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The map label for this gene is miaE [H]

Identifier: 194735698

GI number: 194735698

Start: 4589364

End: 4590176

Strand: Direct

Name: miaE [H]

Synonym: SeSA_A4726

Alternate gene names: 194735698

Gene position: 4589364-4590176 (Clockwise)

Preceding gene: 194734898

Following gene: 194734516

Centisome position: 97.46

GC content: 54.98

Gene sequence:

>813_bases
ATGACGGTACGGCAGCGTCTGCTGTCGTATTTTTTTAACAGGTTACGTATGAATTACCCGCAAATACTCTCTCCGGTGCT
TAATTTCCTGCATTGCCCGACGCCGCAGGCGTGGATTGTCCAGGCCCGCGATCCGCAAAATCTTCCTCTTCTGCTTACCG
ATCACTTGATCTGCGAACTGAAGGCTGCGCAAACCGCGTTGCTGTTGGTGCGTAAATATGTCGCCGATAAATCGGGCGCC
GACGCGCTGCTCGCCTGGCTGCAACCTTACGAGGCGTTCGCGTTTCGTCAGGGGCCGGAGCCGGATTTTGTTGCCCTGCA
TAGACAGATTAGCAAAAGCGCCATGCCGCAAACTGATGACCCCTGGGGGCGTCAGCTGATCGACCGTATGGTGTTATTGA
TTAAAGAGGAGTTGCACCATTTTTGGCAGGTACGCGAGGTCATGCAGGCCAGAAACATTCCTTATGTGAAAATCACCGCC
AGCCGCTACGCGAAAGGAATGTTGAAAGCGGTACGCACGCACGAACCGCTCACCTTAATTGATAAGCTGATTTGTGGGGC
GTATATCGAAGCCCGCTCCTGCGAACGTTTTGCCGCTCTGGCGCCCTGGCTGGATGAGGATTTACAGACGTTTTATCTCT
CGCTTCTGCGCTCCGAAGCGCGGCATTATCAGGATTACCTGGCGCTGGCGCAGCAGATTTCCGCCGAGGACATTTCTGCG
CGCGTGCGCTATTTTGGCGACGTGGAAGCTGACCTTATCCTGTCGCCAGACCGCGAGTTTCGTTTTCATAGCGGCGTGCC
AGCCGCCGGATAA

Upstream 100 bases:

>100_bases
GCTGGGGAACCTATTTTGAAGACCCTAATGGCGAAGAGGGTGACGACGACGATTATGTCGATGAAGATGACGACGGCGTG
CGTCACTAAAGCTGTATAGT

Downstream 100 bases:

>100_bases
AGGGTAAAATTAAAGATTAGCGTTTTTATCTTTTACGCTGTCGCCGTCTGCAAACGTTGTGGTGGTCATTCCGCCCGGCA
TCAAGCTGCTGGCGAGCAGG

Product: tRNA-(ms[2]io[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGA
DALLAWLQPYEAFAFRQGPEPDFVALHRQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITA
SRYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISA
RVRYFGDVEADLILSPDREFRFHSGVPAAG

Sequences:

>Translated_270_residues
MTVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGA
DALLAWLQPYEAFAFRQGPEPDFVALHRQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITA
SRYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISA
RVRYFGDVEADLILSPDREFRFHSGVPAAG
>Mature_269_residues
TVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGAD
ALLAWLQPYEAFAFRQGPEPDFVALHRQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITAS
RYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISAR
VRYFGDVEADLILSPDREFRFHSGVPAAG

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 31138; Mature: 31007

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICEL
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
KAAQTALLLVRKYVADKSGADALLAWLQPYEAFAFRQGPEPDFVALHRQISKSAMPQTDD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
PWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
DKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVRYFGDVEADLILSPDREFRFHSGVPAAG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICEL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
KAAQTALLLVRKYVADKSGADALLAWLQPYEAFAFRQGPEPDFVALHRQISKSAMPQTDD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
PWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
DKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVRYFGDVEADLILSPDREFRFHSGVPAAG
HHHHHCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]