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The map label for this gene is cueO [H]

Identifier: 194735559

GI number: 194735559

Start: 195428

End: 197038

Strand: Direct

Name: cueO [H]

Synonym: SeSA_A0187

Alternate gene names: 194735559

Gene position: 195428-197038 (Clockwise)

Preceding gene: 194738300

Following gene: 194736226

Centisome position: 4.15

GC content: 55.8

Gene sequence:

>1611_bases
ATGTTACGCCGTGATTTCTTAAAATATTCAGTGGCGCTGGGGGTTGCATCAGCGCTGCCGCTGTGGAGCCGCGCCGCTTT
TGCCGCCGAACGTCCCGCGTTGCCTATTCCTGACCTGTTAACGGCAGATGCGAGCAACCGTATGCAGTTAATTGTTAAAG
CCGGACAGTCGACATTCGCCGGTAAGAACGCGACAACCTGGGGCTACAACGGTAATTTGCTGGGGCCAGCGGTACAGCTT
CACAAAGGAAAAAGTGTGACCGTTGATATCCATAACCAACTGGCCGAAGACACGACGCTTCACTGGCATGGTCTGGAGAT
TCCGGGCATCGTCGACGGCGGCCCGCAGGGGATTATTCCCGCAGGCGGAACCCGCACGGTGACGTTTACGCCGGAGCAAC
GCGCCGCGACCTGCTGGATTCATCCACATAAACACGGCAAAACCGGGCGCCAGGTGGCGATGGGCCTTGCCGGACTGGTG
CTGATTGAAGATGACGAGATTCGCAAATTGCGCCTGCCGAAACAGTGGGGCATCGACGATGTGCCGGTGATCATTCAGGA
CAAACGCTTCTCCGCCGATGGTCAGATTGATTATCAACTGGATATTATGACCGCCGCCGTCGGCTGGTTTGGCGATACGC
TGCTGACCAATGGCGCTATCTATCCGCAGCATTCCGCGCCGAAAGGCTGGCTACGTCTGCGCTTGCTAAATGGCTGTAAT
GCGCGTTCGCTGAATATCGCCGCCAGCGATAATCGCCCGCTTTATGTGATCGCCAGCGACGGCGGCCTGCTGGCGGAGCC
GGTGAAAGTCACCGAACTGCCGTTATTAATGGGCGAGCGTTTTGAAGTGCTGGTGGATATCAGCGACGGGAAAGCCTTTG
ATCTGGTGACCCTGCCGGTCAGCCAGATGGGAATGGCGATCGCTCCGTTTGATAAACCGCATCCGGTGATGCGTATCCAG
CCGCTGCGGATTACCGCCTCCGGTACGCTGCCGGATACGTTGACGACGATGCCGGCGTTGCCGTTGCTGGAAGGGCTGAC
GGTGCGCAACCTGAAACTGTCGATGGACCCGCGTCTTGATATGATGGGGATGCAAATGCTGATGAAGAAATATGGCGCTC
AGGCGATGAGCGGTATGGATCATGACAGCATGAACGCGCATATGCAGGGCGGCAATATGGGGCATGGCGAGATGGATCAT
GGCAACATGGATCACAGCGGGATGAATCATGGCGCGATGGGCAATATGAATCACGGCGGAAAATTCGACTTCCATAACGC
TAACTTTATCAACGGCCAGGTCTTCGATATGAACAAACCGATGTTCGCGGCGCAAAAAGGCCGACATGAACGTTGGGTGA
TTTCCGGCGTGGGCGACATGATGCTGCATCCTTTCCATATTCACGGCACGCAGTTCCGTATTCTGTCAGAGAACGGCAAA
GCGCCAGCAGCGCACAGAACGGGCTGGAAGGATACGGTACGCGTTGAGGGCGGTATCAGCGAAGTGTTGGTCAAGTTCGA
TCACGACGCGCCGAAGGAACATGCCTATATGGCGCACTGTCATCTGTTAGAACATGAAGATACGGGAATGATGTTAGGAT
TTACGGTCTAA

Upstream 100 bases:

>100_bases
ATTGCTGTCAGGAATGGTAAAGACGTGTTGCTGGCTTGACCTTCCCGTTAGGGCAGGGTCTAAGCTTAGACACCCGCCTG
TTCATTATAAGGAAATGATT

Downstream 100 bases:

>100_bases
TCGCTGTCTCCGACGCAGGCCGGGTAAGGAGAAGCCGCCACCCGGCACAATGCCCGGCATACGCCGGGCATTAATGGTTA
TTTCGCGTCGTCAGGCAAAG

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPEQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPLLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV

Sequences:

>Translated_536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPEQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPLLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
>Mature_536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPEQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPLLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=536, Percent_Identity=81.7164179104478, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=391, Percent_Identity=33.5038363171356, Blast_Score=205, Evalue=6e-54,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 58653; Mature: 58653

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEECCCCCCC
GKNATTWGYNGNLLGPAVQLHKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIP
CCCCEECCCCCCCCCCEEEEECCCEEEEEEHHHCCCCCEEEEECCCCCCEECCCCCCEEC
AGGTRTVTFTPEQRAATCWIHPHKHGKTGRQVAMGLAGLVLIEDDEIRKLRLPKQWGIDD
CCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCEEEECCHHCCCCC
VPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
CCEEEECCCCCCCCEEEEEEEEEEHHHHHHCHHHHCCCEECCCCCCCCCEEEEEEECCCC
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPV
CCEEEEEECCCCCEEEEECCCCEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEEEECH
SQMGMAIAPFDKPHPVMRIQPLRITASGTLPDTLTTMPALPLLEGLTVRNLKLSMDPRLD
HHCCEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHHCCCEEEEEEEECCCCHH
MMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDHGNMDHSGMNHGAMGNMNHGG
HHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
EEECCCCCEECCEEEECCCCCHHHHCCCCCEEEEECCCCHHCCEEEECCEEEEEEECCCC
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
CCCCCCCCCCCEEEECCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEECCCCCCC
GKNATTWGYNGNLLGPAVQLHKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIP
CCCCEECCCCCCCCCCEEEEECCCEEEEEEHHHCCCCCEEEEECCCCCCEECCCCCCEEC
AGGTRTVTFTPEQRAATCWIHPHKHGKTGRQVAMGLAGLVLIEDDEIRKLRLPKQWGIDD
CCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCEEEECCHHCCCCC
VPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
CCEEEECCCCCCCCEEEEEEEEEEHHHHHHCHHHHCCCEECCCCCCCCCEEEEEEECCCC
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPV
CCEEEEEECCCCCEEEEECCCCEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEEEECH
SQMGMAIAPFDKPHPVMRIQPLRITASGTLPDTLTTMPALPLLEGLTVRNLKLSMDPRLD
HHCCEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHHCCCEEEEEEEECCCCHH
MMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDHGNMDHSGMNHGAMGNMNHGG
HHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
EEECCCCCEECCEEEECCCCCHHHHCCCCCEEEEECCCCHHCCEEEECCEEEEEEECCCC
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
CCCCCCCCCCCEEEECCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]