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The map label for this gene is iaaA

Identifier: 194735946

GI number: 194735946

Start: 970284

End: 971225

Strand: Direct

Name: iaaA

Synonym: SeSA_A0998

Alternate gene names: 194735946

Gene position: 970284-971225 (Clockwise)

Preceding gene: 194734482

Following gene: 194734810

Centisome position: 20.6

GC content: 59.98

Gene sequence:

>942_bases
ATGAATAAAGCAGTGATTGCGATTCACGGCGGCGCAGGCGCGATTGCTCGCGCGCAAATGAGCCATGAGCAGGAGCTACG
CTATATCCAGGCGCTGTCAGAGATTGTCGAAAGCGGGCAAAAGATGCTGGAAGCAGGTGACAGCGCGCTTGATGTAGTGA
CGGAAGCGGTGCGCCTGCTGGAAGCGTGTCCGCTCTTCAATGCGGGTATCGGCGCGGTCTATACCCGAGACGGGACTCAT
GAACTGGATGCCTGCGTGATGGACGGCAATACCCTGAAAGCCGGAGCCGTCGCGGGCGTGAGTCATGTTCGCCATCCTGT
GCTCGCCGCCAGACTGGTGATGGAGCGCAGCCCGCATGTACTCATGGTGGGGGAAGGGGCGGAAAACTTTGCCTTTTCAC
AGGGGATGGCGCGCGTTTCGCCGGATATCTTTTCCACCCCGGCGCGTTACGAGCAATTGCTGGCGGCGCGTGCGGCGGGC
GAAATGGCGCTCGATCACAGCGGCGCGCCGCTGGACGAAACGAAAAAAATGGGTACAGTCGGCGCGGTGGCGCGGGATAA
ATTCGGCAATCTGGCGGCGGCAACGTCTACCGGTGGGATGACCAATAAATTGCCCGGGCGCGTCGGCGACAGCCCGCTGG
TCGGCGCAGGGTGTTATGCCAACAACGCCAGCGTCGCCGTTTCCTGTACCGGAACGGGAGAGGTCTTTATTCGCACGCTC
GCGGCTTACGATATCGCCGCATTGATGGAATATGGCGGACTCAGCCTTGCCGATGCCTGTGAGCGCGTGGTGATGGAAAA
ATTACCGGCGCTGGGCGGCAGCGGTGGATTAATCGCCGTTGATCACGAAGGTAATGTCGCGTTGCCGTTTAACAGCGAAG
GGATGTATCGCGCCTGGGGATATGCCGGGGATACCCCTACCACAGGAATTTACCGGGAATAA

Upstream 100 bases:

>100_bases
ACCTTACGCATCTTTCTATATTCAAAAATCGAATGAATAACTCACAACAATTCTGATAGTTATAGTAAAAGTTGTGTGCC
ACCGTTAATGGACCCTAAAA

Downstream 100 bases:

>100_bases
GGAGCCGCAGGTGCCGCACAGCGATGAACTGGATAGCCGCGATGTTCTGTCCGTCAGTGGCCTGAATATCGCGTTTCACC
ACGAAGGGCAACAGATAGAC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNKAVIAIHGGAGAIARAQMSHEQELRYIQALSEIVESGQKMLEAGDSALDVVTEAVRLLEACPLFNAGIGAVYTRDGTH
ELDACVMDGNTLKAGAVAGVSHVRHPVLAARLVMERSPHVLMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAG
EMALDHSGAPLDETKKMGTVGAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRTL
AAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLIAVDHEGNVALPFNSEGMYRAWGYAGDTPTTGIYRE

Sequences:

>Translated_313_residues
MNKAVIAIHGGAGAIARAQMSHEQELRYIQALSEIVESGQKMLEAGDSALDVVTEAVRLLEACPLFNAGIGAVYTRDGTH
ELDACVMDGNTLKAGAVAGVSHVRHPVLAARLVMERSPHVLMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAG
EMALDHSGAPLDETKKMGTVGAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRTL
AAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLIAVDHEGNVALPFNSEGMYRAWGYAGDTPTTGIYRE
>Mature_313_residues
MNKAVIAIHGGAGAIARAQMSHEQELRYIQALSEIVESGQKMLEAGDSALDVVTEAVRLLEACPLFNAGIGAVYTRDGTH
ELDACVMDGNTLKAGAVAGVSHVRHPVLAARLVMERSPHVLMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAG
EMALDHSGAPLDETKKMGTVGAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRTL
AAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLIAVDHEGNVALPFNSEGMYRAWGYAGDTPTTGIYRE

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family

Homologues:

Organism=Homo sapiens, GI145275202, Length=305, Percent_Identity=38.6885245901639, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI145275200, Length=305, Percent_Identity=38.6885245901639, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI98991769, Length=275, Percent_Identity=33.0909090909091, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI285002251, Length=255, Percent_Identity=30.5882352941176, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=88.1789137380192, Blast_Score=564, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17569239, Length=251, Percent_Identity=29.4820717131474, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32564649, Length=316, Percent_Identity=26.2658227848101, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=36.7601246105919, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=32.4626865671642, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652135, Length=236, Percent_Identity=32.6271186440678, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330102, Length=236, Percent_Identity=32.6271186440678, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IAAA_SALTY (Q7CQV5)

Other databases:

- EMBL:   AE006468
- RefSeq:   NP_459824.1
- HSSP:   P37595
- ProteinModelPortal:   Q7CQV5
- SMR:   Q7CQV5
- MEROPS:   T02.002
- PRIDE:   Q7CQV5
- GeneID:   1252366
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0847
- HOGENOM:   HBG735787
- OMA:   NKQVGRV
- ProtClustDB:   PRK10226
- BioCyc:   STYP99287:STM0847-MONOMER
- InterPro:   IPR000246
- PANTHER:   PTHR10188

Pfam domain/function: PF01112 Asparaginase_2

EC number: =3.4.19.5

Molecular weight: Translated: 32473; Mature: 32473

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAVIAIHGGAGAIARAQMSHEQELRYIQALSEIVESGQKMLEAGDSALDVVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EACPLFNAGIGAVYTRDGTHELDACVMDGNTLKAGAVAGVSHVRHPVLAARLVMERSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAGEMALDHSGAPLDETKKMGTV
EEECCCCCHHHHHCCHHHCCCHHHCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHCCCH
GAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRTL
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLIAVDHEGNVALPFNSEGMYRAWG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYRE
CCCCCCCCCCCCC
>Mature Secondary Structure
MNKAVIAIHGGAGAIARAQMSHEQELRYIQALSEIVESGQKMLEAGDSALDVVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EACPLFNAGIGAVYTRDGTHELDACVMDGNTLKAGAVAGVSHVRHPVLAARLVMERSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAGEMALDHSGAPLDETKKMGTV
EEECCCCCHHHHHCCHHHCCCHHHCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHCCCH
GAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRTL
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLIAVDHEGNVALPFNSEGMYRAWG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYRE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609