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The map label for this gene is intS [H]

Identifier: 194735670

GI number: 194735670

Start: 650260

End: 651447

Strand: Direct

Name: intS [H]

Synonym: SeSA_A0666

Alternate gene names: 194735670

Gene position: 650260-651447 (Clockwise)

Preceding gene: 194735171

Following gene: 194734149

Centisome position: 13.81

GC content: 46.46

Gene sequence:

>1188_bases
ATGAAGCTAACAGCCCGCCAGATCAGTACGGCGAAACCAACGGAGAAACCATATAAGCTGTCTGATGGCGGTGGTTTGTA
TCTGCTGGTTAACCCGAACGGCTCCCGATACTGGCGTATGAAGTATCGCTATGCAGGTAAGGAGAAACTTCTTTCCATTG
GCGTTTATCCTGATGTGACGCTTGCGGAAGCAAGAGACAAACGAACCCAGGCTAAACGTATACTTGCTGCGGGCGATGAT
CCATCTGAAGTAAAACAGGCCGAGAGAGAGGCAAAGAATCTTGCGGTTAATAACAGCTTTGAACTTCTGGCGCTTGAATG
GCATGAGCATAAAAAGCCAAACTGGTCTTCAGGCTATGCTGACGACATTCTTGAATATCTGCGAAAAGACATATTCCCAT
ATATCGGTAAGAAAGCCATAACAGATATTAAGCCGATGACTATGCTCTCCGTGCTGAAAAAAATGGAGGATCGTGGTGTA
CTGGATAAGCTAAAAAAAACTAGGCAAGCCTGCCGACAGATATTTACTTATGCAATTATAACCGGCAGAGCTGAGTTTAA
TCCCGTAACGGATTTAGCTGGAGCGCTGAAAACGCCAAAGCAACAACATTTCCCGCATCTGATGCCAACTCAGATTGGCC
CCTTCATTCATGCTGTTAATACCTACTCAGGCAGTAAAGTAACCCGCATCGCTACGCTATTACTTATGTATACCAGTGTC
AGGACAATCGAGTTAAGGGCTTCTGAATGGACTGAGTTCGACCTGGACAACGATTTGTGGCAGATCCCCAAGGAACGTAT
GAAGATGCGCCGTCCTCACCTTGTACCGCTTTCAAGGCAGGTTAAATCTCATCTGCTGGAACTAAAAAAGATCACTGGTT
GGGGTAAGTATGTTTTCCCCGGTCGCAATGATGCCCACAAGCCAATGAGTGAGGCCAGTATTAATCAGGTGATTAAGCGC
ATAGGTTTTGCAGGAAAGGTCACAGGACACGGATTCAGACACACCATGAGTACCATATTACATGAAAAGGGGTTCAACTC
TGCATGGATAGAAGCACAACTGGCGCACGCTGACAGGAATACTATCCGTGGAACTTACAATCATGCTCAGTACCTTGACG
GTCGACGGGAGATGCTTCAGTGGTACGCTGACTATCTGGATGAGTTGGTTGGAAAAGTGGTGGGTTAA

Upstream 100 bases:

>100_bases
TTCATTATCTCAAAGTCTCTTATCGTCTATTGAAATCTACGTTCATCTGGGGGTACATTCGGGGGTATATTCAGGTTCAA
TGAAGTGAGATACCCCCAAT

Downstream 100 bases:

>100_bases
CCTGTTTTCTTCATGTGTGAGGAAGAGTAGTTGTTCCACTTGTTCAAGTTGTTCCAGATATATAGATATGAAGGTAACTT
ACTGTTTTTATTTATATATG

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKLTARQISTAKPTEKPYKLSDGGGLYLLVNPNGSRYWRMKYRYAGKEKLLSIGVYPDVTLAEARDKRTQAKRILAAGDD
PSEVKQAEREAKNLAVNNSFELLALEWHEHKKPNWSSGYADDILEYLRKDIFPYIGKKAITDIKPMTMLSVLKKMEDRGV
LDKLKKTRQACRQIFTYAIITGRAEFNPVTDLAGALKTPKQQHFPHLMPTQIGPFIHAVNTYSGSKVTRIATLLLMYTSV
RTIELRASEWTEFDLDNDLWQIPKERMKMRRPHLVPLSRQVKSHLLELKKITGWGKYVFPGRNDAHKPMSEASINQVIKR
IGFAGKVTGHGFRHTMSTILHEKGFNSAWIEAQLAHADRNTIRGTYNHAQYLDGRREMLQWYADYLDELVGKVVG

Sequences:

>Translated_395_residues
MKLTARQISTAKPTEKPYKLSDGGGLYLLVNPNGSRYWRMKYRYAGKEKLLSIGVYPDVTLAEARDKRTQAKRILAAGDD
PSEVKQAEREAKNLAVNNSFELLALEWHEHKKPNWSSGYADDILEYLRKDIFPYIGKKAITDIKPMTMLSVLKKMEDRGV
LDKLKKTRQACRQIFTYAIITGRAEFNPVTDLAGALKTPKQQHFPHLMPTQIGPFIHAVNTYSGSKVTRIATLLLMYTSV
RTIELRASEWTEFDLDNDLWQIPKERMKMRRPHLVPLSRQVKSHLLELKKITGWGKYVFPGRNDAHKPMSEASINQVIKR
IGFAGKVTGHGFRHTMSTILHEKGFNSAWIEAQLAHADRNTIRGTYNHAQYLDGRREMLQWYADYLDELVGKVVG
>Mature_395_residues
MKLTARQISTAKPTEKPYKLSDGGGLYLLVNPNGSRYWRMKYRYAGKEKLLSIGVYPDVTLAEARDKRTQAKRILAAGDD
PSEVKQAEREAKNLAVNNSFELLALEWHEHKKPNWSSGYADDILEYLRKDIFPYIGKKAITDIKPMTMLSVLKKMEDRGV
LDKLKKTRQACRQIFTYAIITGRAEFNPVTDLAGALKTPKQQHFPHLMPTQIGPFIHAVNTYSGSKVTRIATLLLMYTSV
RTIELRASEWTEFDLDNDLWQIPKERMKMRRPHLVPLSRQVKSHLLELKKITGWGKYVFPGRNDAHKPMSEASINQVIKR
IGFAGKVTGHGFRHTMSTILHEKGFNSAWIEAQLAHADRNTIRGTYNHAQYLDGRREMLQWYADYLDELVGKVVG

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=389, Percent_Identity=47.8149100257069, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI145693166, Length=392, Percent_Identity=40.5612244897959, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788974, Length=386, Percent_Identity=36.5284974093264, Blast_Score=268, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45243; Mature: 45243

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTARQISTAKPTEKPYKLSDGGGLYLLVNPNGSRYWRMKYRYAGKEKLLSIGVYPDVT
CCCCHHHCCCCCCCCCCEEECCCCCEEEEECCCCCEEEEEEEEECCCHHEEEECCCCCCH
LAEARDKRTQAKRILAAGDDPSEVKQAEREAKNLAVNNSFELLALEWHEHKKPNWSSGYA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHCCCCCCCCCCH
DDILEYLRKDIFPYIGKKAITDIKPMTMLSVLKKMEDRGVLDKLKKTRQACRQIFTYAII
HHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TGRAEFNPVTDLAGALKTPKQQHFPHLMPTQIGPFIHAVNTYSGSKVTRIATLLLMYTSV
CCCCCCCCHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RTIELRASEWTEFDLDNDLWQIPKERMKMRRPHLVPLSRQVKSHLLELKKITGWGKYVFP
HEEEEECCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECC
GRNDAHKPMSEASINQVIKRIGFAGKVTGHGFRHTMSTILHEKGFNSAWIEAQLAHADRN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
TIRGTYNHAQYLDGRREMLQWYADYLDELVGKVVG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLTARQISTAKPTEKPYKLSDGGGLYLLVNPNGSRYWRMKYRYAGKEKLLSIGVYPDVT
CCCCHHHCCCCCCCCCCEEECCCCCEEEEECCCCCEEEEEEEEECCCHHEEEECCCCCCH
LAEARDKRTQAKRILAAGDDPSEVKQAEREAKNLAVNNSFELLALEWHEHKKPNWSSGYA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHCCCCCCCCCCH
DDILEYLRKDIFPYIGKKAITDIKPMTMLSVLKKMEDRGVLDKLKKTRQACRQIFTYAII
HHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TGRAEFNPVTDLAGALKTPKQQHFPHLMPTQIGPFIHAVNTYSGSKVTRIATLLLMYTSV
CCCCCCCCHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RTIELRASEWTEFDLDNDLWQIPKERMKMRRPHLVPLSRQVKSHLLELKKITGWGKYVFP
HEEEEECCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECC
GRNDAHKPMSEASINQVIKRIGFAGKVTGHGFRHTMSTILHEKGFNSAWIEAQLAHADRN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
TIRGTYNHAQYLDGRREMLQWYADYLDELVGKVVG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]