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The map label for this gene is nadB [H]

Identifier: 194735715

GI number: 194735715

Start: 2733043

End: 2734665

Strand: Direct

Name: nadB [H]

Synonym: SeSA_A2834

Alternate gene names: 194735715

Gene position: 2733043-2734665 (Clockwise)

Preceding gene: 194736667

Following gene: 194736836

Centisome position: 58.04

GC content: 55.39

Gene sequence:

>1623_bases
ATGATGACAACTCCTGAACTGTCCTGTGATGTGTTAATTATCGGCAGCGGCGCGGCCGGACTCTCGCTGGCGCTGCGTCT
GGCTGAAAAACATAAGGTTATCGTCCTGAGTAAAGGGCCGGTGAGCGAGGGCTCCACGTTTTATGCGCAGGGCGGTATTG
CCGCCGTGTTTGATGAAACCGACAGTATTGCGTCGCATGTCGAAGATACGCTGATTGCCGGCGCCGGAATTTGCGATCGC
CACGCGGTGGAGTTTGTCGCCAGCAACGCTCGTACCTGCGTGCAGTGGCTTATCGATCAGGGCGTGCTGTTTGATACGCA
TGTCCAGCCAAACGGCAAGGAGAGCTATCATCTCACGCGTGAAGGCGGCCATAGCCATCGCCGTATCCTTCATGCCGCTG
ACGCTACCGGAAAGGAAGTCGAAACCACGTTGGTCAGTCGGGCGCAAAATCATCCTAATATTCAGGTGCTGGAACGCAGC
AACGCCGTTGATTTAATTATCTCCGATAAAATGGGTCTGCCAGGGCCTCGCCGGGTCGTCGGCGCCTGGATCTGGAATCG
CAATAAAGAGTGGGTTGAAACCTGTCATGCGAAGTCGGTGGTGCTGGCGACCGGCGGCGCCTCAAAGGTTTATCAATACA
CCACTAACCCGGATATTTCCTCCGGCGACGGTATCGCGATGGCCTGGCGCGCAGGCTGTCGGGTCGCTAATCTGGAATTT
AACCAGTTTCATCCCACCGCCCTTTATCACCCGCAGGCGCGTAATTTTCTCCTTACCGAAGCGCTGCGCGGCGAAGGCGC
ATACCTTAAACGTCCGGACGGTTCACGGTTTATGCCGGACGTTGACGAACGCGGCGAACTGGCGCCGCGCGATATTGTCG
CCCGCGCTATTGACCATGAGATGAAACGACTCGGCGCAGACTGTATGTTTCTCGATATCAGCCACAAGCCTGACGATTTT
GTCCGCCAGCACTTCCCGATGATCTATGCGAAACTGCTCGATTTGGGGATGGATTTAACCAAAGAACCGATTCCCGTTGT
TCCTGCCGCGCACTATACCTGCGGCGGCGTGGTTGTCGATGATTATGGTCGTACCGATGTAGACGGCTTATATGCCATTG
GCGAAGTCAGTTACACCGGCCTGCACGGCGCTAACCGTATGGCGTCCAACTCGTTGCTGGAATGCTTAGTGTACGGCTGG
TCCGCAGCAATGGATATCGACAGGCGAATGCCTTCTGTCCACAGCGTTGACGTCCTTCCCGCCTGGGATGAAAGCCGCGT
GGAGAACGCCGATGAGCGGGTGGTGATTCAGCATAACTGGCATGAACTGCGCCTGCTGATGTGGGACTATGTGGGCATTG
TGCGCACGACGAAACGGCTGGAGCGCGCCCTACGGCGTATTACTATGTTGCAACAAGAGATTGACGAATATTACGCCAAC
TTTCGCGTCTCGAATAATTTACTGGAGCTGCGCAATCTGGTGCAAGTGGCCGAACTGATTGTGCGTTGCGCGATGATGCG
TAAAGAGAGCCGGGGGCTGCACTTTACGCTGGATTATCCGCAACAACTGGCTGAATCAGGCCCATCGATCCTTTCGCCGT
TAACGCCTCACATAAACAGATAA

Upstream 100 bases:

>100_bases
TATGTTTAATAAGCACAACACCAGCGAAATCATCACGTGCTTTTTAGCGCCATATAGTGCTAATCTGCCGCAACCGTGTT
TAGTAAATTAAACAAAAACC

Downstream 100 bases:

>100_bases
AATGCCTGGGTCAGCGCCGTATAACTTTCAGAATAATGCTGGTCTGACCCGCGAATTACTAATCTGTCGCTAAAGCACTC
TCCGGCTTGCGGGGAGAATG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Sequences:

>Translated_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
>Mature_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=31.2741312741313, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1788928, Length=540, Percent_Identity=90, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=529, Percent_Identity=32.3251417769376, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1786942, Length=569, Percent_Identity=30.5799648506151, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17550100, Length=539, Percent_Identity=30.6122448979592, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=29.7397769516729, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71986328, Length=459, Percent_Identity=27.4509803921569, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=31.1151079136691, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=30.5206463195691, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.734693877551, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.6376811594203, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663005, Length=558, Percent_Identity=30.1075268817204, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI17137288, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24655642, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24655647, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60166; Mature: 60166

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKMGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHEE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDVLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKMGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHEE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDVLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]