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The map label for this gene is arsB [H]

Identifier: 194736038

GI number: 194736038

Start: 693578

End: 694867

Strand: Direct

Name: arsB [H]

Synonym: SeSA_A0717

Alternate gene names: 194736038

Gene position: 693578-694867 (Clockwise)

Preceding gene: 194734821

Following gene: 194735384

Centisome position: 14.73

GC content: 54.34

Gene sequence:

>1290_bases
ATGTTTTTGGCAGGGAGTATATTTTTACTGACGCTGGTACTGGTGATCTGGCAGCCCAGAGGCCTGAGTATTGGCTGGAG
CGCGAGTATCGGAGCTGCGCTGGCGCTGGGTACCGGAGTCATCCATATCGATGATATTCCCGTTGTCTGGAATATCGTCT
GGAACGCGACAGCGGCATTTATTGCGGTCATCATCATCAGCCTGCTGCTCGATGAGTCCGGCTTCTTTGAATGGGCCGCA
CTGCACGTCTCCCGCAGGGGTAACGGACGTGGCCGCCTTTTGTTTACCTGGATAGTCTTGCTTGGGGCCGCTGTTGCTGC
GTTGTTTGCCAATGACGGCGCCGCACTGATCCTGACGCCGATTGTAATTGCGATGCTGCTCGCACTGGGATTCAGCCAGG
GTACGACATTGGCCTTTGTCATGGCCGCAGGATTTATTGCAGATACGGCCAGCCTGCCGCTCATTGTTTCTAACCTGGTG
AATATCGTCTCGGCGGACTTTTTCGGTCTGAGCTTTACGCAGTATGCCTCCGTTATGATCCCCGTAGATGTGGTAGCGAT
TGCGGCCACGCTGGTCATGCTGCATCTCTTCTTCCGCAGGAACATTCCGGCGACATATGACGTTTCGCTGCTGAAAACGC
CTGCCAGTGTGATAAAGGATCCGGCAACGTTCAGGGCGGGCTGGATTGTTCTGTTATTGCTGCTTGTCGGTTTCTTTGTT
CTGGAGCCTCTTGGGATCCCGGTCAGTGCGATAGCGGCTACTGGCGCAGCAGTACTGTTTATAGTGGCGAAAAGAGGTCA
TACCATCAATACCGGAAAAGTCCTGCGCAGTGCGCCATGGCAGATCGTTATTTTTTCGCTGGGCATGTACCTGGTGGTCT
ATGGCCTGCGCAATGCAGGGCTCACGGAGTATCTGTCTGACGTGCTGAACCTGCTGGCAGTAAAGGGGTTATGGGCAGCA
ACGTTCGGCACCGGCCTCCTGACCGCGTTCCTGTCGTCGGTGATGAATAATATGCCGACGGTGTTGATTGGCGCGCTGTC
GATTGACGGGAGTACGGCGACTGGCATCGTCAAAGAGGCAATGATTTATGCCAACGTGATTGGCTGCGATTTAGGCCCGA
AAATCACCCCGATTGGCAGTCTGGCAACCCTGCTTTGGTTGCATGTGCTTGCCCAGAAAAATATAACGATTGCCTGGGGA
TATTACTTCCGCACCGGCATTATCATGACTCTGCCCGTGCTGTTTGTCACTTTGGCCGCGCTGGCGTTGCGGCTCTCCAT
CACTTTGTAA

Upstream 100 bases:

>100_bases
GCCTTCGGGAGGATGTGCGGGAATGGTTGGCAAAGTCGGCCTGCACCTCCTGTTGAGGCAGGAGAACATATTTATATAAT
CATATATAACGGAGTCTGAA

Downstream 100 bases:

>100_bases
TGAGATACAGATATGAGCAACATTACCATCTATCACAACCCTGCCTGCGGCACTTCTCGCAATACGCTGGAGATGATCCG
TAACAGCGGCAACGAACCGA

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MFLAGSIFLLTLVLVIWQPRGLSIGWSASIGAALALGTGVIHIDDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRRGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLSFTQYASVMIPVDVVAIAATLVMLHLFFRRNIPATYDVSLLKTPASVIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAATGAAVLFIVAKRGHTINTGKVLRSAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNLLAVKGLWAA
TFGTGLLTAFLSSVMNNMPTVLIGALSIDGSTATGIVKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLAQKNITIAWG
YYFRTGIIMTLPVLFVTLAALALRLSITL

Sequences:

>Translated_429_residues
MFLAGSIFLLTLVLVIWQPRGLSIGWSASIGAALALGTGVIHIDDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRRGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLSFTQYASVMIPVDVVAIAATLVMLHLFFRRNIPATYDVSLLKTPASVIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAATGAAVLFIVAKRGHTINTGKVLRSAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNLLAVKGLWAA
TFGTGLLTAFLSSVMNNMPTVLIGALSIDGSTATGIVKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLAQKNITIAWG
YYFRTGIIMTLPVLFVTLAALALRLSITL
>Mature_429_residues
MFLAGSIFLLTLVLVIWQPRGLSIGWSASIGAALALGTGVIHIDDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRRGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLSFTQYASVMIPVDVVAIAATLVMLHLFFRRNIPATYDVSLLKTPASVIKDPATFRAGWIVLLLLLVGFFV
LEPLGIPVSAIAATGAAVLFIVAKRGHTINTGKVLRSAPWQIVIFSLGMYLVVYGLRNAGLTEYLSDVLNLLAVKGLWAA
TFGTGLLTAFLSSVMNNMPTVLIGALSIDGSTATGIVKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLAQKNITIAWG
YYFRTGIIMTLPVLFVTLAALALRLSITL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=86.7132867132867, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45539; Mature: 45539

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLAGSIFLLTLVLVIWQPRGLSIGWSASIGAALALGTGVIHIDDIPVVWNIVWNATAAF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRRGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLSFTQYASVMI
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
PVDVVAIAATLVMLHLFFRRNIPATYDVSLLKTPASVIKDPATFRAGWIVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LEPLGIPVSAIAATGAAVLFIVAKRGHTINTGKVLRSAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSDVLNLLAVKGLWAATFGTGLLTAFLSSVMNNMPTVLIGALSIDGSTATGIVKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
MIYANVIGCDLGPKITPIGSLATLLWLHVLAQKNITIAWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LALRLSITL
HHHHHHCCC
>Mature Secondary Structure
MFLAGSIFLLTLVLVIWQPRGLSIGWSASIGAALALGTGVIHIDDIPVVWNIVWNATAAF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRRGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSQGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLSFTQYASVMI
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
PVDVVAIAATLVMLHLFFRRNIPATYDVSLLKTPASVIKDPATFRAGWIVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
LEPLGIPVSAIAATGAAVLFIVAKRGHTINTGKVLRSAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSDVLNLLAVKGLWAATFGTGLLTAFLSSVMNNMPTVLIGALSIDGSTATGIVKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHH
MIYANVIGCDLGPKITPIGSLATLLWLHVLAQKNITIAWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LALRLSITL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]