDDHSHGLGTLELKFRYYVDL
NKHYVYFYDLINSRRFAMHRWTFLQKGTMGINRKDIDKLFTGRTVISSIYMDDITQENVMSFLTPVYLAGTLKGIVMVDV
NQDNLKNIFYTQDRPLVWRYLNVTLKDMDSGKEIIINQSKNNLFQYVNYSHDIPGGLRVSLSLDFTYFLVSSWKALAFYL
LATALLLNMVRMHFRLYRNVTRENISDAMTGLYNRKILTPELEQRLQRLVNAGTPVTFVAIDCDRLKLINDTQGHQEGDR
IITLLAKAIKTSIRKSDYAIRLGGDEFCIILVDYAADLAIHLPERIIRNLQIIAPDKTVHFSAGIYNMQPNDTINDAYQA
SDAQLYLNKQQKQHRSS
>Mature_497_residues
MNLHHKALRHFISASVIVLTSSFLIYELIASDRAMNAYMRYIMERADSSFLYDKYQNQSIAAHLMRTFEAPGDPVTAEKR
RAFCDAFEAINGTHGVNLTRHNYPALHGTLQTAATQCTDNLDDALLLPAFDQAVSINRSQDDHSHGLGTLELKFRYYVDL
NKHYVYFYDLINSRRFAMHRWTFLQKGTMGINRKDIDKLFTGRTVISSIYMDDITQENVMSFLTPVYLAGTLKGIVMVDV
NQDNLKNIFYTQDRPLVWRYLNVTLKDMDSGKEIIINQSKNNLFQYVNYSHDIPGGLRVSLSLDFTYFLVSSWKALAFYL
LATALLLNMVRMHFRLYRNVTRENISDAMTGLYNRKILTPELEQRLQRLVNAGTPVTFVAIDCDRLKLINDTQGHQEGDR
IITLLAKAIKTSIRKSDYAIRLGGDEFCIILVDYAADLAIHLPERIIRNLQIIAPDKTVHFSAGIYNMQPNDTINDAYQA
SDAQLYLNKQQKQHRSS

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081974, Length=496, Percent_Identity=68.5483870967742, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=30.1775147928994, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=219, Percent_Identity=29.6803652968037, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=30.0613496932515, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=30.9677419354839, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=31.8471337579618, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57048; Mature: 57048

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHHKALRHFISASVIVLTSSFLIYELIASDRAMNAYMRYIMERADSSFLYDKYQNQSI
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHH
AAHLMRTFEAPGDPVTAEKRRAFCDAFEAINGTHGVNLTRHNYPALHGTLQTAATQCTDN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
LDDALLLPAFDQAVSINRSQDDHSHGLGTLELKFRYYVDLNKHYVYFYDLINSRRFAMHR
CCCEEECCCCHHHHCCCCCCCCHHCCCEEEEEEEEEEEEECCCEEEEEEECCCCHHHHHH
WTFLQKGTMGINRKDIDKLFTGRTVISSIYMDDITQENVMSFLTPVYLAGTLKGIVMVDV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEC
NQDNLKNIFYTQDRPLVWRYLNVTLKDMDSGKEIIINQSKNNLFQYVNYSHDIPGGLRVS
CCCCCCCEEEECCCCEEEEEEEEEEECCCCCCEEEEECCCCCHHHHCCCCCCCCCCEEEE
LSLDFTYFLVSSWKALAFYLLATALLLNMVRMHFRLYRNVTRENISDAMTGLYNRKILTP
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ELEQRLQRLVNAGTPVTFVAIDCDRLKLINDTQGHQEGDRIITLLAKAIKTSIRKSDYAI
HHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
RLGGDEFCIILVDYAADLAIHLPERIIRNLQIIAPDKTVHFSAGIYNMQPNDTINDAYQA
EECCCCEEEEEEECHHHHHHHCHHHHHCCCEEECCCCEEEEECCEEECCCCCCCCHHHCC
SDAQLYLNKQQKQHRSS
CCHHEEEEHHHHHCCCC
>Mature Secondary Structure
MNLHHKALRHFISASVIVLTSSFLIYELIASDRAMNAYMRYIMERADSSFLYDKYQNQSI
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHH
AAHLMRTFEAPGDPVTAEKRRAFCDAFEAINGTHGVNLTRHNYPALHGTLQTAATQCTDN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
LDDALLLPAFDQAVSINRSQDDHSHGLGTLELKFRYYVDLNKHYVYFYDLINSRRFAMHR
CCCEEECCCCHHHHCCCCCCCCHHCCCEEEEEEEEEEEEECCCEEEEEEECCCCHHHHHH
WTFLQKGTMGINRKDIDKLFTGRTVISSIYMDDITQENVMSFLTPVYLAGTLKGIVMVDV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEC
NQDNLKNIFYTQDRPLVWRYLNVTLKDMDSGKEIIINQSKNNLFQYVNYSHDIPGGLRVS
CCCCCCCEEEECCCCEEEEEEEEEEECCCCCCEEEEECCCCCHHHHCCCCCCCCCCEEEE
LSLDFTYFLVSSWKALAFYLLATALLLNMVRMHFRLYRNVTRENISDAMTGLYNRKILTP
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ELEQRLQRLVNAGTPVTFVAIDCDRLKLINDTQGHQEGDRIITLLAKAIKTSIRKSDYAI
HHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
RLGGDEFCIILVDYAADLAIHLPERIIRNLQIIAPDKTVHFSAGIYNMQPNDTINDAYQA
EECCCCEEEEEEECHHHHHHHCHHHHHCCCEEECCCCEEEEECCEEECCCCCCCCHHHCC
SDAQLYLNKQQKQHRSS
CCHHEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]