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The map label for this gene is tusD

Identifier: 194735809

GI number: 194735809

Start: 3501399

End: 3501785

Strand: Reverse

Name: tusD

Synonym: SeSA_A3647

Alternate gene names: 194735809

Gene position: 3501785-3501399 (Counterclockwise)

Preceding gene: 194735277

Following gene: 194736554

Centisome position: 74.36

GC content: 57.88

Gene sequence:

>387_bases
ATGCGTTATGCCATTATGGTCACCGGGCCGGCATATGGCACCCAGCAGGCCAGCAGCGCTTTGCAGTTCGCCCACGCGCT
GCTCAATGAGGGGCATGAGTTAGCCAGCGTCTTTTTCTATCGGGAAGGTGTCTATAACGCCAATCTCCTGACCTCGCCGG
CGAGCGATGAATATGATCTGGTACGCGCCTGGCAGAAATTGAATACGCAGCATGGCGTAGCGCTGAACATCTGTGTCGCG
GCGGCGCTTCGTCGCGGTATTATTGATGAAACTGAAGCTGGCAGACTGGCGTTGCCGTCCGCCAATCTTCAGCCGGGCTT
TACGCTAAGCGGACTGGGCGCGCTGGCGGAGGCATCTCTCACCTGCGATCGGGTGGTGCAGTTTTAA

Upstream 100 bases:

>100_bases
CCATTAACCAGGTAGCCGATCGTCTGAATATCTCAAAACACACGGTTTACCTCTATATTCGCCAGTTCAAAAGCGGCGAT
TTTCAGGGGCAGGATAAATA

Downstream 100 bases:

>100_bases
TGAAACGTATTGCATTTGTCTTTTCCACCGCGCCGCACGGCAGCGCCTCAGGCCGTGAAGGACTGGATGCGCTGCTGGCG
ACGTCAGCTCTAACGGAAGC

Product: sulfur transfer complex subunit TusD

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MRYAIMVTGPAYGTQQASSALQFAHALLNEGHELASVFFYREGVYNANLLTSPASDEYDLVRAWQKLNTQHGVALNICVA
AALRRGIIDETEAGRLALPSANLQPGFTLSGLGALAEASLTCDRVVQF

Sequences:

>Translated_128_residues
MRYAIMVTGPAYGTQQASSALQFAHALLNEGHELASVFFYREGVYNANLLTSPASDEYDLVRAWQKLNTQHGVALNICVA
AALRRGIIDETEAGRLALPSANLQPGFTLSGLGALAEASLTCDRVVQF
>Mature_128_residues
MRYAIMVTGPAYGTQQASSALQFAHALLNEGHELASVFFYREGVYNANLLTSPASDEYDLVRAWQKLNTQHGVALNICVA
AALRRGIIDETEAGRLALPSANLQPGFTLSGLGALAEASLTCDRVVQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family

Homologues:

Organism=Escherichia coli, GI1789743, Length=128, Percent_Identity=85.9375, Blast_Score=232, Evalue=6e-63,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): TUSD_SALA4 (B5F8G3)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002148370.1
- ProteinModelPortal:   B5F8G3
- SMR:   B5F8G3
- GeneID:   6793576
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B3648
- HOGENOM:   HBG538485
- OMA:   QDGVYNS
- ProtClustDB:   PRK00207
- GO:   GO:0005737
- HAMAP:   MF_00390
- InterPro:   IPR003787
- InterPro:   IPR017463
- TIGRFAMs:   TIGR03012

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 13758; Mature: 13758

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYAIMVTGPAYGTQQASSALQFAHALLNEGHELASVFFYREGVYNANLLTSPASDEYDL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEECCCCCCCHHH
VRAWQKLNTQHGVALNICVAAALRRGIIDETEAGRLALPSANLQPGFTLSGLGALAEASL
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
TCDRVVQF
CHHHHHCC
>Mature Secondary Structure
MRYAIMVTGPAYGTQQASSALQFAHALLNEGHELASVFFYREGVYNANLLTSPASDEYDL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEECCCCCCCHHH
VRAWQKLNTQHGVALNICVAAALRRGIIDETEAGRLALPSANLQPGFTLSGLGALAEASL
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
TCDRVVQF
CHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA