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The map label for this gene is kgtP [H]

Identifier: 194735580

GI number: 194735580

Start: 2746989

End: 2748290

Strand: Reverse

Name: kgtP [H]

Synonym: SeSA_A2848

Alternate gene names: 194735580

Gene position: 2748290-2746989 (Counterclockwise)

Preceding gene: 194734377

Following gene: 194735064

Centisome position: 58.36

GC content: 51.84

Gene sequence:

>1302_bases
ATGGCAGAAATTATTCAGCGTATAGATAAAAACAACGCTGAGGCTCCGGAGATGCGGCGACGCATATGGGCTATCGTGGG
GGCATCTTCCGGAAACTTAGTGGAATGGTTTGATTTCTACGTCTACTCTTTCTGCTCGCTCTATTTTGCACATATCTTCT
TTCCTTCTGGTAATACCACCACTCAGCTTTTGCAAACGGCAGGCGTCTTTGCCGCCGGTTTTTTAATGCGACCTATCGGT
GGTTGGCTGTTTGGACGCATTGCCGATCGACGCGGGCGCAAAGCCTCAATGTTGATTTCGGTGTGCATGATGTGCATGGG
GTCGCTGGTGATTGCCTGCTTACCTGGTTACGATACCATCGGCACCTGGGCACCGGCGCTGCTACTTGTTGCAAGGTTAT
TTCAGGGGCTCTCGGTAGGGGGAGAGTATGGGACCAGCGCGACATATATGAGTGAAGTTGCGCTTGAAGGTCGTAAGGGA
TTTTTCGCGTCGTTTCAGTACGTTACGCTTATCGGTGGCCAACTACTGGCCTTGCTGGTCGTTGTGATTTTGCAGCAGGT
TCTGGAAGATGCTGAACTTCGTGCCTGGGGATGGCGTATTCCCTTTGCGTTGGGCGCCGTGCTGGCAGTTGTGGCGTTAT
GGCTACGTCGCCAGCTGGATGAAACCTCGAAGCATGAGACTCGCGCGTTAAAAGAAGCCGGTTCATTGAAAGGTTTGTGG
CGTAATCGCAAAGCCTTTTTGATGGTGCTTGGTTTCACCGCGGCAGGCTCGCTGTGCTTTTATACCTTCACCACCTATAT
GCAGAAATATCTGGTAAATACAGCAGGTATGCATGCCAATGTCGCCAGCGGCATTATGACGGTCGCGCTGTGTGTTTTCA
TGTTGGTGCAGCCGTTATTCGGCGCGTTATCCGATAAGATTGGCAGGCGCACTTCTATGCTATGTTTTGGCGCGCTCGCG
ACGCTTTTCACCGTTCCAATTCTCTCCGCGTTGCAGAATGTGACGTCGCCTTATGCCGCATTTGCTTTAGTGATGTGCGC
GCTATTGATTGTGAGTTTCTATACCTCGATTAGCGGGATCCTGAAAGCGGAGATGTTTCCGGCGCAGGTTCGCGCCCTGG
GCGTCGGTTTATCCTATGCGGTAGCAAACGCGTTATTTGGCGGCTCTGCGGAATATGTCGCGCTGTCGCTAAAATCTGTC
GGTATGGAGAGTTCATTCTTCTGGTATGTTACCGCGATGGCCGTTCTGGCTTTCCTGGTTTCGCTGATGTTGCACCGTAA
AGGAAAGGGCTTGCGCCTTTAG

Upstream 100 bases:

>100_bases
TTAGCAAACAAATAATTAACACTTTATTTACTTTTTGGTCTCGCGTAATCTGAATCTCCAAAATAAAAGGCCGATTAAGC
CTGGCAACAAGGAGAACAGT

Downstream 100 bases:

>100_bases
TATCGCGCCATTTGCCAGATAGCGTAACCGGTTGTCGCGCCAGCGACATCCCAGACAAAATCTTTCCAGCTCCAGCCGCT
GCCTTCCGGGCGGCTGTCCC

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAEIIQRIDKNNAEAPEMRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIG
GWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLVARLFQGLSVGGEYGTSATYMSEVALEGRKG
FFASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTVALCVFMLVQPLFGALSDKIGRRTSMLCFGALA
TLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSV
GMESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL

Sequences:

>Translated_433_residues
MAEIIQRIDKNNAEAPEMRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIG
GWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLVARLFQGLSVGGEYGTSATYMSEVALEGRKG
FFASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTVALCVFMLVQPLFGALSDKIGRRTSMLCFGALA
TLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSV
GMESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL
>Mature_432_residues
AEIIQRIDKNNAEAPEMRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGG
WLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLVARLFQGLSVGGEYGTSATYMSEVALEGRKGF
FASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLWR
NRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTVALCVFMLVQPLFGALSDKIGRRTSMLCFGALAT
LFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSVG
MESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=433, Percent_Identity=88.6836027713626, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=363, Percent_Identity=33.0578512396694, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788292, Length=401, Percent_Identity=31.4214463840399, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=32.5526932084309, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87082231, Length=310, Percent_Identity=26.7741935483871, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=24.5333333333333, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47281; Mature: 47149

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIIQRIDKNNAEAPEMRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTT
CHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TQLLQTAGVFAAGFLMRPIGGWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GTWAPALLLVARLFQGLSVGGEYGTSATYMSEVALEGRKGFFASFQYVTLIGGQLLALLV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTVALCVFMLVQPLF
HCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GALSDKIGRRTSMLCFGALATLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSVGMESSFFWYVTAMAVLAFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SLMLHRKGKGLRL
HHHHHCCCCCCCC
>Mature Secondary Structure 
AEIIQRIDKNNAEAPEMRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTT
HHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH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HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]