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The map label for this gene is glgX [H]

Identifier: 194735910

GI number: 194735910

Start: 1604606

End: 1606666

Strand: Reverse

Name: glgX [H]

Synonym: SeSA_A1670

Alternate gene names: 194735910

Gene position: 1606666-1604606 (Counterclockwise)

Preceding gene: 194735407

Following gene: 194734894

Centisome position: 34.12

GC content: 52.3

Gene sequence:

>2061_bases
ATGACAACAAACAGGGCTTTTGAAATCAGATCAGGATACAGCCATACGCTTGGCGCGAATTATGACGGCGAAGGCGTGAA
TTTCGCTATTTTTTCCGCCCATGCGGAACGCGTCGATCCGAGCGGGGAGCATGAAATCGCTCGTCTGGAGTTACCGGAGT
ATACCGACGAAATATGGCACGGCTGTGTCCCGAAGTTGCAGCCCGGCGCGCTGTATGGGTACCGTGTTTACGGTCCTTAT
GACCCGGAAAATGGTCATCGTTTTAACCCCAATAAGCTGCTTATTGACCCTTATGCCCGTGAGCTGGTGGGCGATATTCA
GTGGAACGATGCGCATTTCGCGTATCAATTACTTCATGACGATAAAGATTTGACCTTTGATGACCAGGATAGCGCGCCAT
TTACGCCTAAATGCCGGGTGATTGATCCGGCTGAGGCGAATTGGGAGGATAGACAACGGCCTTCCATACCGTGGTCGAAC
GCCATTATTTATGAAACGCATGTAAAAGGCTTTACCCAGCTTAATTCCGCGATCCCTGAGCCTATACGCGGTACGTTTGA
GGGCATGGGGCATAAAGCCAGCGTGGATTACATTAAAAGTCTGGGGATTACCTCCGTCGAGCTTTTACCCGTTCACTGGT
TCCCGGACGATCAGCATCTGCTCGATAAAGGACTGAAAAATTTCTGGGGATACAACTCGCTGGGCTTTTTTGCGCCGGCG
ACGCGCTACTATGGTCCAAAAGGTATTCAGGGATTCCGCGATATGGTGCGCGCTTTCCATGATGCCGGCATCGAAGTGAT
ACTGGATGTGGTCTATAACCATACGGCAGAAGGGAATGAGCTTGGTCCCACGCTGTCATTTAAAGGTATCGATAACTTCT
CTTACTATCGCACCATGCCCGATCAGCATCGCTATTACATTAACGATACAGGGACAGGTAATACCGTCAATACCTCTCAT
CCACGGGTACTGCAAATGGTGATGGATTCGCTTCGCTATTGGGCGGAGTCAATGCATGTCGACGGCTTTCGTTTTGATTT
GGGCACCATTCTTGGTCGTGAGCCGGAAGGCTTTGATCAGAGAGGCGGCTTTTTTGATGCGGTAACGCAAGACCCGGTAC
TGGCAAAACTGAAACTGATTGGCGAACCCTGGGATATCGGACCGGGCGGCTATCAGGTTGGCGGTTTTCCACCAGGGTGG
GGCGAATGGAATGATAAATACCGCGATACGGTGCGCGAATACTGGAAAGGTGACAACGTCACAAATGATTTCGCTGCACG
CCTGCTGGGTTCCGGCGATCTCTATGATTTGCGCGGCCGTCGCCCCTGGTCAAGCGTTAACTTTATTACTGCCCATGACG
GCTTCACGCTGAACGATCTGGTGTCTTATAACGACAAACATAATGAAGCCAATGGCGAAGATAATAATGACGGGCATAAT
GATAACCGCTCCTGTAACTATGGCGCAGAAGGGCCAACCGACGACGAGGGCATCAATGCGATACGCGAGCAACAGAAACG
TAATTTCCTGACCACCTTGCTTTTCTCGCACGGCACGCCGATGTTATTAGCGGGCGATGAGTTTGGCCGCAGTCAGATGG
GCAATAACAATGGCTACTGTCAGGACAGTGAAATATCGTGGGTCCACTGGGATAATTTGCCGGAAACCGCCAATGCGCTA
CGGGAGTTTACCCGTCATCTGATTCAACTGCGCGCCACGCAACCGTTGCTGCGCCGGGAGAGCTGGCGTGACGGTCTGGA
GATCCGCTGGTTTAACGCCGGCGGAGGGGCGCAGCAATCCGAACAATGGGATGAGGGATCGACGATAGGCGTATGTATTA
GTCGACCCGATCTTCAGCCGGAAGCGGGGATCTGGCACGACGCCTTACTGCTTTTCAATCCGTTTGAAGGCAGCGTACCG
TTCCGTATTCCGATGTGGGGAGAAGGCGGCTGGGTACTTGAACTGACGACTGCCGATAACGCGCAGCAGGGAATGCGGAT
TACCGAAGAAATGGATTTCGATCTGGCCGGGCGCAGCATCGTGTTATTCAGACGCCCCTGA

Upstream 100 bases:

>100_bases
TGGCCTCACATCAGGGCGGCGTACTGGTTTTAATGAGCGACTGAGTTTATGTGTTGATGTGCCGTGACAGGACAACGTTT
ACCGTGGGTGGAGAAAGAGA

Downstream 100 bases:

>100_bases
TAGTGAAACCGCGCTCCGGACCCAACCGGCCTTGATGGCCGGTTTTTTTACTGGCGCTCAGGCGATATCATGCCGCAGAA
ATAACACCGTTGTCAGTTTA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVDPSGEHEIARLELPEYTDEIWHGCVPKLQPGALYGYRVYGPY
DPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSN
AIIYETHVKGFTQLNSAIPEPIRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGFFAPA
TRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNTVNTSH
PRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGW
GEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNNDGHN
DNRSCNYGAEGPTDDEGINAIREQQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPETANAL
REFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVP
FRIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP

Sequences:

>Translated_686_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVDPSGEHEIARLELPEYTDEIWHGCVPKLQPGALYGYRVYGPY
DPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSN
AIIYETHVKGFTQLNSAIPEPIRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGFFAPA
TRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNTVNTSH
PRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGW
GEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNNDGHN
DNRSCNYGAEGPTDDEGINAIREQQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPETANAL
REFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVP
FRIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP
>Mature_685_residues
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVDPSGEHEIARLELPEYTDEIWHGCVPKLQPGALYGYRVYGPYD
PENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSNA
IIYETHVKGFTQLNSAIPEPIRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGFFAPAT
RYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNTVNTSHP
RVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWG
EWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNNDGHND
NRSCNYGAEGPTDDEGINAIREQQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPETANALR
EFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVPF
RIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=610, Percent_Identity=47.7049180327869, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77816; Mature: 77685

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVDPSGEHEIARLELPEYTDEIWH
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCEEEEECCHHHHHHHH
GCVPKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHD
CCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCCCCCHHEEEEEEEC
DKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSNAIIYETHVKGFTQLNSAIPE
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCC
PIRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGFFAPA
CHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCH
TRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMP
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHEECCC
DQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQ
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCC
RGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYWKGDNV
CCCEECCCCCCCCEEHHEECCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
TNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNNDGHN
CHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCC
DNRSCNYGAEGPTDDEGINAIREQQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCCCCCCC
QDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCC
EQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVPFRIPMWGEGGWVLELTTADN
CCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCC
AQQGMRITEEMDFDLAGRSIVLFRRP
CHHCCEEHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVDPSGEHEIARLELPEYTDEIWH
CCCCEEEECCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCEEEEECCHHHHHHHH
GCVPKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHD
CCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCCCCCHHEEEEEEEC
DKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSNAIIYETHVKGFTQLNSAIPE
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCC
PIRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGFFAPA
CHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCH
TRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMP
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHEECCC
DQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQ
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCC
RGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYWKGDNV
CCCEECCCCCCCCEEHHEECCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
TNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNNDGHN
CHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCC
DNRSCNYGAEGPTDDEGINAIREQQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCCCCCCC
QDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCC
EQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVPFRIPMWGEGGWVLELTTADN
CCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCC
AQQGMRITEEMDFDLAGRSIVLFRRP
CHHCCEEHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]