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The map label for this gene is fadA [H]

Identifier: 194735609

GI number: 194735609

Start: 4066519

End: 4067682

Strand: Reverse

Name: fadA [H]

Synonym: SeSA_A4189

Alternate gene names: 194735609

Gene position: 4067682-4066519 (Counterclockwise)

Preceding gene: 194734920

Following gene: 194736748

Centisome position: 86.38

GC content: 58.68

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCTATTCGCACCCCGATGGGCCGTTCGAAGGGCGGCGCGTTTCGCAACGTGCGGGC
AGAAGATCTCTCCGCCCACTTAATGCGTAGCCTGCTGGCGCGTAATCCGTCGCTTACAGCGGCGACCCTCGATGATATTT
ACTGGGGCTGCGTACAACAAACGCTGGAGCAAGGCTTCAACATTGCCCGTAACGCCGCGCTGCTGGCAGAAATTCCCCAT
TCGGTACCGGCGGTCACCGTCAACCGTCTGTGTGGTTCCTCGATGCAGGCGTTACACGATGCAGCGCGAATGATCATGAC
CGGCGATGCGCAGGTTTGTCTGGTTGGCGGCGTGGAGCATATGGGGCACGTGCCGATGAGTCACGGCGTGGATTTTCACC
CGGGTCTGAGTCGCAACGTCGCCAAAGCGGCAGGGATGATGGGGCTAACGGCGGAAATGCTCTCCCGCCTGCACGGCATT
AGCCGGGAAATGCAGGACCAGTTCGCCGCGCGTTCTCACGCTCGCGCCTGGGCCGCCACCCAGTCTGGTGCATTCAAAAC
GGAGATTATCCCAACTGGCGGTCATGATGCAGACGGCGTGTTGAAGCAGTTTAGCTACGATGAAGTGATCCGCCCGGAAA
CCACGGTCGAAGCGCTATCAACGCTGCGTCCGGCATTTGATCCGGTTAGTGGCACGGTCACGGCGGGCACCTCATCCGCG
CTTTCCGATGGCGCAGCCGCCATGCTGGTAATGAGCGAAAGTCGTGCCCGTGAGCTGGGCCTGAAACCTCGCGCCCGTAT
TCGTTCAATGGCAGTGGTTGGTTGTGATCCGTCAATTATGGGTTACGGGCCGGTTCCGGCGTCAAAACTGGCGTTGAAAA
AAGCGGGACTGTCGGCCAGCGATATCGATGTGTTTGAGATGAACGAAGCGTTTGCCGCACAGATCCTGCCATGCATTAAG
GATCTGGGATTGATGGAGCAGATAGACGAGAAGATCAACCTCAACGGCGGCGCGATCGCGCTTGGTCATCCGCTCGGCTG
CTCCGGAGCACGTATCAGCACCACGCTTATCAACCTGATGGAGCGCAAAGACGCGCAGTTTGGTCTGGCGACGATGTGTA
TTGGTCTGGGTCAGGGCATCGCCACGGTGTTTGAGCGGGTTTAA

Upstream 100 bases:

>100_bases
GGAGGGGCTGCGTAATAAAGCGCGCCATAACGAACCGTATTATCCCCCGGTTGAACCAGCCCGTCCGGTTGGTTCTCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
TCAAAGATAATATCCCCTAAATAACTCGCATTGCAGCGCAGCGGCAACTGAGCGAATCCCCGGGAACATAGATAACTATG
TGCCCGGGGTGAGCAAAGGC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPSLTAATLDDIYWGCVQQTLEQGFNIARNAALLAEIPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLSRLHGI
SREMQDQFAARSHARAWAATQSGAFKTEIIPTGGHDADGVLKQFSYDEVIRPETTVEALSTLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIDVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLMERKDAQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPSLTAATLDDIYWGCVQQTLEQGFNIARNAALLAEIPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLSRLHGI
SREMQDQFAARSHARAWAATQSGAFKTEIIPTGGHDADGVLKQFSYDEVIRPETTVEALSTLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIDVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLMERKDAQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPSLTAATLDDIYWGCVQQTLEQGFNIARNAALLAEIPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLSRLHGI
SREMQDQFAARSHARAWAATQSGAFKTEIIPTGGHDADGVLKQFSYDEVIRPETTVEALSTLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIDVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLMERKDAQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=42.9292929292929, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=39.746835443038, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=39.4472361809045, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=34.2657342657343, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.3283582089552, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.8066157760814, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=94.8320413436692, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.9259259259259, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=43.7037037037037, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.1471321695761, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=34.7517730496454, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=40.3990024937656, Blast_Score=267, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.953488372093, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=31.5789473684211, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=387, Percent_Identity=27.906976744186, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=40.4092071611253, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=35.2941176470588, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.2985074626866, Blast_Score=260, Evalue=9e-70,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.3034825870647, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.4988344988345, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24640423, Length=402, Percent_Identity=32.3383084577114, Blast_Score=165, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40978; Mature: 40978

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPSLTAATLDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEIPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEECCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLSRLHGISREMQDQFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSGAFKTEIIPTGGHDADGVLKQFSYDEVIRPETTVEALSTLRPAFDPVSGTVTAGTSSA
CCCCEEEEEEECCCCCHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIDVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLM
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDAQFGLATMCIGLGQGIATVFERV
HHHCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPSLTAATLDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEIPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEECCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLSRLHGISREMQDQFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSGAFKTEIIPTGGHDADGVLKQFSYDEVIRPETTVEALSTLRPAFDPVSGTVTAGTSSA
CCCCEEEEEEECCCCCHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIDVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLM
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDAQFGLATMCIGLGQGIATVFERV
HHHCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA